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Plant Regeneration and Genetic Transformation in Jatropha

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Biotechnology of Neglected and Underutilized Crops

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Abstract

Jatropha curcas, a non-edible oil bearing species with multiple uses, and considerable economic potential is emerging as a potential biofuel plant. The limited knowledge of this species, low and inconsistent yields, the narrow genetic variability, and vulnerability to insects and diseases are major constraints in successful cultivation of Jatropha as a biofuel crop. Hence, genetic improvement of Jatropha is essential by conventional and modern biotechnological tools to use as a viable alternative source of bio-diesel. Realising its potential as a bio-energy crop, in vitro regeneration methods have been established to meet the demand of large scale supply of superior clones, and also as a prelude for genetic improvement of the species through transgenic approaches. In this chapter, an overview of in vitro tissue culture and genetic transformation of Jatropha is discussed.

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Abbreviations

NAA:

α-napthaleneacetic acid

BA:

Benzyl adenine

MS:

Murashige and Skoog

Kn:

6-furfurylaminopurine

IBA:

Indole-3-butyric acid

TDZ:

Thidiazuron

AFLP:

Amplified fragment length polymorphism

ISSRs:

Inter simple sequence repeats

MSAP:

Methylation sensitive amplified polymorphism

References

  • Abigor RD, Uadia PO, Foglia TA, Haas MJ, Scott K, Savary BJ (2002) Partial purification and properties of lipase from germinating seeds of Jatropha curcas L. J Amer Oil Chem Soc 79:1123–1126

    Article  CAS  Google Scholar 

  • Adebowale KO, Adedire CO (2006) Chemical composition and insecticidal properties of the underutilized Jatropha curcas seed oil. Afr J Biotechnol 10:901–906

    Google Scholar 

  • Agarwal M, Shrivastava N, Padh H (2008) Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Rep 27:617–631

    Article  PubMed  CAS  Google Scholar 

  • Ahuja MR (1987) Gene transfer in forest trees. In: Hanover JW, Keathley DE (eds) Genetic manipulation of woody plants. Plenum Press, New York, pp 25–41

    Google Scholar 

  • Alain H, Ali K, Danielle L (2004) Lipases and their industrial applications: an overview. Appl Biochem Biotechnol 118:155–170

    Article  Google Scholar 

  • Altei WF, Picchi DG, Barbosa SC et al (2008) NMR studies, solid phase synthesis and MD/SA simulation as a tool for structural elucidation of new bioactive peptides from the latex of Jatropha curcas L. Planta Med 74:1–338 (SL65)

    Google Scholar 

  • Auvin C, Baraguey C, Blond A et al (1997) Curcacycline B, a cyclic nonapeptide from Jatropha curcas enhancing rotamase activity of cyclophilin. Tetrahedron Lett 38:2845–2848

    Article  CAS  Google Scholar 

  • Baraguey C, Blond A, Cavelier F et al (2001) Isolation, structure and synthesis of mahafacyclin B, a cyclic heptapeptide from the latex of Jatropha mahafalensis. J Chem Soc Perkin Trans 1:2098–2103

    Article  CAS  Google Scholar 

  • Becker K, Makkar HPS (1998) Toxic effects of phorbol esters in carp (Cyprinus carpio L). Vet Hum Toxicol 40:82–86

    Google Scholar 

  • Berchmans HJ, Hirata S (2008) Biodiesel production from crude Jatropha curcas L. seed oil with a high content of free fatty acids. Bioresour Technol 99:1716–1721

    Article  PubMed  CAS  Google Scholar 

  • Carels N (2009) Jatropha curcas: a review. Adv Bot Res 50:39–86

    Article  CAS  Google Scholar 

  • Carvalho CR, Clarindo WR, Praca MM et al (2008) Genome size, base composition and karyotype of Jatropha curcas L., an important biofuel plant. Plant Sci 174:613–617

