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Proteins and Powders: An Overview

  • Irene Margiolaki
Conference paper
Part of the NATO Science for Peace and Security Series B: Physics and Biophysics book series (NAPSB)

Abstract

Following the seminal work of Von Dreele, powder X-ray diffraction studies on proteins are being established as a valuable complementary technique to single crystal measurements. A wide range of small proteins have been found to give synchrotron powder diffraction profiles where the peak widths are essentially limited only by the instrumental resolution. The rich information contained in these profiles, combined with developments in data analysis, has stimulated research and development to apply the powder technique to microcrystalline protein samples. In this chapter, progress in using powder diffraction for macromolecular crystallography is reported.

Keywords

Powder Diffraction Data Molecular Replacement Purple Membrane Powder Data Macro Domain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

I am grateful to Dr. A.N. Fitch & Dr. J.P. Wright for their continuous input, advice and support during this project. I would like to thank six undergraduate students from the University of Bath who have worked with us 1 year each: S. Basso, L. Knight, S. Dagogo, M. Jenner, I. Collings, L. Saunders as well as our PhD student, Y. Watier. Many thanks to my three M.Sc. students at the department of biology of the University of Patras (UPAT): Miss Evdokia-Anastasia Giannopoulou, Foteini Karavasili & Eleni Kotsiliti. Special thanks go to Drs. R.B. Von Dreele, M. Norrman, G. Schluckebier, M. El Hajji, F. Bonnete, M. Giffard, N. Papageorgiou, B. Canard, B. Coutard, M. Wilmanns and N. Pinotsis, and also Dr. M. Schiltz and his group, for the provision of interesting samples, their help and collaboration. I am grateful to the UNESCO and L’OREAL foundations for the international fellowship for women in science (2010). Finally, we thank the ESRF for provision of synchrotron beam time and UPAT for provision of facilities and financial resources.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  1. 1.Department of Biology, Section of Genetics, Cell Biology and DevelopmentUniversity of PatrasPatrasGreece

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