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Ion Channels in Cardiac Fibroblasts: Link to Mechanically Gated Channels and their Regulation

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Mechanically Gated Channels and their Regulation

Part of the book series: Mechanosensitivity in Cells and Tissues ((MECT,volume 6))

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Abstract

Cardiac fibroblasts according to a number of authors have delayed rectifier current (IK), transient potassium current (Ito), inward rectifier potassium current (IKir), Ca2+-activated K+ current (IK(Ca)), TTX-sensitive sodium voltage-gated current (INa.TTX), TTX-sensitive sodium voltage-gated current (INa.TTXR), volume-sensitive chloride current (ICl.vol), voltage gated proton current (IHv), non-selective cation currents, besides mechanosensitive MG currents reported by Kamnkin et al. Manuscript describes single mechanically gated channels (MGCs), recorded simultaneously with whole cell MG currents. It demonstrates that cellular compression activates current, flowing through single MGCs (recorded in cell attached mode), along with whole cell MGC current (recorded in whole cell mode), which leads to generation of mechanically induced potentials (MIP). Cellular stretching inactivates those currents. Gd3+, cytochalasin D and colchicine inhibit both the whole-cell MG currents and single MG currents activity. All of those currents, which are mentioned above, together with MG currents contribute to alterations of fibroblast membrane potential (resting potentials and MIPs). Since fibroblasts are connected with cardiomyocytes via gap junctions, hyperpolarization of resting potential, triggered by cellular stretching, depolarization of the resting potentials, triggered by cellular compression, and the repolarization of the MIPs may potentially prolong the action potential duration in cardiomyocytes thereby predisposing the heart to arrhythmia.

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Acknowledgements

This work was supported by the Russian Foundation for Basic Research (grant no. 09–04-01277-a). Department of Fundamental and Applied Physiology (Professor and Chairman – Andre Kamkin) was supported by Ministry of Education and Science of the Russian Federation. The Order of Ministry of Education and Science of the Russian Federation No. 743 from 01 July 2010, Supplement, Event 4.4, the Period of Financing 2010–2019.

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  1. Department of Fundamental and Applied Physiology, Laboratory of Electrophysiology, Russian State Medical University, Ostrovitjanova 1, 117997, Moscow, Russia

    Denis V. Abramochkin, Ilya Lozinsky & Andre Kamkin

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  1. Denis V. Abramochkin
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  2. Ilya Lozinsky
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  3. Andre Kamkin
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Correspondence to Andre Kamkin .

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  1. Chemical Department, Moscow State University, Vorobiovy Gory MSU, Moscow, 119899, Russia

    Andre Kamkin

  2. , Fundamental and Applied Physiology, Russian State Medical University, Ostrovitjanova Str 1, Moscow, 117997, Russia

    Ilya Lozinsky

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Abramochkin, D.V., Lozinsky, I., Kamkin, A. (2012). Ion Channels in Cardiac Fibroblasts: Link to Mechanically Gated Channels and their Regulation. In: Kamkin, A., Lozinsky, I. (eds) Mechanically Gated Channels and their Regulation. Mechanosensitivity in Cells and Tissues, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5073-9_7

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