    Article  CAS  Google Scholar 

  • Chitra P, Venkatachalam P, Sampathrajan A (2005) Optimization of experimental conditions for biodiesel production from alkali-catalysed transesterification of Jatropha curcas oil. Energy Sustain Dev 9:13–18

    Article  Google Scholar 

  • da Camara Machado A, Frick NS, Kremen R et al (1997) Biotechnological approaches to the improvement of Jatropha curcas. In: Proceedings of the International Symposium on Jatropha pp. 15 Managua, Nicaragua

    Google Scholar 

  • Datta MM, Mukherjee P, Ghosh B et al (2007) In vitro clonal propagation of biodiesel plant (Jatropha curcas L). Curr Sci 93:1438–1442

    CAS  Google Scholar 

  • Dehgan B (1984) Phylogenetic significance of interspecific hybridization in Jatropha (Euphorbiaceae). Syst Bot 9:467–478

    Article  Google Scholar 

  • Deore AC, Johnson TS (2008) High-frequency plant regeneration from leaf-disc cultures of Jatropha curcas L.: an important biodiesel plant. Plant Biotechnol Rep 2:7–11

    Google Scholar 

  • Fairless D (2007) Biofuel: the little shrub that could-maybe. Nature 449:652–655

    Article  PubMed  Google Scholar 

  • Francis G, Edinger R, Becker K (2005) A concept for simultaneous waste land reclamation, fuel production, and socio-economic development in degraded areas in India: need, potential and perspectives of Jatropha plantations. Nat Resourc Forum 29:12–24

    Article  Google Scholar 

  • Ghosh A, Chaudhary DR, Reddy MP et al (2007) Prospects for Jatropha methyl ester (biodiesel) in India. Int J Environ Stud 64:659–674

    Article  CAS  Google Scholar 

  • Ginwal US, Rawat PS, Srivastava RL (2004) Seed source variation in growth performance and oil yield of Jatropha curcas L in Central India. Silvae Genet 53:186–192

    Google Scholar 

  • Gressel J (2008) Transgenics are imperative for biofuel crops. Plant Sci 174:246–263

    Article  CAS  Google Scholar 

  • Gubitz GM, Mittelbach M, Trabi M (1999) Exploitation of the tropical oil seed plant Jatropha curcas L. Bioresour Technol 67:73–82

    Article  CAS  Google Scholar 

  • Gupta RC (1985) Pharmacognostic studies on ‘Dravanti’. Part I. Jatropha curcas Linn. Indian Acad Sci 1:65–81

    Google Scholar 

  • Hamilton C, Frary A, Lewis C et al (1997) Stable transfer of intact high molecular weight DNA into plant chromosomes. Proc Natl Acad Sci USA 93:9975–9979

    Article  Google Scholar 

  • He Y, Pasapula V, Li X et al (2009a) Agrobacterium tumefaciens-mediated transformation of Jatropha curcas: factors affecting transient transformation efficiency and morphology analysis of transgenic calli. Silvae Genet 58:123–128

    Google Scholar 

  • He Y, Guo X, Lu R et al (2009b) Changes in morphology and biochemical indices in browning callus derived from Jatropha curcas hypocotyls. Plant Cell Tiss Org Cult 98:11–17

    Article  CAS  Google Scholar 

  • Heller J (1996) Physic nut Jatropha curcas L In: Promoting the Conservation and use of Underutilized and Neglected Crops. 1. Institute of Plant Genetics and Crop Plant Research. Gatersleben/International Plant Genetic Resources Institute, Rome

    Google Scholar 

  • Hernandez JBP, Remy S, Sauco VG et al (1999) Chemotactic movement and attachment of Agrobacterium tumefaciens to banana cells and tissues. J Plant Physiol 155:245–250

    Article  Google Scholar 

  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss Org Cult 33:105–119

    Article  CAS  Google Scholar 

  • Ingelbrecht I, Breyne P, Vancomperonolle A et al (1991) Transcriptional interferences in transgenic plants. Gene 109:239–242

