Approaches Toward the Study of Halophilic Microorganisms in Their Natural Environments: Who Are They and What Are They Doing?

  • Aharon Oren


Hypersaline lakes with salt concentrations exceeding 250 g/l are often characterized by very dense communities of halophilic microorganisms imparting a red coloration to the brines. Such red waters can be found in the North Arm of Great Salt Lake , Utah, in crystallizer ponds of solar salterns for the production of salt from seawater, and in many extremely hypersaline alkaline lakes. At times even the magnesium chloride-rich waters of the Dead Sea have become red as a result of massive development of pigmented salt-loving microorganisms.


Great Salt Lake Hypersaline Environment Osmotic Solute Mono Lake Crystallizer Pond 
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  1. Antón J, Llobet-Brossa E, Rodríguez-Valera F, Amann R (1999) Fluorescence in situ hybridization analysis of the prokaryotic community inhabiting crystallizer ponds. Environ Microbiol 1:517–523PubMedCrossRefGoogle Scholar
  2. Antón J, Rosselló-Mora R, Rodríguez-Valera F, Amann R (2000) Extremely halophilic Bacteria in crystallizer ponds from solar salterns. Appl Environ Microbiol 66:3052–3057PubMedCrossRefGoogle Scholar
  3. Antón J, Oren A, Benlloch S, Rodríguez-Valera F, Amann R, Rosselló-Mora R (2002) Salinibacter ruber gen. nov., sp. nov., a novel extreme halophilic member of the Bacteria from saltern crystallizer ponds. Int J Syst Evol Microbiol 52:485–491PubMedGoogle Scholar
  4. Antón J, Peña A, Santos F, Martínez-García M, Schmitt-Kopplin P, Rosselló-Mora R (2008) Distribution, abundance and diversity of the extremely halophilic bacterium Salinibacter ruber. Sal Syst 4:15CrossRefGoogle Scholar
  5. Baati H, Guermazi S, Amdouni R, Gharsallah N, Sghir A, Ammar E (2008) Prokaryotic diversity of a Tunisian multipond solar saltern. Extremophiles 12:505–518PubMedCrossRefGoogle Scholar
  6. Baati H, Guermazi S, Gharsallah N, Sghir A, Ammar E (2010) Novel prokaryotic diversity in sediments of Tunisian multipond solar saltern. Res Microbiol 161:573–582PubMedCrossRefGoogle Scholar
  7. Benlloch S, Martínez-Murcia AJ, Rodríguez-Valera F (1995) Sequencing of bacterial and archaeal 16S rRNA genes directly amplified from a hypersaline environment. Syst Appl Microbiol 18:574–581.CrossRefGoogle Scholar
  8. Benlloch S, Acinas SG, Martínez-Murcia AJ, Rodríguez-Valera F (1996) Description of prokaryotic biodiversity along the salinity gradient of a multipond saltern by direct PCR amplification of 16S rDNA. Hydrobiologia 329:19–31CrossRefGoogle Scholar
  9. Benlloch S, López-López A, Casamayor EO, Øvreås L, Goddard V, Dane FL, Smerdon G, Massana R, Joint I, Thingstad F, Pedrós-Alió C, Rodríguez-Valera F (2002) Prokaryotic genetic diversity throughout the salinity gradient of a coastal solar saltern. Environ Microbiol 4:349–360PubMedCrossRefGoogle Scholar
  10. Brandt KK, Vester F, Jensen AN, Ingvorsen K (2001) Sulfate reduction dynamics and enumeration of sulfate-reducing bacteria in hypersaline sediments of the Great Salt Lake (Utah, USA). Microb Ecol 41:1–11PubMedGoogle Scholar
  11. Brum JR, Steward GF (2010) Morphological characterization of viruses in the stratified water column of alkaline, hypersaline Mono Lake. Microb Ecol 60:636–643PubMedCrossRefGoogle Scholar
  12. Burns DG, Camakaris HM, Janssen PH, Dyall-Smith ML (2004) Combined use of cultivation-dependent and cultivation-independent methods indicates that members of most haloarchaeal groups in an Australian crystallizer pond are cultivable. Appl Environ Microbiol 70:5258–5265PubMedCrossRefGoogle Scholar
  13. Burns DG, Janssen PH, Itoh T, Kamekura M, Li Z, Jensen G, Rodríguez-Valera F, Bolhuis H, Dyall-Smith ML (2007) Haloquadratum walsbyi gen. nov., sp. nov., the square haloarchaeon of Walsby, isolated from saltern crystallizers in Australia and Spain. Int J Syst Evol Microbiol 57:387–392PubMedCrossRefGoogle Scholar
  14. Canfield DE, Sørensen KB, Oren A (2004) Biogeochemistry of a gypsum-encrusted microbial ecosystem. Geobiology 2:133–150CrossRefGoogle Scholar
