Abstract
Objective Analyse the biological performance of the PRP/nHA/CoI composite biofilm. Methods Prepared nHA(nHA, Nanosized hydroxyapatit) by Chemical precipitation; Co- I (Co- I, Collagen I) was extracted by acid dissolution and protease digestion; Got platelet rich plasma (PRP, Platelet-Rich Plasma) by centrifugation. And crosslinked the composite material. Use the scanning electron microscopy, ELISA immunohistochemistry, 16-slice msct (msct, Multislice Spiral Computed Tomography) to observe composite material and analysis by computer software. Results PRP/nHA/CoI has a higher porosity in line with the tissue engineering material.
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References
Liu K, Meng H, Hou J (2012) Activity of 25-hydroxylase in human gingival fibroblasts and periodontal ligament cells. PLoS ONE 7(12):e52053
Woo KM, Chen VJ, Jung HM, Kim TI, Shin HI, Baek JH et al (2009) Tissue Eng Part A 15(8):2155e62
Seyedjafari E, Soleimani M, Ghaemi N, Shabani I (2010) Biomacromolecules 11(11):3118e25
Bohner M (2010) Design of ceramic-based cements and putties for bone graft substitution. Eur Cells Mater 20:1–12
Johnson AJW, Herschler BA (2011) Acta Biomater 7:16–30
Gałkowska E, Kiernicka M, Owczarek B (2003) The use of HA-Biocer in the complex treatment of aggressive periodontal diseases. Ann Univ Mariae Curie Sklodowska Med. 58(1):231–235
Zietek M, Gedrange T, Mikulewicz M (2008) Long term evaluation of biomaterial application in surgical treatment of periodontosis. J Physiol Pharmacol 59(5):81–86
Koutouzis T, Haber D, Shaddox L, Aukhil I, Wallet SM (2009) Autoreactivity of serum immunoglobulin to periodontal tissue components: a pilot study. J Periodontol 80(4):625–633
Hendler A, Mulli TK, Hughes FJ (2010) Involvement of autoimmunity in the pathogenesis of aggressive periodontitis. J Dent Res 89(12):1389–1394
Marelli B, Ghezzi CE, Barralet JE, Boccaccini AR, Nazhat SN (2010) Biomacromolecules 11:1470e9
Chen R, Hunt JA (2007) Biomimetic materials processing for tissue-engineering processes. J Mater Chem 17:3974e9
Kang J, Sha YQ, Ou-yang XY (2010) Combination therapy of periodontal intrabony defects with demineralized freeze-dried bone powder and platelet-rich plasma. Beijing Da Xue Xue Bao (Chinese) 42(1):24–27
Pepelassi EA, Markopoulou CE, Dereka XE (2009) Platelet-rich plasma effect on periodontally affected human gingival fibroblasts: an in vitro study. J Int Acad Periodontol 11(1):160–8
Chen K, Ng KS, Ravi S, Goh JC, Toh SL (2013) In vitro generation of whole osteochondral constructs using rabbit bone marrow stromal cells, employing a two-chambered co-culture well design. J Tissue Eng Regen Med
Roostaeian J, Carlsen B, Simhaee D (2006) Characterization of growth and osteogenic differentiation of rabbit bone marrow stromal cells. J Surg Res 133(2):76–83
Jiang XQ, Chen JG, Gittens S (2005) The ectopic study of tissue-engineered bone with hBMP-4 gene modified bone marrow stromal cells in rabbits. Chin Med J (Engl) 118(4):281–288
Zhang C, Ma Q, Qiang H (2010) Study on effect of recombinant adeno-associated virus vector co-expressing human vascular endothelial growth factor 165 and human bone morphogenetic protein 7 genes on bone regeneration and angiopoiesis in vivo. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (Chinese) 24(12):1449–1454
Tamaki Y, Nakahara T, Ishikawa H, Sato S (2012) In vitro analysis of mesenchymal stem cells derived from human teeth and bone marrow. Odontology
JulKim SH, Kim YS, Lee SY (2011) Gene expression profile in mesenchymal stem cells derived from dental tissues and bone marrow. J Periodontal Implant Sci 41(4):192–200
Kim SH, Kim KH, Seo BM (2009) Alveolar bone regeneration by transplantation of periodontal ligament stem cells and bone marrow stem cells in a canine peri-implant defect model: a pilot study. J Periodontol 80(11):1815–1823
Li H, Yan F, Lei L, Li Y, Xiao Y (2009) Application of autologous cryopreserved bone marrow mesenchymal stem cells for periodontal regeneration in dogs. Cells Tissues Organs 190(2):94–101
Ueno T, Kagawa T, Ishida N (2001) Prefabricated bone graft induced from grafted periosteum for the repair of jaw defects: an experimental study in rabbits. J Craniomaxillofac Surg 29(4):219–223
Matsumoto G, Hoshino J, Kinoshita Y (2012) Evaluation of guided bone regeneration with poly(lactic acid-co-glycolic acid-co-ε-caprolactone) porous membrane in lateral bone defects of the canine mandible. Int J Oral Maxillofac Implants 27(3):587–594
Martinez LA, Gioso MA, Lobos CM, Pinto AC.Localization of the mandibular canal in brachycephalic dogs using computed tomography.J Vet Dent. 2009 Fall;26(3):156-163
Soukup JW, Lawrence JA, Pinkerton ME, Schwarz T (2009) Computed tomography-assisted management of a mandibular dentigerous cyst in a dog with a nasal carcinoma. J Am Vet Med Assoc 235(6):710–714
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Ma, N. et al. (2014). Biological Performance Evaluation of the PRP/nHA/CoI Composite Material. In: Li, S., Jin, Q., Jiang, X., Park, J. (eds) Frontier and Future Development of Information Technology in Medicine and Education. Lecture Notes in Electrical Engineering, vol 269. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7618-0_303
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DOI: https://doi.org/10.1007/978-94-007-7618-0_303
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