Advertisement

Neuroblastoma: Inhibition by Alu-Like RNA

  • Manuele Castelnuovo
  • Tullio Florio
  • Giorgio Dieci
  • Ranieri Cancedda
  • Aldo Pagano
Chapter
Part of the Pediatric Cancer book series (PECA, volume 4)

Abstract

Neuroblastoma (NB) is one of the most frequent pediatric solid tumors characterized by remarkable cell heterogeneity within the cancer nodules. We have recently documented that the synthesis of a pol III-transcribed non-coding (nc) RNA (NDM29) strongly restricts NB development by promoting cell differentiation, a drop of malignancy processes and a dramatic reduction of the tumor initiating cells (TICs) fraction in the NB cell population. Importantly, the overexpression of NDM29 also confers to malignant NB cells an unpredicted susceptibility to the effects of antiblastic drugs used in NB therapy. Thus, treatments able to induce NDM29 expression within cancer cells, making the elusive cancer stem cells more vulnerable to the anticancer drugs, may be used synergistically with traditional cytotoxic therapy to obtain a more complete tumor eradication.

Keywords

Transcription Unit Cancer Nodule HeLa Cell Nuclear Extract Upstream Promoter Element Proximal Sequence Element 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Amid C, Bahr A, Mujica A, Sampson N, Bikar SE, Winterpacht A, Zabel B, Hankeln T, Schmidt ER (2001) Comparative genomic sequencing reveals a strikingly similar architecture of a conserved syntenic region on human chromosome 11p15.3 (including gene ST5) and mouse chromosome 7. Cytogenet Cell Genet 93:284–290PubMedCrossRefGoogle Scholar
  2. Berger A, Strub K (2011) Multiple roles of Alu-related noncoding RNAs. Prog Mol Subcell Biol 51:119–146PubMedCrossRefGoogle Scholar
  3. Brodeur GM (2003) Neuroblastoma: biological insight into a clinical enigma. Nat Rev Cancer 3:203–216PubMedCrossRefGoogle Scholar
  4. Castelnuovo M, Massone S, Tasso R, Fiorino G, Gatti M, Robello M, Gatta E, Berger A, Strub K, Florio T, Dieci G, Cancedda R, Pagano A (2010) An Alu-like RNA promotes cell differentiation and reduces malignancy of human neuroblastoma cells. FASEB J 24(10):4033–4046PubMedCrossRefGoogle Scholar
  5. De Preter K, Vandesompele J, Menten B, Carr P, Fiegler H, Edsjo A, Carter NP, Yigit N, Waelput W, Van Roy N, Bader S, Pahlman S, Speleman F (2005) Positional and functional mapping of a neuroblastoma differentiation gene on chromosome 11. BMC Genomics 6:97PubMedCrossRefGoogle Scholar
  6. Dieci G, Fiorino G, Castelnuovo M, Teichmann M, Pagano A (2007) The expanding RNA polymerase III transcriptome. Trends Genet 23:614–622PubMedCrossRefGoogle Scholar
  7. Dignam JD, Lebovitz RM, Roeder RG (1983) Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res 11:1475–1489PubMedCrossRefGoogle Scholar
  8. Dumay-Odelot H, Durrieu-Gaillard S, Da Silva D, Roeder RG, Teichmann M (2010) Cell growth- and differentiation-dependent regulation of RNA polymerase III transcription. Cell Cycle 9(18):3687–3699, Epub 2010 Sep 1. ReviewPubMedCrossRefGoogle Scholar
  9. George RE, Variend S, Cullinane C, Cotterill SJ, McGuckin AG, Ellershaw C, Lunec J, Pearson AD (2001) Relationship between histopathological ­features, MYCN amplification, and prognosis: a UKCCSG study. United Kingdom Children Cancer Study Group. Med Pediatr Oncol 36:169–176.PubMedCrossRefGoogle Scholar
  10. Jonsson M, Björntorp Mark E, Brantsing C, Brandner JM, Lindahl A, Asp J (2004) Hash4, a novel human achaete-scute homologue found in fetal skin. Genomics 84(5):859–866PubMedCrossRefGoogle Scholar
  11. Massone S, Vassallo I, Fiorino G, Castelnuovo M, Barbieri F, Borghi R, Tabaton M, Robello M, Gatta E, Russo C, Florio T, Dieci G, Cancedda R, Pagano A (2011a) 17A, a novel non-coding RNA, regulates GABA B alternative splicing and signaling in response to inflammatory stimuli and in Alzheimer disease. Neurobiol Dis 41(2):308–317, Epub 2010 Oct 1. (a)PubMedCrossRefGoogle Scholar
  12. Massone S, Vassallo I, Castelnuovo M, Fiorino G, Gatta E, Robello M, Borghi R, Tabaton M, Russo C, Dieci G, Cancedda R, Pagano A (2011b) RNA polymerase III drives alternative splicing of the potassium channel-interacting protein contributing to brain complexity and neurodegeneration. J Cell Biol 193(5):851–866PubMedCrossRefGoogle Scholar
  13. Murphy S, Pierani A, Scheidereit C, Melli M, Roeder RG (1989) Purified octamer binding transcription factors stimulate RNA polymerase III–mediated transcription of the 7SK RNA gene. Cell 59(6):1071–1080PubMedCrossRefGoogle Scholar
  14. Myslinski E, Amé JC, Krol A, Carbon P (2001) An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene. Nucleic Acids Res 29(12):2502–2509. PMID:11410657PubMedCrossRefGoogle Scholar
  15. Orioli A, Pascali C, Pagano A, Teichmann M, Dieci G (2012) RNA polymerase III transcription control elements: themes and variations. Gene 493(2):185–194PubMedCrossRefGoogle Scholar
  16. Pagano A, Castelnuovo M, Tortelli F, Ferrari R, Dieci G, Cancedda R (2007) New small nuclear RNA gene-like transcriptional units as sources of regulatory transcripts. PLoS Genet 3(e1):174–184Google Scholar
  17. Park JR, Eggert A, Caron H (2008) Neuroblastoma: biology, prognosis, and treatment. Pediatr Clin North Am 55:97–120PubMedCrossRefGoogle Scholar
  18. Ross RA, Spengler BA (2007) Human neuroblastoma stem cells. Semin Cancer Biol 17:241–247PubMedCrossRefGoogle Scholar
  19. Ross RA, Spengler BA, Domenech C, Porubcin M, Rettig WJ, Biedler JL (1995) Human neuroblastoma I-type cells are malignant neural crest stem cells. Cell Growth Differ 6:449–456PubMedGoogle Scholar
  20. Walton JD, Kattan DR, Thomas SK, Spengler BA, Guo HF, Biedler JL, Cheung NK, Ross RA (2004) Characteristics of stem cells from human neuroblastoma cell lines and in tumors. Neoplasia 6:838–845PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Manuele Castelnuovo
    • 1
  • Tullio Florio
    • 1
  • Giorgio Dieci
    • 1
  • Ranieri Cancedda
    • 1
  • Aldo Pagano
    • 1
  1. 1.Department of Experimental Medicine (DiMES)University of Genoa and IRCCS-AOU San Martino-ISTGenovaItaly

Personalised recommendations