  15. Casamayor EO, Massana R, Benlloch S, Øvreås L, Díez B, Goddard VJ, Gasol JM, Joint I, Rodríguez-Valera F, Pedrós-Alió C (2002) Changes in archaeal, bacterial and eukaryal assemblages along a salinity gradient by comparison of genetic fingerprinting methods in a multipond solar saltern. Environ Microbiol 4:338–348PubMedCrossRefGoogle Scholar
  16. Corcelli A, Lobasso S (2006) Characterization of lipids of halophilic Archaea. In: Rainey FA, Oren A (eds) Extremophiles—Methods in microbiology, vol. 35. Elsevier/Academic Press, Amsterdam.Google Scholar
  17. Corcelli A, Lattanzio VMT, Mascolo G, Babudri F, Oren A, Kates M (2004) Novel sulfonolipid in the extremely halophilic bacterium Salinibacter ruber. Appl Environ Microbiol 70:6678–6685PubMedCrossRefGoogle Scholar
  18. Demergasso C, Escudero L, Casamayor EO, Chong G, Balagué V, Pedrós-Alió C (2008) Novelty and spatio-temporal heterogeneity in the bacterial diversity of hypersaline Lake Tebenquiche (Salar de Atacama). Extremophiles 12:491–504PubMedCrossRefGoogle Scholar
  19. Diez B, Antón J, Guixa-Boixereu N, Pedrós-Alió C, Rodríguez-Valera F (2000) Pulsed-field gel electrophoresis analysis of virus assemblages present in a hypersaline environment. Int Microbiol 3:159–164PubMedGoogle Scholar
  20. Dussault HP (1956) Study of red halophilic bacteria in solar salt and salted fish: II. Bacto-oxgall as a selective agent for differentiation. J Fish Res Bd Canada 13:195–199CrossRefGoogle Scholar
  21. Eder W, Ludwig W, Huber R (1999) Novel 16S rRNA gene sequences retrieved from highly saline brine sediments of Kebrit Deep, Red Sea. Arch Microbiol 172:213-218PubMedCrossRefGoogle Scholar
  22. Eder W, Jahnke LL, Schmidt M, Huber R (2001) Microbial diversity of the brine-seawater interface of the Kebrit Deep, Red Sea, studied via 16S rRNA gene sequences and cultivation methods. Appl Environ Microbiol 67:3077–3085PubMedCrossRefGoogle Scholar
  23. Eder W, Schmidt M, Koch M, Garbe-Schönberg D, Huber R (2002) Prokaryotic phylogenetic diversity and corresponding geochemical data of the brine-seawater interface of the Shaban Deep, Red Sea. Environ Microbiol 4:758–763PubMedCrossRefGoogle Scholar
  24. Elevi Bardavid R, Oren A (2008a) Dihydroxyacetone metabolism in Salinibacter ruber and in Haloquadratum walsbyi. Extremophiles 12:125–131CrossRefGoogle Scholar
  25. Elevi Bardavid R, Oren A (2008b) Sensitivity of Haloquadratum and Salinibacter to antibiotics and other inhibitors: implications for the assessment of the contribution of Archaea and Bacteria to heterotrophic activities in hypersaline environments. FEMS Microbiol Ecol 63:309–315CrossRefGoogle Scholar
  26. Elevi Bardavid R, Oren A (2012) Acid-shifted isoelectric point profiles of the proteins in a hypersaline microbial mat—an adaptation to life at high salt concentrations? Extremophiles 16:787–792Google Scholar
  27. Elevi Bardavid R, Khristo P, Oren A (2008) Interrelationships between Dunaliella and halophilic prokaryotes in saltern crystallizer ponds. Extremophiles 12:5–14CrossRefGoogle Scholar
  28. Estrada M, Henriksen P, Gasol JM, Casamayor EO, Pedrós-Alió C (2004) Diversity of planktonic photoautotrophic microorganisms along a salinity gradients as depicted by microscopy, flow cytometry, pigment analysis and DNA-based methods. FEMS Microbiol Ecol 49:281–293PubMedCrossRefGoogle Scholar
  29. Ferrer M, Werner J, Chernikova TN, Bargiela R, Fernández L, La Cono V, Waldmann J, Teeling H, Golyshina OV, Glöckner FO, Yakimov MM, Golyshin PN, the MAMBA Scientific Consortium (2012) Unveiling microbial life in the new deep-sea hypersaline Lake Thetis. Part II: a metagenomic study. Environ Microbiol 14:268–281.PubMedCrossRefGoogle Scholar
  30. Garcia-Heredia I, Martin-Cuadrado A-B, Mojica FJM, Santos F, Mira A, Antón J, Rodríguez-Valera F (2012) Reconstructing viral genomes from the environment using fosmid clones: the case of haloviruses. PLoS One 7:e33802CrossRefGoogle Scholar
  31. Gareeb AP, Setati ME (2009) Assessment of alkaliphilic haloarchaeal diversity in Sua pan evaporator ponds in Botswana. Afr J Biotechnol 8:259–267Google Scholar
  32. Gasol JM, Casamayor EO, Joint I, Garde K, Gustavson K, Benlloch S, Díez B, Schauer M, Massana R, Pedrós-Alió C (2004) Control of heterotrophic prokaryotic abundance and growth rate in hypersaline planktonic environments. Aquat Microb Ecol 34:193–206.CrossRefGoogle Scholar
