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Integrated Physical-Chemical Procedure for Soil Organic Carbon Fractionation and Characterization During Transition to Organic Farming

  • H. M. AbdelrahmanEmail author
  • D. C. Olk
  • C. Cocozza
  • D. Ventrella
  • F. Montemurro
  • T. Miano
Conference paper
  • 332 Downloads

Abstract

Two field experiments, in the south of Italy, were established in 2009 to study and characterize soil organic matter (SOM) during transition to organic farming. Experiments included a cereal/leguminous rotation fertilized with permitted amendments with three field replicates. A sequential fractionation procedure was used to separate different SOM fractions: light fraction (LF), two size classes of particulate organic matter (POM), mobile humic acid (MHA), and Ca-bound humic acid (CaHA). Isolated fractions were quantified and analyzed for their C and N content and carbohydrate and amino compound composition. The masses of the isolated fractions increased during 2-year course, with noticeable increases in LF and POM. Moreover, LF and POM were found more responsive than MHA to treatment and crop. The xylose/mannose ratio explained that MHA-carbohydrates were mainly of microbial origin, while LF- and POM-carbohydrates were of plant origin. Amino compounds constituted up to 30% of total soil N and were found to be more responsive to seasonal variation than to agronomic practices.

Keywords

Mobile humic acid (MHA) Light fraction Labile organic carbon Particulate organic matter Amino acids Carbohydrates 

References

  1. Cao, X., D.C. Olk, M. Chappell, C.A. Cambardella, L.F. Miller, and J. Mao. 2011. Solid-state NMR analysis of soil organic matter fractions from integrated physical–chemical extraction. Soil Science Society of America Journal 75: 1374–1384.CrossRefGoogle Scholar
  2. Martens, D.A., and K.L. Loeffelmann. 2002. Improved accounting of carbohydrate carbon from plants and soils. Soil Biology and Biochemistry 34: 1393–1399.CrossRefGoogle Scholar
  3. Martens, D.A., and K.L. Loeffelmann. 2003. Soil amino acid composition quantified by acid hydrolysis and anion chromatography-pulsed amperometry. Journal of Agricultural and Food Chemistry 51: 6521–6522.CrossRefGoogle Scholar

Copyright information

© Zhejiang University Press and Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • H. M. Abdelrahman
    • 1
    • 2
    Email author
  • D. C. Olk
    • 2
  • C. Cocozza
    • 1
  • D. Ventrella
    • 3
  • F. Montemurro
    • 4
  • T. Miano
    • 1
  1. 1.Department of Biology and Chemistry of Agro-Forestry and EnvironmentUniversity of BariBariItaly
  2. 2.USDA-ARS – National Laboratory for Agriculture and the EnvironmentAmesUSA
  3. 3.Research Unit for Cropping Systems in Dry Environments (CRA-SCA)BariItaly
  4. 4.Research Unit for the Study of Cropping Systems (CRA-SSC)MetapontoItaly

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