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Ab Initio Low Resolution Phasing

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Advancing Methods for Biomolecular Crystallography

Abstract

Low resolution ab initio phasing technique may give the first crystallographic images of macromolecules or their complexes when other crystallographic approaches fail to do it due to poor diffraction quality of crystals, in particular those for membrane proteins. This phasing technique uses a set of observed structure factor magnitudes and some general (mathematical) properties of correct low-resolution Fourier syntheses to restore phase values. The paper gives a review of such general properties and discusses the features of their application.

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References

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Acknowledgments

VYL and NLL were supported by RFBR grant 10-04-00254-a. The authors thank M.W. Baumstark, G. Fritz, M.S. Casut, K. Knoops, C. Schaffitzel and J. Steuber for highly fruitful collaboration in study of low resolution structure of Na+-NQR which was describe as an example of phasing in this paper.

Author information

Authors and Affiliations

  1. Institute of Mathematical Problems of Biology, Russian Academy of Sciences, Pushchino, 142290, Russia

    Vladimir Y. Lunin & Natalia L. Lunina

  2. IGBMC, CNRS-INSERM-UdS, 1 rue Laurent Fries, 10142, 67404, Illkirch, France

    Alexandre G. Urzhumtsev

  3. Physics Department, Faculté des Sciences et des Technologies, Université de Lorraine, 239, 54506, Vandoeuvre-lès-Nancy, France

    Alexandre G. Urzhumtsev

Authors
  1. Vladimir Y. Lunin
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  2. Natalia L. Lunina
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  3. Alexandre G. Urzhumtsev
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Corresponding author

Correspondence to Vladimir Y. Lunin .

Editor information

Editors and Affiliations

  1. , Department of Haematology, University of Cambridge, Hills Road, Cambridge, CB2 0XY, United Kingdom

    Randy Read

  2. Fac.des Sciences et des Technologies, Physics Department, Université de Lorraine, Blvd. des Aiguillettes 1, Vandoeuvre-lès-Nancy, 54506, France

    Alexandre G. Urzhumtsev

  3. , Institute of Mathematical Problems of Bi, Russian Academy of Sciences, Institutskaya st. 4, Pushchino, 142290, Russia

    Vladimir Y. Lunin

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Lunin, V.Y., Lunina, N.L., Urzhumtsev, A.G. (2013). Ab Initio Low Resolution Phasing. In: Read, R., Urzhumtsev, A., Lunin, V. (eds) Advancing Methods for Biomolecular Crystallography. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6232-9_17

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