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Trypanosoma cruzi Trans-Sialidase: Structural Features and Biological Implications

  • Isadora A. Oliveira
  • Leonardo Freire-de-Lima
  • Luciana L. Penha
  • Wagner B. Dias
  • Adriane R. Todeschini
Part of the Subcellular Biochemistry book series (SCBI, volume 74)

Abstract

Trypanosoma cruzi trans-sialidase (TcTS) has intrigued researchers all over the world since it was shown that T. cruzi incorporates sialic acid through a mechanism independent of sialyltransferases. The enzyme has being involved in a vast myriad of functions in the biology of the parasite and in the pathology of Chagas’ disease. At the structural level experiments trapping the intermediate with fluorosugars followed by peptide mapping, X-ray crystallography, molecular modeling and magnetic nuclear resonance have opened up a three-dimensional understanding of the way this enzyme works. Herein we review the multiple biological roles of TcTS and the structural studies that are slowly revealing the secrets underlining an efficient sugar transfer activity rather than simple hydrolysis by TcTS.

Keywords

Sialic Acid Trypanosoma Cruzi Subtelomeric Region Neuraminic Acid Lactobionic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

4-MUNeu5Ac

4-methylumbelliferyl-N-acetyl neuraminic acid

Galf

Galactofuranose

Galp

Galactopyranose

GlcNAc

N-acetylglucosamine

Neu5Ac

N-acetylneuraminic

Neu5Gc

N-glycolylneuraminic acid

NGF

Nerve growth factor receptor

pNPNeu5Ac

p-nitrophenyl-N-acetyl-neuraminic acid

SAPA

Shed acute phase antigen

Sias

Sialic acids

Siglecs

Sia-binding Ig-like lectin

TcTS

Trypanosoma cruzi trans-sialidase

TSs

Trans-sialidase family

UTR

Untranslated regions

Notes

Acknowledgments

The study was financially supported by Conselho Nacional de Desenvolvimento e Tecnologia (CNPq) Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior and the National Institute for Science and Technology in Vaccines (CNPq 573547/2008-4).

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Isadora A. Oliveira
    • 1
  • Leonardo Freire-de-Lima
    • 1
  • Luciana L. Penha
    • 1
  • Wagner B. Dias
    • 1
  • Adriane R. Todeschini
    • 1
  1. 1.Laboratório de Glicobiologia Estrutural e Funcional, Instituto de Biofísica Carlos Chagas FilhoUniversidade Federal do Rio de JaneiroCidade Universitária, Rio de JaneiroBrazil

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