Phenotypic Evaluation of Spring Barley RIL Mapping Populations for Pre-harvest Sprouting, Fusarium Head Blight and β-Glucans
The overall objective of the research is to develop molecular markers which can be used in spring barley breeding. The aim of this study was to summarise phenotyping data from recombinant inbred line (RIL) populations for mapping the QTLs for resistance to pre-harvest sprouting and Fusarium head blight (FHB) as well as content of β-glucans. The field and laboratory experiments were performed at the State Priekuli Plant Breeding Institute and at the State Stende Cereal Breeding Institute for two seasons (2010–2011). The mapping populations for pre-harvest sprouting consist of 93 (RILs produced from a cross between hulless barley (HB) breeding line ‘PR 3642’ (susceptible) and HB variety ‘CDC Rattan’ (resistant), and of 94 RILs from a cross between HB variety ‘CDC Freedom’ (susceptible) and hulled variety ‘Samson’ (resistant). Eighty-six RILs for mapping resistance to FHB were derived from a cross between ‘Fontana’ (susceptible) and ‘ND 16461’ (resistant). The content of β-glucans was evaluated in 106 RIL population developed from the spring barley cross of HB lines ‘KM-1910’ (low content of β-glucans) and ‘KM-2084’ (high) and in 117 RILs developed from cross of hulled variety ‘Justina’ (low content of β-glucans) and ‘KM-2084’ (high). The noticeable variation among the recombinant inbred lines is found for all evaluated traits.
KeywordsSpring barley Pre-harvest sprouting FHB β-Glucans RIL Phenotyping
This study is performed with financial support of European Social Fund co-financed project 2009/0218/1DP/18.104.22.168.0/09/APIA/VIAA/099. Thanks to Dr. K. Vaculova, Dr. B. Rossnagel and Dr. H. Buerstmayr for providing genotypes used as parents in crosses.
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