Abstract
Umbilical cord blood (UCB) has become an alternative to bone marrow and peripheral blood as a source of hematopoietic progenitors (HSC) for transplantation. Since the first cord blood transplantation was performed in Paris in 1988, knowledge of biologic characteristics of UCB has greatly improved. UCB banks have greatly facilitated transplantation activity worldwide. The main objective of UCB banks is to select, cryopreserve and store high quality UCB units. Because UCB transplantation may be lifesaving and a particular UCB unit may be the only potential source of HSC for a patient hematopoietic reconstitution, quality of UCB stored in a bank must be strictly assured. Volume reduction and cryopreservation are the standard method for the processing and storage of HSC from UCB intended for transplantation. The main risk in cell cryopreservation is the loss of cell viability. This aspect is especially important for UCB that contains a significant lower HSC content as compared to bone marrow or peripheral blood. The formation and growth of ice crystals and the consequences of osmotic stress on cell membranes can lead to irreversible cell damage. Thus, efforts to avoid it must be implemented in the different phases of cell processing. Dimethyl sulfoxide is the most used cryoprotector for maintaining progenitor cell viability for a long period. In this chapter cryopreservation methodology for UCB banking is exhaustively explained.
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Mirabet, V., Solves, P. (2013). Cryopreservation of Hematopoietic Stem Cells from Umbilical Cord Blood for Transplantation. In: Hayat, M. (eds) Stem Cells and Cancer Stem Cells, Volume 9. Stem Cells and Cancer Stem Cells, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5645-8_1
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DOI: https://doi.org/10.1007/978-94-007-5645-8_1
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