Research on the Pharmacokinetics and Elimination of Epigallocatechin Gallate (EGCG) in Mice

  • Yang Liu
  • Jian Ge
  • Meng-xin Wang
  • Lin Cui
  • Bao-yu Han
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 269)


The study was designed to investigate the metabolic kinetics and elimination of Epigallocatechin Gallate (EGCG) in mouse’s tissues, basing on establishing a RP-HPLC method for determination of EGCG in mice plasma, liver, heart, spleen, kidney, pancreas, brain, testis, feces and urine. And the results showed that there was a good linearity over the range 0.1–200.0 mg/L (r > 0.999) for plasma and 0.5–200.0 mg/kg (r > 0.999) for other tissues. The recoveries were more than 85.0 % in all samples, and the intra-and inter-day coefficients of variation were less than 15.0 % in all cases. So the RP-HPLC method was readily applied for pharmacokinetics and elimination of EGCG in mice. EGCG was widely distributed in all the tissues of mice. But it was fast metabolized and eliminated from mice, and the main elimination way was metabolized by hydrolysis enzymes and excreted from urine.


EGCG Elimination Metabolism RP-HPLC 



This work was supported financially by the National Natural Science Foundation of China (31100499 and 31071744) and Natural Science Foundation of Zhejiang Province (Y3080255 and Y3100375). Besides, the article was also funded by Zhejiang Provincial Innovative Research Team Project of University Students (2012R409050).


  1. 1.
    Ge J, Lin F, Li MK et al (2011) Research progress on biological activity of epigallocatechin-3-gallate (EGCG). J Anhui Agric Univ 38(2):156–163Google Scholar
  2. 2.
    Ikuo I, Youji I, Eiji S, et al (1992) Tea catechins decrease micellar solubility and intestinal absorption of cholesterol in rats. Biochimica Biophysica Acta (BBA)-lipids and lipid metabolism 1127(2):141–146Google Scholar
  3. 3.
    Lambert Joshua D, Yang Chung S (2003) Cancer chemopreventive activity and bioavalability of tea and tea polypheols. Mutat Res 523–524:201–208CrossRefGoogle Scholar
  4. 4.
    Elmets CA, Singh D, Tubesing K et al (2001) Cutaneous photoprotection from ultraviolet injury by green tea polyphenols. J Am Acad Dermatol 44:425–432CrossRefGoogle Scholar
  5. 5.
    Williamson MP, McCormick TG, Nance Christina L, et al (2006) Epigallocatechin gallate, the main polyphenoL in green tea, binds to the T-cell receptor, CD4: potential for HIV-therapy. J Allergy Clin Immunol 18(6):1369–1374Google Scholar
  6. 6.
    Lee MJ, Maliakal P, Chen L et al (2002) Pharmacokinetics of tea catechins after ingestion of green tea and (-)-epigallocatechin-3-gallate by humans: formation of different metabolites and individual variability. Cancer Epidemiol Biomark Prev 11:1025–1032Google Scholar
  7. 7.
    Chen L, Lee MJ, Li H et al (1997) Absorption, distribution and elimination of tea polyphenols in rats. Drug Metab Dispos 9:1045–1050Google Scholar
  8. 8.
    Moon YJ, Morris ME (2007) Pharmacokinetics and bioavailability of the bioflavanoid biochanin A: effects of quercetin and EGCG on biochanin A disposition in rats. Mol Pharm 4(6):865–872CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Yang Liu
    • 1
  • Jian Ge
    • 1
  • Meng-xin Wang
    • 1
  • Lin Cui
    • 1
  • Bao-yu Han
    • 2
  1. 1.Department of PharmacyChina Jiliang UniversityHangzhouChina
  2. 2.Zhejiang Provincial Key Laboratory of Biometrology and Inspection and QuarantineHangzhouChina

Personalised recommendations