    Article  PubMed  CAS  Google Scholar 

  • Jha TB, Mukherjee P, Datta MM (2007) Somatic embryogenesis in Jatropha curcas Linn., an important biofuel plant. Plant Biotechnol Rep 1:135–140

    Article  Google Scholar 

  • Jin-xia Z, Qin W, Li-jun Z et al (2005) The extraction of β-1,3-glucanase and analysis of partial characteristics in Jatropha curcas. Southwest China J Agric Sci 18:328–333

    Google Scholar 

  • Johri BM, Bhojwani SS (1965) Growth response of mature endosperm in cultures. Nature 298:1345–1347

    Article  Google Scholar 

  • Jones N, Miller JH (1991) Jatropha curcas a multipurpose species for problematic sites. Land Resour Ser 1:1–12

    Google Scholar 

  • Jongschaap REE, Corré WJ, Bindraban OS et al (2007) Claims and facts on Jatropha curcas L.: Wageningen, The Netherlands: Plant Research International. http;//factfuels.org/media_en/Climes_and_Facts_on_Jatropha_WUR? Session = isgsklbna58j7grrfst888n5r7

    Google Scholar 

  • Josephina GN, van Staden J (1990) The relationship between genotype, tissue age and endogenous cytokinin levels on adventitious bud formation on leaves of Lachenalia. Plant Cell Tiss Org Cult 22:223–228

    Article  Google Scholar 

  • Joshi M, Mishra A, Jha B (2011) Efficient genetic transformation of Jatropha curcas L. by microprojectile bombardment using embryo axes. Ind Crops Prod 33:67–77

    Article  CAS  Google Scholar 

  • Kaewpoo M, Te-chato S (2010) Study on ploidy level of micropropagated Jatropha curcas L. via flow cytometry. J Agri Tech 6:391–400

    Google Scholar 

  • Kalimuthu K, Paulsamy S, Senthil Kumar R et al (2007) In vitro propagation of bio-diesel plant of Jatropha curcas L. Plant Tiss Cult Biotech 17:137–147

    Google Scholar 

  • Karp A (1994) Origins, causes and uses of variation in plant tissue cultures. In: Vasil IK, Thorpe TA (eds) Plant Cell and Tissue Culture. Kluwer Acad Publ Dordrecht, The Netherlands, pp 139–151

    Google Scholar 

  • Khemkladngoen N, Caragena J, Shibagaki N et al (2011) Adventitious shoot regeneration from juvenile cotyledons of a biodiesel producing plant, Jatropha curcas L. J Biosci Bioeng 111:67–70

    Article  PubMed  CAS  Google Scholar 

  • Khurana-Kaul V, Kachhwaha S, Kothari SL (2010) Direct shoot regeneration from leaf explants of Jatropha curcas in response to thidiazuron and high copper contents in the medium. Biol Plant 54:369–372

    Article  CAS  Google Scholar 

  • Kochhar S, Kochhar VK, Singh SP et al (2005) Differential rooting and sprouting behaviour of two Jatropha species and associated physiological and biochemical changes. Curr Sci 89:936–939

    Google Scholar 

  • Konez C, Martini N, Mayerhofer R et al (1989) High frequency T-DNA mediated tagging in plants. Proc Nat Acad Sci USA 86:8467–8471

    Article  Google Scholar 

  • Kothari SL, Varshney A (1998) Morphogenesis in long term maintained immature embryo derived callus of wheat (Triticum aestivum L.): histological evidence for both somatic embryogenesis and organogenesis. Plant Biochem Biotech 7:93–98

    Article  Google Scholar 

  • Krishna G, Sairam Reddy P, Bhattacharya PS et al (2010) Shoot organogenesis and plantlet regeneration from in vitro raised leaf segments of Jatropha curcas L. Trends Biosci 3:45–48