  33. Ghai R, Fernández AB, Martin-Cuadrado A-B, Megumi Mizuno C, McMahon KD, Papke RT, Stepanauskas R, Rodriguez-Brito B, Rohwer F, Sánchez-Porro C, Ventosa A, Rodríguez-Valera F (2011) New abundant microbial groups in aquatic hypersaline environments. Sci Rep 1:135.PubMedCrossRefGoogle Scholar
  34. Giri BJ, Bano N, Hollibaugh JT (2004) Distribution of RuBisCO genotypes along a redox gradient in Mono Lake, California. Appl Environ Microbiol 70:3443–3448.Google Scholar
  35. Grant S, Grant WD, Jones BE, Kato C, Li L (1999) Novel archaeal phylotypes from an East African alkaline saltern. Extremophiles 3:139–145.PubMedCrossRefGoogle Scholar
  36. Guixa-Boixareu N, Calderón-Paz JI, Heldal M, Bratbak G, Pedrós-Alió C (1996) Viral lysis and bacterivory as prokaryotic loss factors along a salinity gradient. Aquat Microb Ecol 11;215–227.CrossRefGoogle Scholar
  37. Gunde-Cimerman N, Zalar P, de Hoog GS, Plemenitaš A (2000) Hypersaline water in salterns—natural ecological niches for halophilic black yeasts. FEMS Microbiol Ecol 32:235–240.Google Scholar
  38. Humayoun SB, Bano N, Hollibaugh JT (2003) Depth distribution of microbial diversity in Mono Lake, a meromictic soda lake in California. Appl Environ Microbiol 69:1030–1042.Google Scholar
  39. Ionescu D, Lipski A, Altendorf K, Oren A (2007) Characterization of the endoevaporitic microbial communities in a hypersaline gypsum crust by fatty acid analysis. Hydrobiologia 576:15–26.CrossRefGoogle Scholar
  40. Javor BJ (1983) Planktonic standing crop and nutrients in a saltern ecosystem. Limnol Oceanogr 28:153–159.CrossRefGoogle Scholar
  41. Javor B (1989) Hypersaline environments. Microbiology and biogeochemistry. Springer-Verlag, Berlin.Google Scholar
  42. Jiang S, Steward G, Jellison R, Chu W, Choi S (2004) Abundance, distribution, and diversity of viruses in alkaline, hypersaline Mono Lake, California. Microb Ecol 47:9–17.Google Scholar
  43. Joint I, Henriksen P, Garde K, Riemann B (2002) Primary production, nutrient assimilation and microzooplankton grazing along a hypersaline gradient. FEMS Microbiol Ecol 39:245–257.PubMedCrossRefGoogle Scholar
  44. Joye SB, Samarkin VA, Orcutt BM, MacDonald IR, Hinrichs K-U, Elvert M, Teske AP, Lloyd KG, Lever MA, Montoya JP, Meile CD (2009) Metabolic variability in seafloor brines revealed by carbon and sulphur dynamics. Nature Geosci 2:349–354.CrossRefGoogle Scholar
  45. Kamekura M, Oesterhelt D, Wallace R, Anderson P, Kushner DJ (1988) Lysis of halobacteria in Bacto-peptone by bile acids. Appl Environ Microbiol 54:990–995.PubMedGoogle Scholar
  46. Kis-Papo T, Oren A (2000) Halocins: are they important in the competition between different types of halobacteria in saltern ponds? Extremophiles 4:35–41.PubMedGoogle Scholar
  47. Kjeldsen KU, Loy A, Jakobsen TF, Thomsen TR, Wagner M, Ingvorsen K (2006) Diversity of sulfate-reducing bacteria from an extreme hypersaline sediment, Great Salt Lake (Utah). FEMS Microbiol Ecol 60:287–298.CrossRefGoogle Scholar
  48. Kunin V, Raes J, Harris JK, Spear JR, Walker JJ, Ivanova N, von Mering C, Bebout BM, Pace NR, Bork P, Hugenholtz P (2008) Millimeter scale genetic gradients and community-level molecular convergence in a hypersaline microbial mat. Mol Systems Biol 4:198.Google Scholar
  49. La Cono V, Smedile F, Bortoluzzi G, Arcadi E, Maimone G, Messina E, Borghini M, Oliveri E, Mazzola S, L’Haridon S, Toffin L, Genovese L, Ferrer M, Giuliano L, Golyshin PN, Yakimov MM (2011) Unveiling microbial life in new deep-sea hypersaline Lake Thetis. Part I: Prokaryotes and environmental settings. Environ Microbiol 13:2250–2268.PubMedCrossRefGoogle Scholar
  50. Lattanzio V, Corcelli A, Mascolo G, Oren A (2002) Presence of two novel cardiolipins in the halophilic archaeal community in the crystallizer brines from the salterns of Margherita di Savoia (Italy) and Eilat (Israel). Extremophiles 6:437–444.PubMedCrossRefGoogle Scholar
  51. Legault BA, Lopez-Lopez A, Alba-Casado JC, Doolittle WF, Bolhuis H, Rodríguez-Valera F, Papke RT (2006) Environmental genomics of "Haloquadratum walsbyi" in a saltern crystallizer indicates a large pool of accessory genes in an otherwise coherent species. BMC Genomics 7:171.PubMedCrossRefGoogle Scholar
