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A Biomedical Microdevice for Quantal Exocytosis Measurement with Microelectrodes Arrays

  • Liguo Sun
  • Zhimeng Zou
  • Haifei Li
  • Peizheng Liu
  • Keping Tan
  • Jun Li
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 269)

Abstract

A microdevice was developed for cell trapping and exocytosis monitoring. The microdevice was assembled by two-layer poly(dimethyl siloxane) slab and microelectrodes integrated glass slide. The top layer with a depth of 20 μm was manufactured for cell flowing and the bottom layer with a depth of 2.5 μm was manufactured for cell trapping. Chromaffin cells can be trapped on top of indium tin oxide electrodes with this microdevice, and amperometric signals originated from catecholamine oxidation were obtained by flowing high K+ stimulating solution. The time course and areas of spikes recorded show that the manufactured device can provide sufficient sensitivity for quantal exocytosis measurement, and this biomedical device can be used for measuring cell exocytosis as traditional carbon fiber microelectrodes. By coupling with the multi-channel amplifier, high throughput analysis of cell exocytosis can be achieved with this microdevice, which is obviously superior to the carbon fiber microelectrodes based method.

Keywords

Microdevice Exocytosis Poly(dimethyl siloxane) Chromaffin cells 

References

  1. 1.
    Kennedy RT, Huang L, Atkinson MA, Dush P (1993) Detection of exocytosis at individual pancreatic beta cells by amperometry at a chemically modified microelectrode. Anal Chem 65:1882–1886CrossRefGoogle Scholar
  2. 2.
    Dod JT, Schroeder J, Borges R, Finnegan JM, Pihel K, Amatore C, Wightman RM (1996) Biophys J 70:1061–1068CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Liguo Sun
    • 1
  • Zhimeng Zou
    • 1
  • Haifei Li
    • 1
  • Peizheng Liu
    • 1
  • Keping Tan
    • 1
  • Jun Li
    • 1
  1. 1.Department of RadiologyBinzhou Medical College Affiliated Hospital at YantaiYantaiChina

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