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Cloning and Characterization of Two cDNA Sequences Coding Squalene Synthase Involved in Glycyrrhizic Acid Biosynthesis in Glycyrrhiza uralensis

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Frontier and Future Development of Information Technology in Medicine and Education

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 269))

Abstract

Glycyrrhiza uralensis are widely used in Chinese medicine for the functions of relieving cough, clearing heat and detoxicating. They are also used as food additives and industry materials. There are various components with different pharmacological activities in G. uralensis, among them glycyrrhizic acid is believed as an indicator component to characterize the quality of this herb. In the present study, two cDNA sequences coding squalene synthase (SQS1 and SQS2) involved in glycyrrhizic acid biosynthesis in G. uralensis were cloned and expressed in Escherichia coli as fusion proteins, which showed the similar activity as SQS isolated from other species to catalyze farnesyl diphosphate (FPP) into squalene (SQ). Because SQS is a key enzyme in glycyrrhizic acid metabolic pathway in G. uralensis, this work is significant for further studies concerned with exploring the molecular mechanism of biosynthesis of glycyrrhizic acid and improving the quality of G. uralensis.

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Abbreviations

SQS:

Squalene synthase

SQ:

Squalene

FPP:

Farnesyl diphosphate

ORF:

Open reading frame

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Authors and Affiliations

  1. School of Chinese Pharmacy, Beijing University of Chinese Medicine, No. 6 South Wangjing Zhonghuan Road, Beijing, China

    Ying Liu, Ning Zhang, Honghao Chen, Ya Gao, Hao Wen, Yong Liu & Chunsheng Liu

Authors
  1. Ying Liu
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  2. Ning Zhang
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  3. Honghao Chen
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  4. Ya Gao
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  5. Hao Wen
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  6. Yong Liu
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  7. Chunsheng Liu
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Editor information

Editors and Affiliations

  1. Cognitive Science, Xiamen University, Xiamen, People's Republic of China

    Shaozi Li

  2. Human Informatics and Cognitive Sciences, Waseda University Networked Information Systems Lab, Waseda, Japan

    Qun Jin

  3. School of Systems Information Science, Future University Hakodate, Hakodate, Hokkaido, Japan

    Xiaohong Jiang

  4. Department of Computer Science and Engineering, Seoul University of Science & and Technology (SeoulTech), Seoul, Korea, Republic of (South Korea)

    James J. (Jong Hyuk) Park

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Liu, Y. et al. (2014). Cloning and Characterization of Two cDNA Sequences Coding Squalene Synthase Involved in Glycyrrhizic Acid Biosynthesis in Glycyrrhiza uralensis . In: Li, S., Jin, Q., Jiang, X., Park, J. (eds) Frontier and Future Development of Information Technology in Medicine and Education. Lecture Notes in Electrical Engineering, vol 269. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7618-0_32

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  • DOI: https://doi.org/10.1007/978-94-007-7618-0_32

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  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-007-7617-3

  • Online ISBN: 978-94-007-7618-0

  • eBook Packages: EngineeringEngineering (R0)

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