Proteomic Approaches for Urine Biomarker Discovery in Bladder Cancer

  • Ming-Hui Yang
  • Yu-Chang Tyan
Part of the Translational Bioinformatics book series (TRBIO, volume 3)


Bladder cancer is the most common urological cancer with higher incidence rate in the endemic areas of blackfoot disease (BFD) in southern Taiwan. Urine, a blood filtrate produced by the urinary system, is readily collected and is an important source of information for bladder cancers because it is directly exposed to bladder epithelium. Global analysis of the human urinary proteome is important for understanding urinary tract diseases. The aim of this chapter was to utilize the proteomic approach to establish urinary protein patterns of bladder cancer. The experimental results showed that most patients with bladder cancer had proteinuria or albuminuria. In the proteomic analysis, the urinary proteome was identified by nano-high-performance liquid chromatography electrospray ionization tandem mass spectrometry (nano-HPLC-ESI-MS/MS) followed by peptide fragmentation pattern analysis. ADAM28, identified by proteomic approaches and confirmed by ELISA, showed significant differences compared with normal individuals. The upregulation of urinary ADAM28 in bladder cancer was revealed, so it may be a biomarker of bladder cancer.


Proteomics Bladder cancer Urinary protein Protein identification Biomarker 



We are thankful to S. Sheldon MT (ASCP) of Oklahoma University Medical Center Edmond (USA) for fruitful discussions. This work was supported by research grants NSC-099-2811-E-22-002 and NSC-100-2320-B-037-007-MY3 from the National Science Council and NSYSUKMU 101-015 from NSYSU-KMU Joint Research Project, Taiwan, Republic of China.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Department of Chemical and Materials EngineeringNational Yunlin University of Science and TechnologyYunlinTaiwan
  2. 2.Department of Medical Imaging and Radiological Sciences/Center of Excellence for Environmental Medicine/National Sun Yat-Sen University-Kaohsiung Medical University Joint Research CenterKaohsiung Medical UniversityKaohsiungTaiwan

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