    Google Scholar 

  • Kumar N (2008) Studies on regeneration and genetic transformation in J. curcas. PhD thesis, Bhavngar University, Bhavngar, India

    Google Scholar 

  • Kumar N, Reddy MP (2010) Plant regeneration through the direct induction of shoot buds from petiole explants of Jatropha curcas: a biofuel plant. Ann Appl Biol 156:367–375

    Article  CAS  Google Scholar 

  • Kumar A, Sharma S (2008) An evaluation of multipurpose oil seed crop for industrial uses: a review. Ind Crops Prod 28:1–10

    Article  CAS  Google Scholar 

  • Kumar S, Dhingra A, Daniell H (2004) Plastid-expressed betaine aldehyde dehydrogenase gene in carrot cultured cells, roots, and leaves confers enhanced salt tolerance. Plant Physiol 136:2843–2854

    Article  PubMed  CAS  Google Scholar 

  • Kumar N, Vijayanand KG, Reddy MP (2010a) Shoot regeneration from cotyledonary leaf explants of Jatropha curcas: a biodesel plant. Acta Physiol Plant 32:917–924

    Article  Google Scholar 

  • Kumar N, Vijayanand KG, Reddy MP (2010b) In vitro plant regeneration of non-toxic Jatropha curcas L: direct shoot organogenesis from cotyledonary petiole explants. J Crop Sci Biotech 13:189–194

    Article  Google Scholar 

  • Kumar N, Vijayanand KG, Pamidimarri DVNS et al (2010c) Stable genetic transformation of Jatropha curcas via Agrobacterium tumefaciens-mediated gene transfer using leaf explants. Ind Crops Prod 32:41–47

    Article  CAS  Google Scholar 

  • Kumar N, Vijayanand KG, Reddy MP (2011) In vitro regeneration from petiole explants of non-toxic Jatropha curcas. Ind Crops Prod 33:146–151

    Article  CAS  Google Scholar 

  • Leela T, Naresh B, Srikanth Reddy M et al (2011) Morphological, physico-chemical and micropropagation studies in Jatropha curcas L. and RAPD analysis of the regenerants. Appl Energy 88:2071–2079

    Article  CAS  Google Scholar 

  • Li MR, Li HQ, Wu GJ (2006) Study on factors influencing Agrobacterium-mediated transformation of Jatropha curcas. J Mol Cell Biol 39:83–87

    CAS  Google Scholar 

  • Li M, Li H, Jiang H et al (2008) Establishment of an Agrobacterium-mediated cotyledon disc transformation method for Jatropha curcas. Plant Cell Tiss Organ Cult 92:173–181

    Article  CAS  Google Scholar 

  • Li C, Yu M, Chen F et al (2010) In vitro maturation and germination of Jatropha curcas microspores. Intl J Agric Biol 12:541–546

    CAS  Google Scholar 

  • Lin J, Tang L, Chen F (2002a) Tissue culture and plantlet regeneration of Jatropha curcas. Plant Physiol Commun 38:252

    Google Scholar 

  • Lin J, Yan F, Tang L et al (2002b) Isolation, purification and functional investigation on the N-glycosidase activity of curcin from the seeds of Jatropha curcas. High Technol Lett 11:36–40

    Google Scholar 

  • Liu HF, Kirchoff BK, Wu GJ et al (2007) Microsporogenesis and male gametogenesis in Jatropha curcas L. (Euphorbiaceae). J Torrey Bot cL 134:335–343

    Article  Google Scholar 

  • Lu WD, Wei Q, Tang L et al (2003) Induction of callus from Jatropha curcas and rapid propagation. Chin J Appl Environ Biol 9:127–130

    CAS  Google Scholar 

  • Makkar HPS, Becker K, Schmook B (1998) Edible provenances of Jatropha curcas from Quintana Roo state of Mexico and effect of roasting on antinutrient and toxic factors in seeds. Plant Foods Human Nutr 52:1–36

