Ex Vivo Expansion of Stem and Progenitor Cells Using Thrombopoietin

  • Zoran Ivanovic
  • Marija Vlaski
Part of the Stem Cells and Cancer Stem Cells book series (STEM, volume 8)


Although most researchers predicted that thrombopoietin (TPO) would be a lineage specific regulator, with physiologic effects limited to megakaryocytes and platelets, it became clear that it is also an important growth factor of hematopoietic stem cells. This property of TPO qualified it for ex vivo expansion procedures aimed to amplify committed progenitors and stem cells, or, at least, to maintain the latter. During more than one decade of experimental work and preclinical development, the first efficient expansion procedures appeared enabling an acceleration of hematopoietic reconstitution after transplantation. All successful procedures were based on a cytokine cocktail containing TPO among other cytokines. The action of TPO on stem cells seems to be related to its hypoxia-mimicking properties.


Stem Cell Stem Cell Factor Platelet Production Cord Blood Cell Cytokine Cocktail 
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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  1. 1.Aquitaine-Limousin Branch of French Blood Institute, UMR 5164 CNRSUniversité SegalenBordeauxFrance

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