Construction of a Recombinant Plasmid for Petal-Specific Expression of HQT, a Key Enzyme in Chlorogenic Acid Biosynthesis

  • Yuting Bi
  • Wei Tian
  • Wen Zeng
  • Yushan Kong
  • Yanhong Xue
  • Shiping Liu
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 269)

Abstract

The flower buds of Lonicera japonica are widely used in Chinese medicine due to the anti-inflammatory properties. Chlorogenic acid (CGA) may be one of the most critical components in L. japonica. Previous studies suggest that HQT gene play a very important role in the synthesis of CGA. In order to overexpress HQT gene specifically in the petals, we successfully isolated the full-length cDNA of HQT gene from L. japonica. Then from Lilium orential ‘Sorbonne’, we cloned an 896-bp promoter (PLoCHS) which can drive chalcone synthase gene (CHS) specifically expression in flowers. After sequencing and bioinformatical analysis, two constructs based on pCAMBIA2300 were obtained, one was a constituently expression vector driving HQT by 35S promoter, and the other fused HQT and PLoCHS promoter. In the further research, we will transform the constructs into L. japonica callus. This study provides a means of improving the CGA content in L. japonica flowers, and will be helpful in understanding the expression pattern of HQT related with CGA and development of the plants.

Keywords

HQT PLoCHS CGA Construction Petal 

Notes

Acknowledgments

Thanks to the support of China Three Gorges University for the preliminary study. This project was supported by the National Natural Science Foundation of China (31270389) and Natural Science Research of Hubei Education Department (D20121301).

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Yuting Bi
    • 1
  • Wei Tian
    • 1
  • Wen Zeng
    • 1
  • Yushan Kong
    • 1
  • Yanhong Xue
    • 1
  • Shiping Liu
    • 1
  1. 1.College of Chemistry and Life ScienceChina Three Gorges UniversityYichangPeople’s Republic of China

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