Molecular Cloning, Sequence Analysis of Thioesterases from Wintersweet (Chimonanthus Praecox)
A coding region of wintersweet cDNA was cloned via screening a wintersweet cDNA library. The CpFATB cDNA is 1110 bp in length with an open reading frame (ORF) of 1107 bp encoding a protein of 369 amino acids. Molecular weight and isoelectric point of the protein is 41.72 kD and 7.72, respectively. Bioinformatics analysis shows that a signal peptide with 49 amino acid residues and Protein Localization Sites exists in the chloroplast stroma. This classifies the protein as stable.
KeywordsThioesterases Bioinformatics Wintersweet
- 1.Ohlrogge JB, Kuhn DN (1979) Subcellular localization of acyl carrier protein in leaf protoplasts of Spinacia oleracea. Proc Natl Acad Sci USA 76:1194–1198Google Scholar
- 2.Voelker TA, Jones A, Cranmer AM, et al (1997) Broad-range and binary-range acyl–acyl–carrier protein thioestera sessuggest an alternative mechanism for medium-chain production in seeds. Plant Physiol 114:669–677Google Scholar
- 5.Zhang SH, Wei Y, Liu JL, et al (2011) CpCHT1 an apoplastic chitinase isolated from the corolla of wintersweet (Chimonanthuspraecoxcommunis L.) exhibits both antifungal and antifreeze activities. Biol Plantarum 55:141–148Google Scholar