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Molecular Cloning, Sequence Analysis of Thioesterases from Wintersweet (Chimonanthus Praecox)

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Frontier and Future Development of Information Technology in Medicine and Education

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 269))

Abstract

A coding region of wintersweet cDNA was cloned via screening a wintersweet cDNA library. The CpFATB cDNA is 1110 bp in length with an open reading frame (ORF) of 1107 bp encoding a protein of 369 amino acids. Molecular weight and isoelectric point of the protein is 41.72 kD and 7.72, respectively. Bioinformatics analysis shows that a signal peptide with 49 amino acid residues and Protein Localization Sites exists in the chloroplast stroma. This classifies the protein as stable.

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Correspondence to Li-Hong Zhang .

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© 2014 Springer Science+Business Media Dordrecht

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Zhang, LH. et al. (2014). Molecular Cloning, Sequence Analysis of Thioesterases from Wintersweet (Chimonanthus Praecox). In: Li, S., Jin, Q., Jiang, X., Park, J. (eds) Frontier and Future Development of Information Technology in Medicine and Education. Lecture Notes in Electrical Engineering, vol 269. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7618-0_327

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  • DOI: https://doi.org/10.1007/978-94-007-7618-0_327

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  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-007-7617-3

  • Online ISBN: 978-94-007-7618-0

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