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Proteins and Powders: An Overview

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Uniting Electron Crystallography and Powder Diffraction

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Abstract

Following the seminal work of Von Dreele, powder X-ray diffraction studies on proteins are being established as a valuable complementary technique to single crystal measurements. A wide range of small proteins have been found to give synchrotron powder diffraction profiles where the peak widths are essentially limited only by the instrumental resolution. The rich information contained in these profiles, combined with developments in data analysis, has stimulated research and development to apply the powder technique to microcrystalline protein samples. In this chapter, progress in using powder diffraction for macromolecular crystallography is reported.

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Acknowledgments

I am grateful to Dr. A.N. Fitch & Dr. J.P. Wright for their continuous input, advice and support during this project. I would like to thank six undergraduate students from the University of Bath who have worked with us 1 year each: S. Basso, L. Knight, S. Dagogo, M. Jenner, I. Collings, L. Saunders as well as our PhD student, Y. Watier. Many thanks to my three M.Sc. students at the department of biology of the University of Patras (UPAT): Miss Evdokia-Anastasia Giannopoulou, Foteini Karavasili & Eleni Kotsiliti. Special thanks go to Drs. R.B. Von Dreele, M. Norrman, G. Schluckebier, M. El Hajji, F. Bonnete, M. Giffard, N. Papageorgiou, B. Canard, B. Coutard, M. Wilmanns and N. Pinotsis, and also Dr. M. Schiltz and his group, for the provision of interesting samples, their help and collaboration. I am grateful to the UNESCO and L’OREAL foundations for the international fellowship for women in science (2010). Finally, we thank the ESRF for provision of synchrotron beam time and UPAT for provision of facilities and financial resources.

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Authors and Affiliations

  1. Department of Biology, Section of Genetics, Cell Biology and Development, University of Patras, GR-26500, Patras, Greece

    Irene Margiolaki

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  1. Irene Margiolaki
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Correspondence to Irene Margiolaki .

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Editors and Affiliations

  1. , Institute of Physical Chemistry, Johannes Gutenberg University, Welderweg 11, Mainz, 55128, Germany

    Ute Kolb

  2. , School of Pharmacy, University of Reading, Whiteknights, Reading, RG6 6AP, United Kingdom

    Kenneth Shankland

  3. , Department of Materials Engineering, Ben-Gurion University of the Negev, Marcus Family Campus, Beer-Sheva, 84105, Israel

    Louisa Meshi

  4. Inst. Crystallography, Russian Academy of Sciences, Leninskii prospect 59, Moscow, 119333, Russia

    Anatoly Avilov

  5. , Isis Facility, STFC Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, OX11 0QX, United Kingdom

    William I.F David

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Margiolaki, I. (2012). Proteins and Powders: An Overview. In: Kolb, U., Shankland, K., Meshi, L., Avilov, A., David, W. (eds) Uniting Electron Crystallography and Powder Diffraction. NATO Science for Peace and Security Series B: Physics and Biophysics. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5580-2_13

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  • DOI: https://doi.org/10.1007/978-94-007-5580-2_13

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  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-007-5579-6

  • Online ISBN: 978-94-007-5580-2

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