Research on the Pharmacokinetics and Elimination of Epigallocatechin Gallate (EGCG) in Mice
The study was designed to investigate the metabolic kinetics and elimination of Epigallocatechin Gallate (EGCG) in mouse’s tissues, basing on establishing a RP-HPLC method for determination of EGCG in mice plasma, liver, heart, spleen, kidney, pancreas, brain, testis, feces and urine. And the results showed that there was a good linearity over the range 0.1–200.0 mg/L (r > 0.999) for plasma and 0.5–200.0 mg/kg (r > 0.999) for other tissues. The recoveries were more than 85.0 % in all samples, and the intra-and inter-day coefficients of variation were less than 15.0 % in all cases. So the RP-HPLC method was readily applied for pharmacokinetics and elimination of EGCG in mice. EGCG was widely distributed in all the tissues of mice. But it was fast metabolized and eliminated from mice, and the main elimination way was metabolized by hydrolysis enzymes and excreted from urine.
KeywordsEGCG Elimination Metabolism RP-HPLC
This work was supported financially by the National Natural Science Foundation of China (31100499 and 31071744) and Natural Science Foundation of Zhejiang Province (Y3080255 and Y3100375). Besides, the article was also funded by Zhejiang Provincial Innovative Research Team Project of University Students (2012R409050).
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