    Article  Google Scholar 

  • Martins M, Sarmento D, Oliveira MM (2004) Genetic stability of micropropagated almond plantlets as assessed by RAPD and ISSR markers. Plant Cell Rep 23:492–496

    Article  PubMed  CAS  Google Scholar 

  • Mazumdar P, Basu A, Paul A et al (2010) Age and orientation of the cotyledonary leaf explants determine the efficiency of de novo plant regeneration and Agrobacterium tumefaciens mediated transformation in Jatropha curcas L. South Afr J Bot 76:337–344

    Article  Google Scholar 

  • Minocha SC (1987) Plant growth regulators and morphogenesis in cell and tissue cultures of forest trees. In: Bonga JM, Durzan DJ (eds) Cell and Tissue Culture in Forestry, Vol I, pp 50–66. Martinus Nijhoff Publisher, Dordrecht

    Google Scholar 

  • Misra P, Gupta N, Toppo DD et al (2010a) Establishment of long-term proliferating shoot cultures of elite Jatropha curcas L. by controlling endophytic bacterial contamination. Plant Cell Tiss Org Cult 100:189–197

    Article  Google Scholar 

  • Misra P, Toppo DD, Gupta N et al (2010b) Effect of antioxidants and associate changes in antioxidant enzymes in controlling browning and necrosis of proliferating shoots of elite Jatropha curcas L. Biomass Bioenergy 34:1861–1869

    Article  CAS  Google Scholar 

  • Misra P, Toppo DD, Mishra MK et al (2011) Agrobacterium tumefaciens-mediated transformation protocol of Jatropha curcas L. using leaf and hypocotyl segments. Plant Biochem Biotechnol. doi:101007/s13562-011-0072-3

    Google Scholar 

  • Naruemon K, Cartagena JA, Kiichi F (2011) Physical wounding assisted Agrobacterium mediated transformation of juvenile cotyledons of a biodiesel producing plant, Jatropha curcas L. Plant Biotechnol Rep 5:235–243

    Article  Google Scholar 

  • Nath LK, Dutta SK (1992) Wound healing response of the proteolytic enzyme curcain. Indian J Pharmacol 24:114–115

    Google Scholar 

  • Openshaw K (2000) A review of Jatropha curcas: an oil plant unfulfilled promise. Biomass Bioenergy 19:1–15

    Article  Google Scholar 

  • Ostry ME, Hackett W, Michler C et al (1994) Influence of regeneration method and tissue source on the frequency of somatic variation in Populus to infection by Septoria musiva. Plant Sci 97:5222–5226

    Article  Google Scholar 

  • Palombi MA, Damiano C (2002) Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit (Actinidia deliciosa A. Chev). Plant Cell Rep 20:1061–1066

    Article  CAS  Google Scholar 

  • Pan J, Fu Q, Xu ZF (2010) Agrobacterium tumefaciens-mediated transformation of biofuel plant Jatropha curcas using kanamycin selection. Afr J Biotech 9:6477–6481

    CAS  Google Scholar 

  • Prabakaran AJ, Sujatha M (1999) Jatropha tanjorensis Ellis and Saroja, a natural interspecific hybrid occurring in Tamil Nadu, India. Genet Resour Crop Evol 46:213–218

    Article  Google Scholar 

  • Preece JE, Imel MR (1991) Plant regeneration from leaf explants of Rhododendron ‘P.J.M. Hybrids’. Sci Hortic 48:159–170

    Article  CAS  Google Scholar 

  • Purkayastha J, Sugla T, Paul A et al (2010) Efficient in vitro plant regeneration from shoot apices and gene transfer by particle bombardment in Jatropha curcas. Biol Plant 54:13–20

    Article  CAS  Google Scholar 

  • Rajore S, Batra A (2005) Efficient plant regeneration via shoot tip explant in Jatropha curcas. J Plant Biochem Biotechnol 14:73–75