  52. Leuko S, Legat A, Fendrihan S, Stan-Lotter H (2004) Evaluation of the LIVE/DEAD BacLight kit for detection of extremophilic Archaea and visualization of microorganisms in environmental hypersaline samples. Appl Environ Microbiol 70:6884–6886.PubMedCrossRefGoogle Scholar
  53. Leuko S, Goh F, Allen MA, Burns BP, Walter MR, Neilan BA (2007) Analysis of intergenic spacer region length polymorphisms to investigate the halophilic archaeal diversity of stromatolites and microbial mats. Extremophiles 11:203–210.PubMedCrossRefGoogle Scholar
  54. Leuko S, Goh F, Ibáñez-Peral R, Burns BP, Walker MR, Neilan BA (2008) Lysis efficiency of standard DNA extraction methods for Halococcus spp. in an organic rich environment. Extremophiles 12:301-308.PubMedCrossRefGoogle Scholar
  55. Litchfield CD, Gillivet PM (2002) Microbial diversity and complexity in hypersaline environments: a preliminary assessment. J Ind Microbiol Biotchnol 28:48–55.Google Scholar
  56. Litchfield CD, Oren A (2001) Polar lipids and pigments as biomarkers for the study of the microbial community structure of solar salterns. Hydrobiologia 466:81–89.CrossRefGoogle Scholar
  57. Litchfield CD, Irby A, Kis-Papo T, Oren A (2000) Comparisons of the polar lipid and pigment profiles of two solar salterns located in Newark, California, USA, and Eilat, Israel. Extremophiles 4:259–265.PubMedCrossRefGoogle Scholar
  58. Litchfield CD, Irby A, Kis-Papo T, Oren A (2001) Comparative metabolic diversity in two solar salterns. Hydrobiologia 466:73–80.CrossRefGoogle Scholar
  59. Litchfield CD, Sikaroodi M, Gillivet PM (2006) Characterization of natural communities of halophilic microorganisms. In: Rainey FA, Oren A (eds), Extremophiles—Methods in microbiology, vol. 35. Elsevier/Academic Press, Amsterdam.Google Scholar
  60. Lobasso S, Lopalco P, Mascolo G, Corcelli A (2008) Lipids of the ultra-thin square halophilic archaeon Haloquadratum walsbyi. Archaea 2:177–181.PubMedCrossRefGoogle Scholar
  61. Lopalco P, Lobasso S, Baronio M, Angelini R, Corcelli A (2011) Impact of lipidomics on the microbial world of hypersaline environments. In: Ventosa A, Oren A, Ma Y (eds), Halophiles and hypersaline environments. Current research and future trends. Springer, Heidelberg.Google Scholar
  62. Lutnæs BF, Oren A, Liaaen-Jensen S (2002) New C40-carotenoid acyl glycoside as principal carotenoid of Salinibacter ruber, an extremely halophilic eubacterium. J Nat Prod 65:1340–1343.PubMedCrossRefGoogle Scholar
  63. Ma Y, Zhang W, Xue Y, Zhou P, Ventosa A, Grant WD (2004) Bacterial diversity of the Inner Mongolian Baer Soda Lake as revealed by 16S rRNA gene sequence analyses. Extremophiles 8:45–51.PubMedCrossRefGoogle Scholar
  64. Makhdoumi-Kakhki A, Amoozegar MA, Kazemi B, Pašić L, Ventosa A (2012) Prokaryotic diversity in Aran-Bidgol salt lake, the largest hypersaline playa in Iran. Microbes Environ 27:87–93.PubMedCrossRefGoogle Scholar
  65. Manikandan M, Kannan V, Pašić L (2009) Diversity of microorganisms in solar salterns of Tamil Nadu, India. World J Microbiol Biotechnol 25:1007–1017.CrossRefGoogle Scholar
  66. Maturrano L, Santos F, Rosselló-Mora R, Antón J (2006) Microbial diversity in Maras salterns, a hypersaline environment in the Peruvian Andes. Appl Environ Microbiol 72:3887–3895.PubMedCrossRefGoogle Scholar
  67. Mesbah NM, Abou-El-Ela SH, Wiegel J (2007) Novel and unexpected prokaryotic diversity in water and sediments of the alkaline, hypersaline lakes of the Wadi An Natrun, Egypt. Microb Ecol 54:598–617.PubMedCrossRefGoogle Scholar
  68. Mouné S, Caumette P, Matheron R, Willison JC (2002) Molecular sequence analysis of prokaryotic diversity in the anoxic sediments underlying cyanobacterial mats of two hypersaline ponds in Mediterranean salterns. FEMS Microbiol Ecol 44:117–130.CrossRefGoogle Scholar
  69. Mwrichia R, Cousin S, Muigai AW, Boga HI, Stackebrandt E (2010) Archaeal diversity in the haloalkaline Lake Elmenteita in Kenya. Curr Microbiol 60:47–52.CrossRefGoogle Scholar
  70. Narasingarao P, Podell S, Ugalde JA, Brochier-Armanet C, Emerson JB, Brocks JJ, Heidelberg KB, Banfield JF, Allen EE (2012) De novo assembly reveals abundant novel major lineage of Archaea in hypersaline microbial communities. ISME J 6:81–93.PubMedCrossRefGoogle Scholar
