Genomics of Mineral Nutrient Biofortification: Calcium, Iron and Zinc
Dietary deficiencies affect nearly half of the people on the planet, who simply do not receive sufficient nutrition from the food they buy or grow. Inadequate calcium, iron, and zinc consumption create short and long term health problems, which in turn can magnify and stagnate national development. Dietary diversity, use of industrially fortified foods, and medical interventions are all effective solutions to this suite of related problems. However, each of these solutions requires infrastructure, economic support, and either education or access to markets, and thus are more suitable for the urban than rural poor. Biofortification, or the nutritional enhancement of staple and specialty crops, represents a low cost, sustainable, and potentially effective solution to addressing dietary deficiency and malnutrition in the rural poor. Recent progress on calcium, iron, and zinc biofortification using quantitative genetics, mutational genetics, and genetic engineering technologies will be discussed.
KeywordsQuantitative Trait Locus Iron Deficiency Zinc Deficiency Quantitative Trait Locus Mapping Recombinant Inbred
This work was supported by USDA ARS. The author would like to thank Mrs. Meghan den Bakker and Ms. Ellie Taylor for their excellent work on the research farm during the writing of this review.
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- Assunção AG, Herrero E, Lin YF et al (2010) Arabidopsis thaliana transcription factors bZIP19 and bZIP23 regulate the adaptation to zinc deficiency. Proc Acad Natl Sci U S A 107:10296–10301Google Scholar
- Baxter IR, Gustin JL, Settles AM, Hoekenga OA (2013) Ionomic characterization of maize kernels in the Intermated B73x Mo17 (IBM) population. 53:209–220Google Scholar
- Blair MW, Astudillo C, Grusak MA et al (2009) Inheritance of seed iron and zinc concentrations in common bean (Phaseolus vulgaris L.). Mol Breeding 23:197–207Google Scholar
- Blair MW, Chaves A, Tofino A et al (2010a) Extensive diversity and inter-genepool introgression in a world-wide collection of indeterminate snap bean accessions. Theor Appl Genet 120:1381–1391Google Scholar
- Blair MW, Knewtson SJ, Astudillo C et al (2010b) Variation and inheritance of iron reductase activity in the roots of common bean (Phaseolus vulgaris L.) and association with seed iron accumulation QTL. BMC Plant Biol 10:215Google Scholar
- Blair MW, Medina JI, Astudillo C et al (2010c) QTL for seed iron and zinc concentration and content in a Mesoamerican common bean (Phaseolus vulgaris L.) population. Theor Appl Genet 121:1059–1070Google Scholar
- Bouis HE, Welch R (2010) Biofortification—a sustainable agricultural strategy for reducing micronutrient malnutrition in the global south. Crop Sci 50:20–32Google Scholar
- Cichy KA, Caldas GV, Snapp SS, Blair MW (2009) QTL analysis of seed iron, zinc, and phosphorous levels in an andean bean population. Crop Sci 49:1742–1750Google Scholar
- FAO (2000) Food insecurity: when people live with hunger and fear starvation. The state of food insecurity in the world. FAO, RomeGoogle Scholar
- FAO/WHO (2004a) Calcium. Joint FAO/WHO expert consultation on human vitamin and mineral requirements, 2nd edn. Bangkok, pp 59–93. Accessed from http://whqlibdoc.who.int/publications/2004/9241546123_chap4.pdf. Verified 11/14/13
- FAO/WHO (2004b) Food as a source of nutrients. Joint FAO/WHO expert consultation on human vitamin and mineral requirements, 2nd edn. Bangkok, pp 318–337. Accessed from http://whqlibdoc.who.int/publications/2004/9241546123_chap17.pdf. Verified 11/14/13.
- FAO/WHO (2004c) Iron. Joint FAO/WHO expert consultation on human vitamin and mineral requirements. FAO/WHO, Bangkok, pp 246–278. Accessed from http://whqlibdoc.who.int/publications/2004/9241546123_chap13.pdf. Verified 11/14/13.
- FAO/WHO (2004d) Zinc. Joint FAO/WHO expert consultation on human vitamin and mineral requirements, 2nd edn. Bangkok, pp 230–245 Accessed from http://whqlibdoc.who.int/publications/2004/9241546123_chap12.pdf. Verified 11/14/13.