    Article  Google Scholar 

  • Rajore S, Batra A (2007) An alternative source for regenerable organogenic callus induction in Jatropha curcas. Indian J Biotech 6:545–548

    Google Scholar 

  • Rajore S, Sardana J, Batra A (2002) In vitro cloning of Jatropha curcas L. J Plant Biol 29:195–198

    Google Scholar 

  • Rani V, Raina SN (2000) Genetic fidelity of organized meristem derived micropropagated plants: a critical reappraisal. In Vitro Cell Dev Biol Plant 36:319–330

    Article  CAS  Google Scholar 

  • Reddy MP and Pamidimarri DVNS (2010) Biology and biotechnological advances in Jatropha curcas—a biodiesel plant. Ramawat KG (ed) Desert Plants. Springer, Berlin Heidelberg

    Google Scholar 

  • Reddy MP, Kumar N, Vijay Anand KG et al (2008) Method for micropropagation of Jatropha curcas plants from leaf explants. Patent filed US and PCT, Application No. 2537de2008

    Google Scholar 

  • Rupert EA, Dehgan B, Webster GL (1970) Experimental studies of relationships in the genus Jatropha L. J. curcas × J. integerrima. Bull Torrey Bot Cl 97:321–325

    Article  Google Scholar 

  • Saker MM, Adawy SS, Mohamed AA et al (2006) Monitoring of cultivar identity in tissue culture-derived date palms using RAPD and AFLP analysis. Biol Plant 50:198–204

    Article  CAS  Google Scholar 

  • Sardana J, Batra A, Sharma R (1998) In vitro plantlet formation and micropropagation of Jatropha curcas L. Adv Plant Sci 11:167–169

    Google Scholar 

  • Sardana J, Batra A, Ali DJ (2000) An expeditious method for regeneration of somatic embryos in Jatropha curcas L. Phytomorphology 50:239–242

    Google Scholar 

  • Sato S, Hirakawa H, Isobe S et al (2011) Sequence analysis of the genome of an oil bearing tree, Jatropha curcas L. DNA Res 18:65–76

    Article  PubMed  CAS  Google Scholar 

  • Sharma A, Kansal N, Shekhawat GS (2006) In vitro culture and plant regeneration of economically potent plant species Jatropha curcas. Biochem Cell Arch 6:323–327

    CAS  Google Scholar 

  • Sharma S, Kumar N, Reddy MP (2011a) Regeneration in Jatropha curcas: Factors affecting the efficiency of in vitro regeneration. Ind Crops Prod 34:943–951

    Article  CAS  Google Scholar 

  • Sharma S, Sudheer PDVN, Vijay Anand KG et al (2011b) Assessment of genetic stability in micropropagules of Jatropha curcas genotypes by RAPD and AFLP analysis. Ind Crops Prod 34:1003–1009

    Article  CAS  Google Scholar 

  • Shen X, Chen J, Kane ME et al (2007) Assessment of somaclonal variation in Dieffenbachia plants regenerated through indirect shoot organogenesis. Plant Cell Tiss Org Cult 91:21–27

    Article  Google Scholar 

  • Shrivastava S, Banerjee M (2008) In vitro clonal propagation of physic nut (Jatropha curcas L): Influence of additives. Intl J Integrative Biol 3:73–79

    CAS  Google Scholar 

  • Singh A, Reddy MP, Chikara J et al (2010) A simple regeneration protocol from stem explants of Jatropha curcas—a biodiesel plant. Ind Crops Prod 31:209–213

    Article  CAS  Google Scholar 

  • Smykal P, Valledor L, Rodriguez R et al (2007) Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of pea (Pisum sativum L.). Plant Cell Rep 26:1985–1998

    Article  PubMed  CAS  Google Scholar 

  • Soomro R, Memon RA (2007) Establishment of callus and suspension culture in Jatropha curcas. Pak J Bot 39:2431–2441