  71. Nissenbaum A, Kaplan IR (1976) Sulfur and carbon isotopic evidence for biogeochemical processes in the Dead Sea. In: Nriagu JO (ed), Environmental biogeochemistry, vol. 1. Ann Arbor Science Publishers, Ann Arbor.Google Scholar
  72. Ochsenreiter T, Pfeifer F, Schleper C (2002) Diversity of Archaea in hypersaline environments characterized by molecular-phylogenetic and cultivation studies. Extremophiles 6:267–274.PubMedCrossRefGoogle Scholar
  73. Oremland RS, King GM (1989) Methanogenesis in hypersaline environments. In: Cohen Y, Rosenberg E (eds), Microbial mats. Physiological ecology of benthic microbial communities. American Society for Microbiology, Washington, DC.Google Scholar
  74. Oren A (1983a) Population dynamics of halobacteria in the Dead Sea water column. Limnol Oceanogr 28:1094–1103.CrossRefGoogle Scholar
  75. Oren A (1983b) Bacteriorhodopsin-mediated CO2 photoassimilation in the Dead Sea. Limnol Oceanogr 28:33–41.CrossRefGoogle Scholar
  76. Oren A (1989) A method for the differential microscopic enumeration of Halobacterium cells. J Microbiol Meth 10:183–187.CrossRefGoogle Scholar
  77. Oren A (1990a) Formation and breakdown of glycine betaine and trimethylamine in hypersaline environments. Antonie van Leeuwenhoek 58:291–298.CrossRefGoogle Scholar
  78. Oren A (1990b) Thymidine incorporation in saltern ponds of different salinities: estimation of in situ growth rates of halophilic archaeobacteria and eubacteria. Microb Ecol 19:43–51.CrossRefGoogle Scholar
  79. Oren A (1990c) The use of protein synthesis inhibitors in the estimation of the contribution of halophilic archaebacteria to bacterial activity in hypersaline environments. FEMS Microbiol Ecol 73:187–192.CrossRefGoogle Scholar
  80. Oren A (1990d) Estimation of the contribution of halobacteria to the bacterial biomass and activity in a solar saltern by the use of bile salts. FEMS Microbiol Ecol 73:41–48.CrossRefGoogle Scholar
  81. Oren A (1992) Bacterial activities in the Dead Sea, 1980–1991: survival at the upper limit of salinity. Int J Salt Lake Res 1:7–20.CrossRefGoogle Scholar
  82. Oren A (1993) Availability, uptake, and turnover of glycerol in hypersaline environments. FEMS Microbiol Ecol 12:15–23.CrossRefGoogle Scholar
  83. Oren A (1994) Characterization of the halophilic archaeal community in saltern crystallizer ponds by means of polar lipid analysis. Int J Salt Lake Res 3:15–29.CrossRefGoogle Scholar
  84. Oren A (1995a) Uptake and turnover of acetate in hypersaline environments. FEMS Microbiol Ecol 18;75–84.CrossRefGoogle Scholar
  85. Oren A (1995b) The role of glycerol in the nutrition of halophilic archaeal communities: a study of respiratory electron transport. FEMS Microbiol Ecol 16:281–290.CrossRefGoogle Scholar
  86. Oren A (1999) Bioenergetic aspects of halophilism. Microbiol Mol Biol Rev 63:334–348.PubMedGoogle Scholar
  87. Oren A (2001) The bioenergetic basis for the decrease in metabolic diversity in increasing salt concentrations: implications for the functioning of salt lake ecosystems. Hydrobiologia 466:61–72.CrossRefGoogle Scholar
  88. Oren A (2002a) Halophilic microorganisms and their environments. Kluwer Scientific Publishers, Dordrecht.CrossRefGoogle Scholar
  89. Oren A (2002b) Molecular ecology of extremely halophilic Archaea and Bacteria. FEMS Microbiol Ecol 39:1–7.CrossRefGoogle Scholar
  90. Oren A (2006) Life at high salt concentrations. In: Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E (eds), The prokaryotes. A handbook on the biology of bacteria: Ecophysiology and biochemistry, vol. 2. Springer, New York, NY.Google Scholar
  91. Oren A (2009) Microbial diversity and microbial abundance in salt-saturated brines: why are the waters of hypersaline lakes red? In: Oren A, Naftz DL, Palacios P, Wurtsbaugh WA (eds), Saline lakes around the world: Unique systems with unique values, The S.J. and Jessie E. Quinney Natural Resources Research Library, College of Natural Resources, Utah State University, Salt Lake City, UT.Google Scholar
  92. Oren A (2011) Thermodynamic limits to microbial life at high salt concentrations. Environ Microbiol 13:1908–1923.PubMedCrossRefGoogle Scholar
