Protein Kinase C Signaling in Embryonic Stem Cell Self Renewal and Cardiac Differentiation
Embryonic stem cells (ESC); proliferate while maintain the ability to differentiate into several cell types (self-renewal). For the efficient use of ESC in cell therapies it is necessary to characterize the specific signaling pathways that lead to ESC differentiation, proliferation and self-renewal. The Protein kinase C (PKC) family of serine/threonine kinases has been identified as key enzymes for the processes of proliferation and differentiation of ESCs to cardiomyocytes however, the exact function of each PKC isoenzyme remains unclear, and this is partially due to the conserved nature of these kinases and the fact that specific modulators have only recently become available. In the present chapter we discuss recent studies describing the function PKC isoenzymes in murine ESC proliferation, self renewal and cardiac-differentiation.
KeywordsEmbryonic Stem Cell Leukemia Inhibitory Factor Murine Embryonic Stem Cell Cardiomyocyte Differentiation Undifferentiated Embryonic Stem Cell
- Costa-Junior HM, Garavello NM, Duarte ML, Berti DA, Glaser T, Andrade AD, Labate CA, Ferreira AT, Perales JE, Xavier-Neto J, Krieger JE, Schechtman D (2010) Phosphoproteomics profiling suggests a role for nuclear betaIotaPKC in transcription processes of undifferentiated murine embryonic stem cells. J Proteome Res 9:6191–6206PubMedCrossRefGoogle Scholar
- Piazzi M, Bavelloni A, Faenza I, Blalock W, Urbani A, D’aguanno S, Fiume R, Ramazzotti G, Maraldi NM, Cocco L (2010) eEF1A phosphorylation in the nucleus of insulin-stimulated C2C12 myoblasts: Ser(3) is a novel substrate for protein kinase C betaI. Mol Cell Proteomics 9:2719–2728PubMedCrossRefGoogle Scholar