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Construction of Expression Vector of miRNA Specific for FUT3 and Identification of Its Efficiency in KATO-III Gastric Cancer Cell Line

  • Yong-Hong Xin
  • Yan-Fei Jia
  • Qiang Liu
  • Hong Zhang
  • Hai-ning Zhu
  • Xiao-li Ma
  • Yong-Jun Cai
  • Yun-Shan Wang
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 269)

Abstract

Human blood group Lewis antigen, which is fucosylated glycoconjugates involved in the development of several pathologies. Fucosyltransferases are enzymes that add fucose to precursor glycan structures: FUT3 catalyze the addition of fucose to the α 1-3, 4 position and are detected in epithelial cells. As the expression of Lewis a is mainly controlled by FUT3, which can influence Lewis a’S synthesis, we choose FUT3 as our target gene to silence. In our study, we constructed FUT3-specific miRNA expression vector successfully. It showed in the experiment that FUT3—specific miRNA expression vector could actively inhibit FUT3’S expression in mRNA level in KATO—III gastric cancer cel1 line, and decrease Lewis antigen’S synthesis as welI growth. From this experiment, we obtain the best mi RNA sequence of FUT3, which may serve as a new strategy for investigating the mechanism of molecular glycol—pathology of gastric cancer targeted tumor gene therapy.

Keywords

FUT3 Lewis antigen Gastric cancer miRNA KATO-III RNAi 

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Yong-Hong Xin
    • 1
  • Yan-Fei Jia
    • 2
  • Qiang Liu
    • 3
  • Hong Zhang
    • 4
  • Hai-ning Zhu
    • 5
  • Xiao-li Ma
    • 2
  • Yong-Jun Cai
    • 1
  • Yun-Shan Wang
    • 2
  1. 1.Department of Blood Transfusion, Fourth People’s Hospital of JinanTaishan Medical CollegeJinanChina
  2. 2.Shandong UniversityCentral Laboratory, Jinan Central HospitalJinanChina
  3. 3.China National Heavy Duty Truck Group Corporation HospitalJinanChina
  4. 4.Clinical LaboratoryJinanChina
  5. 5.Zi Bo Municipal Center For Disease Control and PreventionZiboChina

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