The Reverse Effects of Saikoside on Multidrug Resistance
Aim To research the reverse effects of saikoside (SS) on the multidrug resistance (MDR) of human leukemic cell line K562/ADM and the related mechanism. METHODS K562 cells and K562/ADM cells in the culture were treated with SS at the concentrations of 1–100 mg/L. The inhibitory rate of the cell proliferation was measured by MTT assay. Non-cytotoxic dose of SS was determined. K562/ADM cells were treated with SS at non-cytotoxic doses of 1.25, 2.5 and 5.0 mg/L with different concentrations of adriamycin (ADM, 0.05–100 mg/L). The 50 % inhibitory concentration (IC50) and the reversal index in all groups were determined. The cell morphology was observed after treated with SS + ADM. The effects of SS on ADM accumulation in K562/ADM cells, the cell cycle profile was examined by flow cytometry. Results The inhibitory rates were significantly increased in a dose-dependent manner when the cells were treated with different doses of SS(1–100 mg/L). The available reversal concentration of SS was 5.0 mg/L and the reversal index was 21.5 folds for K562/ADM cells. After treated with SS + ADM, the number of tumor cells was decreased and aoptotic cells were increased in a dose–response relationship. ADM accumulation in K562/ADM cells treated with SS was significantly higher than that in control cells (P < 0.05). K562/ADM cells treated with SS were blocked in the stage of G0/G1. Conclusion SS has effect on proliferation inhibition and MDR reversal in K562/ADM cell line. The reversal mechanisms of SS may be due to increasing the accumulation of chemotherapeutics in the cell, and arresting the cells in G0/G1 phase.
KeywordsSaikoside Multidrug resistance Reversal of drug resistance K562/ADM cells
The work was funded by Science and Technology Department of Jilin Province (20110728), science and technology project of Jilin city (201262501) and department of education of Jilin Province (2013193).
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