Molecular Cloning, Sequence Analysis of Thioesterases from Wintersweet (Chimonanthus Praecox)

  • Li-Hong Zhang
  • Qiong Wu
  • Xian-Feng Zou
  • Li-Na Chen
  • Shu-Yan Yu
  • Chang-Cheng Gao
  • Xing Chen
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 269)

Abstract

A coding region of wintersweet cDNA was cloned via screening a wintersweet cDNA library. The CpFATB cDNA is 1110 bp in length with an open reading frame (ORF) of 1107 bp encoding a protein of 369 amino acids. Molecular weight and isoelectric point of the protein is 41.72 kD and 7.72, respectively. Bioinformatics analysis shows that a signal peptide with 49 amino acid residues and Protein Localization Sites exists in the chloroplast stroma. This classifies the protein as stable.

Keywords

Thioesterases Bioinformatics Wintersweet 

References

  1. 1.
    Ohlrogge JB, Kuhn DN (1979) Subcellular localization of acyl carrier protein in leaf protoplasts of Spinacia oleracea. Proc Natl Acad Sci USA 76:1194–1198Google Scholar
  2. 2.
    Voelker TA, Jones A, Cranmer AM, et al (1997) Broad-range and binary-range acyl–acyl–carrier protein thioestera sessuggest an alternative mechanism for medium-chain production in seeds. Plant Physiol 114:669–677Google Scholar
  3. 3.
    Dörmann P, Voelker TA, Ohlrogge JB (2000) Accumulation of palmitate in Arabidopsis mediated by the acyl–acyl carrier protein thioesterase FATB1. Plant Physiol 123:637–643CrossRefGoogle Scholar
  4. 4.
    Salas JJ, Ohlrogge JB (2002) Characterisation of substrate specificity of plant FATA and FATB acyl-ACP thioesterases. Arch Biochem Biophys 403:25–34CrossRefGoogle Scholar
  5. 5.
    Zhang SH, Wei Y, Liu JL, et al (2011) CpCHT1 an apoplastic chitinase isolated from the corolla of wintersweet (Chimonanthuspraecoxcommunis L.) exhibits both antifungal and antifreeze activities. Biol Plantarum 55:141–148Google Scholar
  6. 6.
    Dillon SC, Bateman A (2004) The Hotdog fold: wrapping up a superfamily of thioesterases and dehydratases. BMC Bioinforma 5:109CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Li-Hong Zhang
    • 1
  • Qiong Wu
    • 1
  • Xian-Feng Zou
    • 1
  • Li-Na Chen
    • 1
  • Shu-Yan Yu
    • 1
  • Chang-Cheng Gao
    • 1
  • Xing Chen
    • 1
  1. 1.Key Laboratory of Agricultural Products ProcessingChangchun UniversityChangchunPeople’s Republic of China

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