Proteomics Advances in the Study of Leishmania Parasites and Leishmaniasis

  • Jose Batista de Jesus
  • Camila Mesquita-Rodrigues
  • Patricia Cuervo
Part of the Subcellular Biochemistry book series (SCBI, volume 74)


Leishmania spp. are digenetic parasites which cause a broad spectrum of fatal diseases in humans. These parasites, as well as the other trypanosomatid, regulate gene expression at the post-transcriptional and post-translational levels, so that a poor correlation is observed between mRNA content and translated proteins. The completion of the genomic sequencing of several Leishmania species has enormous relevance to the study of the leishmaniasis pathogenesis. The combination of the available genomic resources of these parasites with powerful high-throughput proteomic analysis has shed light on various aspects of Leishmania biology as well as on the mechanisms underlying the disease. Diverse proteomic approaches have been used to describe and catalogue global protein profiles of Leishmania spp., reveal changes in protein expression during development, determine the subcellular localization of gene products, evaluate host-parasite interactions and elucidate drug resistance mechanisms. The characterization of these proteins has advanced, although many fundamental questions remain unanswered. Here, we present a historic review summarizing the different proteomic technologies applied to the study of Leishmania parasites during the last decades and we discuss the proteomic discoveries that have contributed to the understanding of Leishmania parasites biology and leishmaniasis.


Protein Spot Visceral Leishmaniasis Cutaneous Leishmaniasis Resistant Parasite Leishmania Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



One dimensional electrophoresis


Two dimensional differential gel electrophoresis


Two dimensional electrophoresis


Two dimensional liquid chromatography coupled with tandem mass spectrometry


Adenosine triphosphate


Basic local alignment search tool


Deoxyribonucleic acid


Ethylenediamine tetra acetic acid


Electrospray ionization


Electrospray ionization mass spectrometry


Green fluorescent protein


Gene ontology


Glycoprotein 46


Glycoprotein 63 also called leishmanolysin and major surface peptidase


Guanosine triphosphate


Heat shock protein 60


Heat shock protein 70


Heat shock protein 90


Heat shock protein 100


Isoelectric focalization


Immunoglobulin g


Immunoglobulin m


Metal affinity chromatography


Isobaric tags for relative and absolute quantification


Kinetoplastid membrane protein 11


Liquid chromatography coupled with tandem mass spectrometers


Matrix assisted laser desorption ionization


Matrix assisted laser desorption ionization mass spectrometry


Matrix assisted laser desorption ionization tandem mass spectrometry


Messenger ribonucleic acid


Multidimensional protein identification


Post translational modifications


Ras-related protein 7


Ribonucleic acid


Trivalent antimonials


Pentavalent antimonials


Sodium dodecyl sulfate polyacrylamide gel electrophoresis


Small hydrophilic endoplasmic reticulum-associated protein


Stable isotope labeling by amino acids in cell culture




Trichloroacetic acid


T helper 1


World health organization


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Jose Batista de Jesus
    • 1
    • 2
  • Camila Mesquita-Rodrigues
    • 2
  • Patricia Cuervo
    • 3
  1. 1.Departamento de Engenharia de BiossistemasUniversidade Federal de São João Del ReiSão João Del ReiBrazil
  2. 2.Laboratório de Biologia Molecular e Doenças EndêmicasInstituto Oswaldo Cruz, FIOCRUZRio de JaneiroBrazil
  3. 3.Laboratório de Pesquisa em LeishmanioseInstituto Oswaldo Cruz, FIOCRUZRio de JaneiroBrazil

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