  93. Oren A, Ben-Yosef N (1997) Development and spatial distribution of an algal bloom in the Dead Sea: A remote sensing study. Aquat Microb Ecol 13:219–223.CrossRefGoogle Scholar
  94. Oren A, Dubinsky Z (1994) On the red coloration of saltern crystallizer ponds. II. Additional evidence for the contribution of halobacterial pigments. Int J Salt Lake Res 3:9–13.CrossRefGoogle Scholar
  95. Oren A, Gurevich P (1993) Characterization of the dominant halophilic archaea in a bacterial bloom in the Dead Sea. FEMS Microbiol Ecol 12:249–256.CrossRefGoogle Scholar
  96. Oren A, Gurevich P (1994) Production of D-lactate, acetate, and pyruvate from glycerol in communities of halophilic archaea in the Dead Sea and in saltern crystallizer ponds. FEMS Microbiol Ecol 14:147–156.Google Scholar
  97. Oren A, Gurevich P (1995) Dynamics of a bloom of halophilic archaea in the Dead Sea. Hydrobiologia 315:149–158.CrossRefGoogle Scholar
  98. Oren A, Rodríguez-Valera F (2001) The contribution of Salinibacter species to the red coloration of saltern crystallizer ponds. FEMS Microbiol Ecol 36:123–130.PubMedGoogle Scholar
  99. Oren A, Shilo M (1981) Bacteriorhodopsin in a bloom of halobacteria in the Dead Sea. Arch Microbiol 130:185–187.CrossRefGoogle Scholar
  100. Oren A, Shilo M (1982) Population dynamics of Dunaliella parva in the Dead Sea. Limnol Oceanogr 27;201–211.CrossRefGoogle Scholar
  101. Oren A, Stambler N, Dubinsky Z (1992) On the red coloration of saltern crystallizer ponds. Int J Salt Lake Res 1:77–89.CrossRefGoogle Scholar
  102. Oren A, Fischel U, Aizenshtat Z, Krein EB, Reed RH (1994) Osmotic adaptation of microbial communities in hypersaline microbial mats. In: Stal LJ, Caumette P (eds), Microbial mats. Structure, development and environmental significance. Springer-Verlag, Berlin.Google Scholar
  103. Oren A, Gurevich P, Anati DA, Barkan E, Luz B (1995a) A bloom of Dunaliella parva in the Dead Sea in 1992: biological and biogeochemical aspects. Hydrobiologia 297:173–185.CrossRefGoogle Scholar
  104. Oren A, Kühl M, Karsten U (1995b) An endoevaporitic microbial mat within a gypsum crust: zonation of phototrophs, photopigments, and light penetration. Mar Ecol Prog Ser 128:151–159.CrossRefGoogle Scholar
  105. Oren A, Duker S, Ritter S (1996) The polar lipid composition of Walsby’s square bacterium. FEMS Microbiol Lett 138:135–140.CrossRefGoogle Scholar
  106. Oren A, Bratbak G, Heldal M (1997) Occurrence of virus-like particles in the Dead Sea. Extremophiles 1:143–149.PubMedCrossRefGoogle Scholar
  107. Oren A, Ionescu D, Lipski A, Altendorf K (2005) Fatty acid analysis of a layered community of cyanobacteria developing in a hypersaline gypsum crust. Algol Stud 117:339–347.CrossRefGoogle Scholar
  108. Oren A, Sørensen KB, Canfield DE, Teske AP, Ionescu D, Lipski A, Altendorf K (2009) Microbial communities and processes within a hypersaline gypsum crust in a saltern evaporation pond (Eilat, Israel). Hydrobiologia 626:15–26.CrossRefGoogle Scholar
  109. Øvreås L, Daae FL, Torsvik T, Rodríguez-Valera F (2003) Characterization of microbial diversity in hypersaline environments by melting profiles and reassociation kinetics in combination with terminal restriction fragment length polymorphism (T-RFLP). Microb Ecol 46:291–301.PubMedCrossRefGoogle Scholar
  110. Pagaling E, Wang H, Venables M, Wallace A, Grant WD, Cowan DA, Jones BE, Ma Y, Ventosa A, Heaphy S (2009) Microbial biogeography of six salt lakes in Inner Mongolia, China, and a salt lake in Argentina. Appl Environ Microbiol 75:5750–5760.PubMedCrossRefGoogle Scholar
  111. Parnell JJ, Rompato G, Latta LC, IV, Pfender ME, Van Nostrand JD, He Z, Zhou J, Andersen G, Champine P, Ganesan B, Weimer BC (2010) Functional biogeography as evidence of gene transfer in hypersaline microbial communities. PLoS One 5:e12919.CrossRefGoogle Scholar
  112. Pašić L, Galán Bartual S, Poklar Ulrih N, Grabnar M, Herzog Velikonja B (2005) Diversity of halophilic archaea in the crystallizers of an Adriatic solar saltern. FEMS Microbiol Ecol 54:491–498.PubMedCrossRefGoogle Scholar
