The Pathway of Chlorophyll Degradation: Catabolites, Enzymes and Pathway Regulation

Chapter
Part of the Advances in Photosynthesis and Respiration book series (AIPH, volume 36)

Summary

During leaf senescence and fruit ripening, chlorophyll is broken down to colorless linear tetrapyrroles, which are stored in the vacuoles of degreened cells. The pathway of chlorophyll degradation that is active in these developmental processes is fairly well known regarding its biochemistry and cell biology. It comprises at least six enzymatic and one non-enzymatic reaction and the chemical structures of several intermediary and final chlorophyll catabolites have been elucidated. In the last few years, genes coding for a number of chlorophyll catabolic enzymes have been characterized and mutants in these genes have been analyzed. This includes pheophorbide a oxygenase (PAO), the key enzyme of the pathway, which is responsible for opening of the chlorine macrocycle present in chlorophyll, thereby providing the characteristic structural basis of all further downstream breakdown products. The pathway is therefore nowadays termed the ‘PAO pathway’. This review summarizes information on the structures of chlorophyll breakdown products and the reactions involved in their formation. In addition cell biological and regulatory aspects of the PAO pathway are discussed.

Keywords

Leaf Senescence Senescent Leaf Chloroplast Envelope Chlorophyll Breakdown Accelerate Cell Death 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations:

ABC

ATP binding cassette;

ACD

Accelerated cell death;

Chl

Chlorophyll;

Chlide

Chlorophyllide;

CLH

Chlorophyllase;

Fd

Ferre­doxin;

hFCC –

Hypermodified fluorescent Chl catabo­lite;

HMR

Hydroxymethyl Chl reductase;

LHC

Light harvesting complex;

MCS

Metal chelating substance;

mFCC –

Modified fluorescent Chl catabolite;

NCC

Nonfluorescent Chl catabolite;

NOL

NYC1-like;

NYC

Non yellow coloring;

NYE

Non yellowing;

PAO

Pheophorbide a oxygenase;

pFCC

Primary fluorescent Chl catabolite;

Pheide

Pheophorbide;

Phein

Pheophytin;

PPH

Pheophytinase;

PS

Photosystem;

RCC

Red chl catabolite;

RCCR

Red Chl catabolite reductase;

ROS

Reactive oxygen species

Notes

Acknowledgments

I would like to thank Bernhard Kräutler for many stimulating discussions and fruitful long-term collaboration. Many thanks also to my present and former group members for their important contributions in the area of chlorophyll breakdown. I thank Helen Ougham for critical reading and language editing of the manuscript. My work is financially supported by grants from the Swiss National Science Foundation and by the National Center of Competence in Research Plant Survival, a research program of the Swiss National Science Foundation.

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© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Institute of Plant BiologyUniversity of ZurichZurichSwitzerland

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