    Google Scholar 

  • Srivastava PS (1971) In vitro induction of triploid roots and shoots from mature endosperm of Jatropha panduraefolia. Z Pflanzenphysiol 66:93–96

    CAS  Google Scholar 

  • Srivastava PS, Johri BM (1974) Morphogensis in mature endosperm cultures of Jatropha panduraefolia. Beitr Biol Pflanz 50:255–268

    Google Scholar 

  • Staubmann R, Ncube I, Gubitz GM et al (1999) Esterase and lipase activity in Jatropha curcas L. seeds. J Biotechnol 75:117–126

    Article  PubMed  CAS  Google Scholar 

  • Stripe F, Pession-Brizzi A, Lorenzoni E et al (1976) Studies on the proteins from the seeds of Croton tiglium and of Jatropha curcas. Biochem J 156:1–6

    Google Scholar 

  • Sujatha M (2006) Genetic improvement of Jatropha curcas (L.) possibilities and prospects. Indian J Agrofor 8:58–65

    Google Scholar 

  • Sujatha M, Dhingra M (1993) Rapid plant regeneration from various explants of Jatropha integerrima. Plant Cell Tiss Org Cult 35:293–296

    Article  Google Scholar 

  • Sujatha M, Mukta N (1996) Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas. Plant Cell Tiss Org Cult 44:135–141

    Article  Google Scholar 

  • Sujatha M, Prabakaran AJ (2003) New ornamental Jatropha hybrids through interspecific hybridization. Genet Resour Crop Evol 50:75–82

    Article  CAS  Google Scholar 

  • Sujatha M, Reddy TP (2000) Morphogenic responses of Jatropha integerrima explants to cytokinins. Biologia (Bratisl) 55:99–104

    CAS  Google Scholar 

  • Sujatha M, Makkar HPS, Becker K (2005) Shoot bud proliferation from axillary nodes and leaf sections of non-toxic Jatropha curcas L. Plant Growth Regul 47:83–90

    Article  CAS  Google Scholar 

  • Thepsamran N, Thepsithar C, Thongpukdee A (2008) In vitro induction of shoots and roots from Jatropha curcas L. explants. J Hortic Sci Biotechnol 83:106–112

    CAS  Google Scholar 

  • Thomas P (2004) In vitro decline in plant cultures: detection of a legion of covert bacteria as the cause for degeneration of long-term micropropagated triploid watermelon cultures. Plant Cell Tiss Org Cult 77:173–179

    Article  Google Scholar 

  • Van den Berg AJJ, Horsten SFAJ, van den Bosch Kettenes JJ et al (1995) Curcacycline A—a novel cyclic octapeptide isolated from the latex of Jatropha curcas L. FEBS Lett 358:215–218

    Article  PubMed  Google Scholar 

  • Varshney A, Johnson TS (2010) Efficient plant regeneration from immature embryo cultures of Jatropha curcas, a biodiesel plant. Plant Biotech Rep 4:139–148

    Article  Google Scholar 

  • Varshney A, Sangapillai R, Patil M et al (2011) Histological evidence of morphogenesis from various explants of Jatropha curcas L. Trees. doi:10.1007/s00468-011-0546-x

    Google Scholar 

  • Wang PJ, Charles A (1991) Micropropagation through meristem culture. In: Bajaj YPS (ed), High tech and micropropagation biotechnology in agriculture and forestry vol. 17, pp 32–52. Springer, Berlin Heidelberg

    Google Scholar 

  • Warakagoda PS, Subasinghe S (2009) In vitro culture establishment and shoot proliferation of Jatropha curcas L. Trop Agric Res Ext 12:77–80

    Google Scholar 

  • Wei T, Newton RJ (2004) Increase of polyphenol oxidase and decrease of polyamines correlate with tissue browning in Virginia pine (Pinus virginiana Mill). Plant Sci 167:621–628