  113. Pašić L, Poklar Ulrih N, Črnigoj M, Grabnar M, Herzog Velikonja B (2007) Haloarchaeal communities in the crystallizers of two Adriatic solar salterns. Can J Microbiol 53:8–18.PubMedCrossRefGoogle Scholar
  114. Pedrós-Alió C, Calderón-Paz JI, MacLean MH, Medina G, Marassé C, Gasol JM, Guixa-Boixereu N (2000a) The microbial food web along salinity gradients. FEMS Microbiol Ecol 32:143–155.CrossRefGoogle Scholar
  115. Pedrós-Alió C, Calderón-Paz JI, Gasol JM (2000b) Comparative analysis shows that bacterivory, not viral lysis, controls the abundance of heterotrophic prokaryotic plankton. FEMS Microbiol Ecol 32:157–165.CrossRefGoogle Scholar
  116. Porter D, Roychoudhury AN, Cowan D (2007) Dissimilatory sulfate reduction in hypersaline coastal pans: Activity across a salinity gradient. Geochim Cosmochim Acta 71:5102–5116.CrossRefGoogle Scholar
  117. Prášil O, Bina D, Medová H, Řeháková K, Zapomělová E, Veselá J, Oren A (2009) Emission spectroscopy and kinetic fluorimetry studies of the phototrophic microbial communities along the salinity gradient in the solar saltern evaporation ponds of Eilat, Israel. Aquat Microb Ecol 56:285–296.CrossRefGoogle Scholar
  118. Rees HC, Grant WD, Jones BE, Heaphy S (2004) Diversity of Kenyan soda lake alkaliphiles assessed by molecular methods. Extremophiles 8:63–71.PubMedCrossRefGoogle Scholar
  119. Roberts MF (2006) Characterization of organic compatible solutes of halotolerant and halophilic microorganisms. In: Rainey FA, Oren A (eds), Extremophiles—Methods in microbiology, vol. 35. Elsevier/Academic Press, AmsterdamGoogle Scholar
  120. Robertson CE, Spear JR, Harris JK, Pace NR (2009) Diversity and stratification of archaea in a hypersaline microbial mat. Appl Environ Microbiol 75:1801–1810.PubMedCrossRefGoogle Scholar
  121. Rodriguez-Brito B, Li L, Wegley L, Furlam M, Angly F, Breitbart M, Buchanan J, Desnues C, Dinsdale E, Edwards R, Felts B, Haynes M, Liu H, Lipson D, Mahaffy J, Martin-Cuadrado AB, Mira A, Nulton J, Pašić L, Rayhawk S, Rodriguez-Mueller J, Rodriguez-Valera F, Salamon P, Srinagesh S, Thingstad TF, Tran T, Thurber RV, Willner D, Youle M, Rohwer F (2010) Viral and microbial community dynamics in four aquatic environments. ISME J 4:739–751.PubMedCrossRefGoogle Scholar
  122. Rosselló-Mora R, Lee N, Antón J, Wagner M (2003) Substrate uptake in extremely halophilic microbial communities revealed by microautoradiography and fluorescence in situ hybridization. Extremophiles 7:409–413.PubMedCrossRefGoogle Scholar
  123. Rosselló-Mora R, Lucio M, Peña A, Brito-Echeverría J, López-López A, Valens-Vadell M, Frommberger M, Antón J, Schmitt-Kopplin P (2008) Metabolic evidence for biogeographic isolation of the extremophilic bacterium Salinibacter ruber. ISME J 2:242–253.PubMedCrossRefGoogle Scholar
  124. Sabet S (2012) Halophilic viruses. Springer, DordrechtGoogle Scholar
  125. Sandaa R-A, Skjodal EF, Bratbak G (2003) Virioplankton community structure along a salinity gradient in a solar saltern. Extremophiles 7:347–351.PubMedCrossRefGoogle Scholar
  126. Santos F, Meyerdierks A, Peña A, Rosselló-Mora R, Amann R, Antón J (2007) Metagenomic approach to the study of halophages: the environmental halophage 1. Environ Microbiol 9:1711–1723.PubMedCrossRefGoogle Scholar
  127. Santos F, Moreno-Paz M, Meseguer I, López C, Rosselló-Mora R, Parro V, Antón J (2011) Metatranscriptomic analysis of extremely halophilic viral communities. ISME J 5:1621–1633.PubMedCrossRefGoogle Scholar
  128. Santos F, Yarza P, Parro V, Meseguer I, Rosselló-Mora R, Antón J (2012) Culture-independent approaches for studying viruses from hypersaline environments. Appl Environ Microbiol 78:1635–1643.PubMedCrossRefGoogle Scholar
  129. Scholten JCM, Joye SB, Hollibaugh JT, Murrell JC (2005) Molecular analysis of the sulfate reducing and archaeal community in a meromictic soda lake (Mono Lake, California) by targeting 16S rRNA, mcrA, apsA, and dsrAB genes. Microb Ecol 50:29–39.PubMedCrossRefGoogle Scholar
  130. Shand RF (2006) Detection, quantification and purification of halocins: peptide antibiotics from haloarchaeal extremophiles. In: Rainey FA, Oren A (eds), Extremophiles—Methods in microbiology, vol. 35. Elsevier/Academic Press, Amsterdam.Google Scholar
