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The Legionella pneumophila Chaperonin 60 and the Art of Keeping Several Moonlighting Jobs

  • Rafael A. Garduño
  • Audrey Chong
Chapter
Part of the Heat Shock Proteins book series (HESP, volume 7)

Abstract

The title of this chapter intends to emphasize the fact that, as the essential chaperonin 60 of the bacterial pathogen Legionella pneumophila, HtpB has a main day job in protein folding, in addition to several alternate night jobs, depending on where it is located. The alternate virulence-related jobs that we have described for HtpB include the interaction with host cell surface receptors (which results in signaling and a variety of host cell responses), attraction of host cell mitochondria, modification of the host cell actin cytoskeleton, induction of bacterial filamentation, and interaction with specific host cell proteins, i.e. S-adenosyl methionine decarboxylase (which plays a role in the synthesis of host cell polyamines). These alternate HtpB jobs were primarily discovered as strong phenotypes after expression of recombinant HtpB in bacteria, yeast and mammalian cells. It is fascinating that HtpB possesses the extraordinary ability to functionally adapt to the disparate cellular environments of prokaryotic and eukaryotic cells. Although we have not yet been able to decipher the molecular basis for this adaptation, HtpB stands out as an artful moonlighting chaperonin capable of serving several night jobs.

Keywords

Host Cell Cytoplasmic Side Bordetella Pertussis Bacterial Cell Surface Host Cell Cytoplasm 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Department of Microbiology and ImmunologyDalhousie UniversityHalifaxCanada
  2. 2.Department of Medicine – Division of Infectious DiseasesDalhousie UniversityHalifaxCanada
  3. 3.Laboratory of Intracellular ParasitesNIAID, NIH Rocky Mountain LaboratoriesHamiltonUSA

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