Abstract
Reverse-phase ultra-performance liquid chromatography (RP-UPLC) with online absorbance and fluorescence detection was used for analysis of chernozem soil humic acids (HAs) and their fractions A, B, and C + D with different electrophoretic mobility (EM) and molecular size (MS). A strong relationship between fluorescence properties, EM, MS, polarity, and aliphaticity/aromaticity of HAs fractions was found. Humic and protein-like fluorescence had different polarity nature. Protein-like fluorescence is located in humic material which irreversibly adsorbed on the reversed-phase column and was not subjected to RP-UPLC characterization. The humic-like fluorescence is mostly located in the hydrophilic part of fraction C + D. Taking into account that high MS fraction A consists mainly of aliphatic components, it is reasonable to suggest that these associations are capable of organizing into micellar structures.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Trubetskoj, O.A., O.E. Trubetskaya, G.V. Afanas’eva, O.I. Reznikova, and C. Saiz-Jimenez. 1997. Polyacrylamide gel electrophoresis of soil humic acid fractionated by size-exclusion chromatography and ultrafiltration. Journal of Chromatography A 767: 285–292.
Trubetskaya, O.E., O.A. Trubetskoj, G.V. Afanas’eva, O.I. Reznikova, L.F. Markova, and T.A. Muranova. 1998. Amino acid distribution in soil humic acids fractionated by tandem size exclusion chromatography – Polyacrylamide gel electrophoresis. Environment International 24: 573–581.
Trubetskoj, O.A., P.G. Hatcher, and O.E. Trubetskaya. 2010. 1H-NMR and 13C-NMR spectroscopy of chernozem soil humic acid fractionated by combined size-exclusion chromatography and electrophoresis. Chemistry and Ecology 26: 315–325.
Acknowledgements
The work has been supported by Russian Foundation for Basic Research (project 10-05-00243-a) and bilateral programme CNRS-RAS (project no. 23962).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Zhejiang University Press and Springer Science+Business Media Dordrecht
About this paper
Cite this paper
Trubetskaya, O., Richard, C., Voyard, G., Trubetskoj, O. (2013). Relationships Between Polarity, Aliphaticity/Aromaticity, Fluorescence, and Molecular Size of Soil HA Electrophoretic Fractions. In: Xu, J., Wu, J., He, Y. (eds) Functions of Natural Organic Matter in Changing Environment. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5634-2_34
Download citation
DOI: https://doi.org/10.1007/978-94-007-5634-2_34
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-007-5633-5
Online ISBN: 978-94-007-5634-2
eBook Packages: Earth and Environmental ScienceEarth and Environmental Science (R0)