  131. Sher J, Elevi R, Mana L, Oren A (2004) Glycerol metabolism in the extremely halophilic bacterium Salinibacter ruber. FEMS Microbiol Lett 232:211–215.PubMedCrossRefGoogle Scholar
  132. Sime-Ngando T, Lucas S, Robin A, Pause Tucker K, Colombet J, Forterre P, Breitbart M, Prangishvili D (2011) Diversity of virus-host systems in hypersaline Lake Retba, Senegal. Environ Microbiol 13:1956–1972.PubMedCrossRefGoogle Scholar
  133. Sørensen KB, Canfield DE, Oren A (2004) Salt responses of benthic microbial communities in a solar saltern (Eilat, Israel). Appl Environ Microbiol 70:1608–1616.PubMedCrossRefGoogle Scholar
  134. Sørensen KB, Canfield DE, Teske AP, Oren A (2005) Community composition of a hypersaline endoevaporitic microbial mat. Appl Environ Microbiol 71:7352–7365.PubMedCrossRefGoogle Scholar
  135. Stan-Lotter H, Leuko S, Legat A, Fendrihan S (2006) The assessment of the viability of halophilic microorganisms in natural communities. In: Rainey FA, Oren A (eds), Extremophiles—Methods in microbiology, vol. 35. Elsevier/Academic Press, Amsterdam.Google Scholar
  136. Stephens DW, Gillespie DM (1976) Phytoplankton production in the Great Salt Lake, Utah, and a laboratory study of algal response to enrichment. Limnol Oceanogr 21:74–87.CrossRefGoogle Scholar
  137. Stoeckenius W, Bivin D, McGinnis K (1985) Photoactive pigments in halobacteria from the Gavish sabkha. In: Friedman GM, Krumbein WE (eds), Hypersaline ecosystems. The Gavish sabkha. Springer-Verlag, Berlin.Google Scholar
  138. Stock A, Breiner H-W, Pachiadaki M, Edgcomb V, Filker S, La Cono V, Yakimov MM, Stoeck T (2012) Microbial eukaryote life in the new hypersaline deep-sea basin Thetis. Extremophiles 16:21–34.PubMedCrossRefGoogle Scholar
  139. Vaisman A, Oren A (2009) Salisaeta longa gen. nov., sp. nov., a red halophilic bacterium isolated from an experimental mesocosm at Sedom, Israel. Int J Syst Evol Microbiol 59:2571–2574.PubMedCrossRefGoogle Scholar
  140. Valenzuela-Encinas C, Neria-González I, Alcántara-Hernández RJ, Enríquez-Aragón JA, Estrada-Alvarado I, Hernández-Rodríguez C, Dendooven L, Marsch R (2008) Phylogenetic analysis of the archaeal community in an alkaline-saline soil of the former lake Texcoco (Mexico). Extremophiles 12:247–254.PubMedCrossRefGoogle Scholar
  141. Van Der Wielen PWJJ, Heijs SK (2007) Sulfate-reducing prokaryotic communities in two deep hypersaline anoxic basins in the Eastern Mediterranean deep sea. Environ Microbiol 9:1335–1340.PubMedCrossRefGoogle Scholar
  142. Wais AC, Daniels LL (1985) Populations of bacteriophage infecting Halobacterium in a transient brine pool. FEMS Microbiol Ecol 31:323–326.CrossRefGoogle Scholar
  143. Wang C-Y, Ng C-C, Chen T-W, Wu S-J, Shyu Y-T (2007) Microbial diversity analysis of former salterns in southern Taiwan by 16S rRNA-based methods. J Basic Microbiol 7:525–533.CrossRefGoogle Scholar
  144. Warkentin M, Schumann R, Oren A (2009) Community respiration studies in saltern crystallizer ponds. Aquat Microb Ecol 56:255–261.CrossRefGoogle Scholar
  145. Welsh DT (2000) Ecological significance of compatible solute accumulation by micro-organisms: from single cells to global climate. FEMS Microbiol Rev 24:263–290.PubMedCrossRefGoogle Scholar
  146. Wu QL, Zwart G, Schauer M, Kamst-van Agterveld MP, Hahn MW (2006) Bacterioplankton community composition along a salinity gradient of sixteen high-mountain lakes located on the Tibetan plateau, China. Appl Environ Microbiol 72:5478–5485.PubMedCrossRefGoogle Scholar
  147. Youssef NH, Ashlock-Savage KN, Elshahed M (2012) Phylogenetic diversities and community structure of members of the extremely halophilic Archaea (order Halobacteriales) in multiple saline sediment habitats. Appl Environ Microbiol 78:1332–1344.PubMedCrossRefGoogle Scholar
  148. Zalar P, Kocuvan MA, Plemenitaš A, Gunde-Cimerman N (2005) Halophilic black yeasts colonize wood immersed in hypersaline water. Bot Mar 48:323–326.CrossRefGoogle Scholar

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© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  1. 1.Department of Plant and Environmental Sciences, The Institute of Life SciencesThe Hebrew University of JerusalemJerusalemIsrael

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