Application of Plasmonics in Biophotonics: Laser and Nanostructures for Cell Manipulation
In cell biology and regenerative medicine, there is a certain need to modify or manipulate cells, for example within the field of tissue engineering or gene therapy.
Laser radiation allows the precise manipulation and imaging of cells with subcellular resolution. However, high numerical aperture objectives have to be used, to achieve spatial localization and confinement of the laser radiation. Thereby, cell throughput is limited. A possible technology to achieve a similar confinement of laser radiation can be the employment of plasmonic resonances. Using noble metals so-called surface plasmons, being collective electron oscillations at the surface of nanostructures or nanoparticles, can be excited by the laser radiation. Within the near field of these plasmons, the high field intensities can be used to achieve manipulation or characterization of biological processes within a cell.
KeywordsGold Nanoparticles Field Enhancement Lucifer Yellow Human Stem Cell Cell Manipulation
- 2.Arnold CL, Heisterkamp A, Ertmer W, Lubatschowski H (2008) Computational model for nonlinear plasma formation in high NA micromachining of transparent materials and biological cells. Opt Express 15(16):10304–10317Google Scholar
- 4.Koenig K, Riemann I, Fischer P, Halbhuber K (1999) Intracellular nanosurgery with near infrared femtosecond laser pulses. Cell Mol Biol 45:192–201Google Scholar
- 9.Mie G (1908) Optical properties of colloidal gold solutions. Ann Phys 25:329Google Scholar