- Frossard E, Bucher M, Mächler F et al (2000) Potential for increasing the content and bioavailability of Fe, Zn, and Ca in plants for human nutrition. J Sci Food Agric 80:861–879Google Scholar
- Garcia-Oliveira AL, Tan L, Fu Y, Sun C (2009) Genetic identification of quantitative trait loci for contents of mineral nutrients in rice grain. J Intr Plant Bio 51:84–92Google Scholar
- Georges F, Das S, Ray H, Bock C et al (2009) Over-expression of Brassica napus phosphatidylinositol-phospholipase C2 in canola induces significant changes in gene expression and phytohormone distribution patterns, enhances drought tolerance and promotes early flowering and maturation. Plant Cell Environ 32:1664–1681PubMedGoogle Scholar
- Guzman Maldonado H, Martinez O, Acosta GJAetal (2003) Putative quantitiative trait loci for physical and chemical components of common bean. Crop Sci 43:1029–1035Google Scholar
- Hacisalihoglu G, Kochian LV (2003) How do some plants tolerate low levels of soil zinc? Mechanisms of zinc efficiency in crop plants. New Phytol 159:341–350Google Scholar
- Hoekenga O, Gustin J, Flint-Garcia S et al (2010) Ionomics of the maize nested association mapping panel. Vitro Cell Dev Biol-Anim 46:S9–S9Google Scholar
- Hoekenga OA, Lung’aho MG, Tako E et al (2011) Iron biofortification of maize grain. Plant Genet Res 9:327–329Google Scholar
- Horton S, Ross J (2003) The economics of iron deficiency. Food Pol 28:51–75Google Scholar
- Islam FMA, Basford KE, Jara C et al (2002) Seed compositional and disease resistance differences among gene pools in cultivated common bean. Genet Resour Crop Evol 49:285–293Google Scholar
- Khoshgoftarmanesh AH, Sadrarhami A, Sharifi HR et al (2009) Selecting zinc-efficient wheat genotypes with high grain yield using a stress tolerance index. Agron J 101:1409–1416Google Scholar
- Larson SR, Raboy V (1999) Linkage mapping of maize and barley myo-inositol 1-phosphate synthase DNA sequences: correspondence with a low phytic acid mutation. Theor Appl Genet 99:27–36Google Scholar
- Lawrence CJ, Harper LC, Schaeffer ML et al (2008) MaizeGDB: the maize model organism database for basic, translational, and applied research. Int J Plant Genomics 2008:496957. doi: 10.1155/2008/496957.Google Scholar
- Lee S, Jeon US, Lee SJ et al (2009) Iron fortification of rice seeds through activation of the nicotianamine synthase gene. Proc Acad Natl Sci U S A 106:22014–22019Google Scholar
- Lister C, Dean C (1993) Recombinant inbred lines for mapping RFLP and phenotypic markers in Arabidopsis thaliana. Plant J 4:745–750Google Scholar
- Park S, Kim CK, Pike LM et al (2004) Increased calcium in carrots by expression an Arabidopsis H+/Ca2+ transporter. Mol Breeding 14:275–282Google Scholar
- Park S, Cheng NH, Pittman JK et al (2005a) Increased calcium levels and prolonged shelf life in tomatoes expressing Arabidopsis H+/Ca2+ transporters. Plant Physiol 139:1194–1206Google Scholar
- Park S, Kang TS, Kim CK et al (2005b) Genetic manipulation for enhancing calcium content in potato tuber. J Agric Food Chem 53:5598–5603Google Scholar
- Pinstrup-Andersen P (2002) Food and agricultural policy for a globalizing world: preparing for the future. Amer J Agr Econ 84:1201–1214Google Scholar
- Pixley KV, Palacio-Rojas N, Glahn R (2011) The usefulness of iron bioavailability as a target trait for breeding maize (Zea mays L.) with enhanced nutritional value. Field Crops Research 123:153–160Google Scholar
- Pomper KW, Grusak MA (2004) Calcium Uptake and Whole-plant Water Use Influence Pod Calcium Concentration in Snap Bean Plants. J Amer Soc Hort Sci 129:890–895Google Scholar
- Rai KN, Hash CT, Singh AK, Velu G (2008) Adaptation and quality traits of a germplasm-derived commercial seed parent of pearl millet. Plant Genet Res News 154:20–24Google Scholar
- Simic D, Sudar R, Ledencan T et al (2009) Genetic variation of bioavailable iron and zinc in grain of a maize population. J Cereal Sci 50:392–397Google Scholar
- Stangoulis J, Huynh BL, Welch R et al (2007) Quantitative trait loci for phytate in rice grain and their relationship with grain micronutrient content. Euphytica 154:289–294Google Scholar
- Tako E, Blair MW, Glahn RP (2011) Biofortified red mottled beans (Phaseolus vulgaris L.) in a maize and bean diet provide more bioavailable iron than standard red mottled beans: Studies in poultry (Gallus gallus) and in vitro digestion/Caco-2 model. Nutrition J 10:113Google Scholar
- Tako E, Hoekenga OA, Kochian LV, Glahn RP (2013) High bioavailability iron maize (Zea mays L.) developed through molecular breeding provides more bioavailable iron in vitro (Caco-2 model) and in vivo (Gallus gallus). Nutr J 12:3 doi: 10.1186/1475-2891-12-3Google Scholar
- Vreugdenhil D, Aarts MG, Koornneef M et al (2004) Natural variation and QTL analysis for cationic mineral content in seeds of Arabidopsis thaliana. Plant Cell Environ 27:828–839Google Scholar
- Yun S, Habicht J, Miller D, Glahn R (2004) An in vitro digestion/Caco-2 cell culture system accurately predicts the effects of ascorbic acid and polyphenolic compounds on iron bioavailability in humans. J Nutr 134:2712–2721Google Scholar