Development of Cell-Free Systems

  • Constantin A. Rebeiz
Chapter

Abstract

Originally, work on the biosynthesis of protochlorophyll(ide) [Pchl(ide)] and chlorophyll (Chl) in organello, started in 1967 in my laboratory at the National Research Institute in Tel-el-Amara, Lebanon (see  Chap. 2) (Rebeiz 1967, 1968). At the time spectrophotometric instrumentation was used. Since I was aware that excised etiolated cucumber cotyledons greened very rapidly, within hours, in the light, I conjectured that if greening cotyledons were homogenized, I should be able to observe Pchl(ide) and Chl formation in the homogenate for a few minutes before the system fell apart. The first evidence of Chl biosynthesis in organello was observed in 1967 (Rebeiz 1967). However I soon realized that spectrophotometric techniques were not sensitive enough to observe consistent and reliable Pchl(ide) and Chl biosynthesis in organello. I therefore shifted to the use of 14C-δ-aminolevulinic acid (14C-ALA) as a precursor of 14C-Chl. At the time ALA was known as a tetrapyrrole precursor (Granick 1961). We observed the first incorporation of 14C-ALA into 14C-Chl in my laboratory in 1969. The work was perfected in California at UC Davis in 196–1970 when I joined Paul’s Castelfranco Laboratory (Rebeiz and Castelfranco 1971a, b).

Keywords

Dark Incubation Biosynthetic Activity Crude Homogenate Cucumber Cotyledon Barley Chloroplast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Constantin A. Rebeiz
    • 1
  1. 1.Rebeiz Foundation for Basic ResearchChampaignUSA

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