Simultaneous Determination of Atractylenolide II and III in Rhizoma Atractylodes Macrocephalae and Chinese Medicinal Preparation by Reverse-Phase High-Performance Liquid Chromatography

Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 269)


This present paper describes the development of a reverse-phase high-performance liquid chromatography (RP-HPLC) coupled with UV spectrometry for simultaneous determination of atractylenolide II and III in traditional Chinese medicines and Chinese medicinal preparation. The standard of atractylenolide II and III was isolated from Rhizoma Atractylodes Macrocephalae. From a variety of solvents tested, methanol was found to be the best solvent for simultaneously extracting atractylenolide II and III from Rhizoma Atractylodes Macrocephalae and Chinese medicinal preparation samples. RP-HPLC analysis of the extracts was performed on an analytical column (SHIMADZU ODS, 150 × 4.6 mm2; i.d., 5 μm) equipped with a security guard pre-column system. The results showed that there was a good linearity over the range 0.5–50.0 μg/ml (r > 0.99). The recoveries were more than 95.0 % in Rhizoma Atractylodes Macrocephalae, and the intra- and inter-day coefficients of variation were less than 5.0 % in all cases. The lower limit of quantification (LLOQ) was 0.5 μg/ml. The RP-HPLC method was readily applied to simultaneously quantitate atractylenolide II and III in Rhizoma Atractylodes Macrocephalae and Chinese medicinal preparation (Shenlingbaizhu granule).


Atractylenolide II Atractylenolide III RP-HPLC Rhizoma Atractylodes macrocephalae 



This work was supported financially by the National Natural Science Foundation of China (31100499) and Zhejiang Provincial Analytical Project (2011C37044). Besides, the article was also funded by Shao-xin Sciences and Technology Project (2011A22009).


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© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Shaoxing University Yuanpei CollegeShaoxingChina
  2. 2.College of Life ScienceChina Jiliang UniversityHangzhouPeople’s Republic of China

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