Retention of Stem Cell Properties Post-expansion in Myosphere Culture

  • Yan Wei
  • Andreas M. Kaufmann
  • Andreas E. Albers
Part of the Stem Cells and Cancer Stem Cells book series (STEM, volume 8)


Skeletal muscle has a prominent potential to regenerate after repeated trauma due to a pool of stem cells that reside in skeletal muscle. Satellite cells (SCs), as the primary source of this capability, originate in the embryonic dermamyotome and are located between the muscular basal membrane and the sarcolemma of mature adult skeletal muscle and compose the tissue-specific stem cell population. In response to muscle damage, SCs proliferation is triggered and they either differentiate and fuse to form new muscle fibers or alternatively self-renew to maintain the stem cell pool.

To facilitate the use of SCs in clinical scenarios, we need to explore the biology of these cells in greater detail. One clear goal is to be able to definitively identify and purify SCs. The myosphere forming assay is robust and reflects the behavior of SCs. Clonal analysis where single cells give rise to myospheres need to be used to follow the self-renewal and multipotency characteristics of SCs. Myosphere formation in combination with other markers of SCs behavior represents the state of the art to follow these cells. Remarkably, myospheres-derived progenitor cells (MDPCs) are not only able to differentiate into mesodermal cell types including the myogenic, adipogenic, osteogenic, cardiacogenic, and hematopoietic lineages, but also possess the potential to break germ layer commitment and differentiate into ectodermal lineages including neuron-like cells under certain conditions.


Stem Cell Satellite Cell Adult Skeletal Muscle Muscle Stem Cell Resident Stem Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Yan Wei
    • 1
  • Andreas M. Kaufmann
    • 2
  • Andreas E. Albers
    • 3
  1. 1.Department of Otolaryngology, Head and Neck SurgeryThe First Affiliated Hospital of Medicine School of Zhejiang UniversityHang ZhouChina
  2. 2.Clinic for GynecologyChaité-Universitätsmedizin BerlinBerlinGermany
  3. 3.Department of Otolaryngology, Head and Neck SurgeryChaité-Universitätsmedizin BerlinBerlinGermany

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