    Article  CAS  Google Scholar 

  • Wei Q, Lu WD, Liao Y et al (2004) Plant regeneration from epicotyl explants of Jatropha curcas. J Plant Physiol Mol Biol 30:475–478

    Google Scholar 

  • Wei Q, Liao Y, Chen Y et al (2005) Isolation, characterisation and antifungal activity of β-1,3-glucanase from seeds of Jatropha curcas. South Afr J Bot 71:95–99

    CAS  Google Scholar 

  • Weida L, Qim W, Lin T et al (2003) Induction of callus from Jatropha curcas and its rapid propagation. Yingyong Yuhuan Jingshengwu Xuebao 9:127–130

    Google Scholar 

  • Weike C, Qi Z, Xingchun C et al (2006) Chemical modification of Jatropha curcas RIPs (curcin) and effect of the modification on relative activity of curcin. Chin J Appl Environ Biol 12:329–333

    Google Scholar 

  • Wu JH, Miller SA, Hall HK et al (2009) Factors affecting the efficiency of micropropagation from lateral buds and shoot tips of Rubus. Plant Cell Tiss Org Cult 99:17–25

    Article  CAS  Google Scholar 

  • Xu R, Wanga R, Liu A (2011) Expression profiles of genes involved in fatty acid and triacylglycerol synthesis in developing seeds of Jatropha (Jatropha curcas L.). Biomass Bioenerg. doi:10.1016/j.biombioe.2011.01.001

  • Yeung EC (1999) The use of histology in the study of plant tissue culture systems. Some practical comments. In Vitro Cell Dev Biol-Plant 35:137–143

    Article  Google Scholar 

  • Zhang Y, Wang Y, Jiang L (2007) Aquaporin JcPIP2 is involved in drought responses in Jatropha curcas. Acta Biochim Biophys Sin 39:787–794

    Article  PubMed  CAS  Google Scholar 

  • Zhang FL, Niu B, Wang YC et al (2008) A novel betaine aldehyde dehydrogenase gene from Jatropha curcas, encoding an enzyme implicated in adaptation to environmental stress. Plant Sci 174:510–518

    Article  CAS  Google Scholar 

  • Zhao Y, Zhou Y, Brian WWG (2005) Variation in leaf structures of micropropagated rhubarb (Rheum rhaponticum L.) PC49. Plant Cell Tiss Org Cult 85:115–121

    Article  Google Scholar 

  • Zong H, Wang S, Ouyang C et al (2010) Agrobacterium-mediated transformation of Jatropha curcas young leaf explants with lateral shoot-inducing factor (LIF). Intl J Agric Biol 12:891–896

    CAS  Google Scholar 

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Authors and Affiliations

  1. Directorate of Oilseeds Research, Rajendranagar, Hyderabad, Andhra Pradesh, 500 030, India

    M. Sujatha

  2. Department of Environmental Sciences, Bharathiar University, Coimbatore, Tamilnadu, 641 046, India

    S. Nithianantham

  3. Plant Stress Genomics Research Centre, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Kingdom of Saudi Arabia

    M. P. Reddy

Authors
  1. M. Sujatha
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  2. S. Nithianantham
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  3. M. P. Reddy
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Correspondence to M. Sujatha .

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Editors and Affiliations

  1. , Department of Agricultural, University of Helsinki, 5 Latokartanonkaari Room C 130, Helsinki, 00014, Finland

    Shri Mohan Jain

  2. Dept. Agricultural & Food Engineering, Indian Institute of Technology, Kharagpur, 721302, India

    S. Dutta Gupta

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Sujatha, M., Nithianantham, S., Reddy, M.P. (2013). Plant Regeneration and Genetic Transformation in Jatropha . In: Jain, S., Dutta Gupta, S. (eds) Biotechnology of Neglected and Underutilized Crops. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5500-0_13

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