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The Construction of Molecular Genetic Map of Barley Using SRAP Markers

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Advance in Barley Sciences

Abstract

Sequence-related amplified polymorphism (SRAP) markers, a novel PCR-based molecular marker technique, were successfully applied in map construction, cultivar identification, diversity evaluation, comparative genomics, and gene location of different plant species. The molecular genetic map of SRAP markers in Steptoe/Morex DH population was constructed in this study using 216 SRAP markers and 312 SSR markers. Twenty-one of 216 SRAP markers generated 78 polymorphic loci, and 98 of 312 SSR markers produced 107 polymorphic loci. Among the 185 loci, 175 loci (70 SRAP loci, 105 SSR loci) were assigned to nine linkage groups. The map covered 1,475 cM with a mean density of 8.7 cM per locus. Thirty-three of all the loci (17.84%) showed significant segregation distortion. Twenty-three of the 33 loci (69.7%) skewed toward the parent Steptoe, whereas the remaining loci (21.3%) deviated toward the parent Morex. And some of these distorted loci tended to cluster at the end of linkage groups, others dispersed on linkage groups in a decentralized fashion. The three putative segregation distortion regions (SDRs) were detected on chromosomes 2H, 4H, and 5H, respectively. This linkage map should indicate its importance in quantitative trait loci (QTLs) mapping, marker-assisted selection (MAS), and integrative analysis for further genetic studies with other linkage maps in barley.

Presenting author, Leilei L. Guo

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References

  • Becker, J., Vos, P., Kuiper, M., Salamini, F., & Heum, M. (1995). Combined mapping of AFLP and RFLP markers in barley. Molecular & General Genetics, 249, 65–73.

    Article  CAS  Google Scholar 

  • Budak, H., Shearman, R. C., Parmaksiz, I., & Dweikat, I. (2004). Comparative analysis of seeded and vegetative biotype buffalo grasses based on phylogenetic relationship using ISSRs, SSRs, RAPDs, SRAPs. Theoretical and Applied Genetics, 109, 280–288.

    Article  PubMed  CAS  Google Scholar 

  • Budak, H., Shearman, R. C., Gulsen, O., & Dweikat, I. (2005). Understanding ploidy complex and geographic origin of the buchloe dactyloides genome using cytoplasmic and nuclear marker system. Theoretical and Applied Genetics, 111, 1545–1552.

    Article  PubMed  CAS  Google Scholar 

  • Del Sal, G., Manfiloeti, G., & Schneider, C. (1989). The CTAB-DNA precipitation method: a common mini-scale preparation of template DNA from phagemids, phages or plasmids suitable for sequencing. BioTechniques, 7, 514–520.

    PubMed  Google Scholar 

  • Dizkirici, A., Zeki, K., Elif, Güren H., & Hikmet, Budak. (2010). Barley germplasms developed for scald disease resistance exhibited a high level of genetic diversity based on SRAP markers. Turkish Journal of Biology, 34, 271–279.

    CAS  Google Scholar 

  • Feng, Z. Y., Liu, X. J., Zhang, Y. Z., & Ling, H. Q. (2006). Genetic diversity analysis of Tibetan wild barley using SSR markers. Acta Genetica Sinica, 33, 917–928.

    Article  PubMed  CAS  Google Scholar 

  • Ferriol, M., Picó, B., & Nuez, F. (2003). Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theoretical and Applied Genetics, 107, 271–282.

    Article  PubMed  CAS  Google Scholar 

  • Graner, A., Jahoor, A., Schondelmaier, J., Siedler, H., Pillen, K., Fischbeck, G., Wenzel, G., & Herrmann, R. G. (1991). Construction of an RFLP map of barley. Theoretical and Applied Genetics, 83, 250–256.

    Article  Google Scholar 

  • Hearnden, P. R., Eckermann, P. J., McMichael, G. L., Hayden, M. J., Eglinton, J. K., & Chalmers, K. J. (2007). A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics, 115, 383–391.

    Article  PubMed  CAS  Google Scholar 

  • Heun, M., Kennedy, A. E., Anderson, J. A., Lapitan, N. L. V., Sorrells, M. E., & Tanksley, S. D. (1991). Construction of a restriction fragment length polymorphism map of barley (Hordeum vulgare). Genome, 34, 437–477.

    Article  Google Scholar 

  • Karakousis, A., Gustafson, J. P., Chalmers, K. J., Barr, A. R., & Langridge, P. (2003). A consensus map of barley integrating SSR, RFLP, and AFLP markers. Australian Journal of Agricultural Research, 54, 1173–1185.

    Article  CAS  Google Scholar 

  • Kosambi, D. D. (1944). The estimation of map distances from recombination values. Annals of Eugenics, 12, 172–175.

    Google Scholar 

  • Lander, E. S., Green, P., Abrahamson, J., Barlow, A., Daly, M. J., Lincoln, S. E., & Newberg, L. A. (1987). MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1, 174–181.

    Article  PubMed  CAS  Google Scholar 

  • Langridge, P., & Barr, A. R. (2003). Preface. Australian Journal of Agricultural Research, 54, i–iv.

    Article  Google Scholar 

  • Li, G., & Quiros, C. F. (2001). Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics, 103, 455–461.

    Article  CAS  Google Scholar 

  • Li, G., Gao, M., Yang, B., & Quiros, C. F. (2003). Gene for gene alignment between the Brassica and Arabidopsis genomes by direct transcriptome mapping. Theoretical and Applied Genetics, 107, 168–180.

    Article  PubMed  CAS  Google Scholar 

  • Li, L., Zheng, X. Y., & Lin, L. W. (2006). Analysis of genetic diversity and identification of cucumber varieties by SRAP. Molecular Plant Breeding, 4, 702–708.

    CAS  Google Scholar 

  • Lin, Z. X., Zhang, Y. X., Zhang, X. L., & Guo, X. P. (2009). A high-density integrative linkage map for Gossypium hirsutum. Euphytica, 166, 35–45.

    Article  Google Scholar 

  • Liu, L. W., Zhao, L. P., Gong, Y. Q., Wang, M. X., Chen, L. M., Yang, J. L., Wang, Y., Yu, F. M., & Wang, L. Z. (2008). DNA fingerprinting and genetic diversity analysis of late-bolting radish cultivars with RAPD, ISSR and SRAP markers. Scientia Horticulturae, 116, 240–247.

    Article  CAS  Google Scholar 

  • Liu, X. J., You, J. M., Guo, L. L., Liu, X. C., He, Y. J., Yuan, J. E., Liu, G. X., & Feng, Z. Y. (2011). Genetic analysis of segregation distortion of SSR markers in F2 population of barley. Journal of Agricultural Science, 3, 172–177.

    Google Scholar 

  • Sun, S. J., Gao, W., Lin, S. Q., Zhu, J., Xie, B. G., & Lin, Z. B. (2006). Analysis of genetic diversity in Ganoderma population with a novel molecular marker SRAP. Applied Microbiology and Biotechnology, 72, 537–543.

    Article  PubMed  CAS  Google Scholar 

  • Varshney, R. K., Marcel, T. C., Ramsay, L. R., Russell, J., Röder, M. S., Stein, N., Waugh, R., Langridge, P., Niks, R. E., & Graner, A. (2007). A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics, 114, 1091–1103.

    Article  PubMed  CAS  Google Scholar 

  • Wang, J. S., Yao, J. C., & Li, W. (2008). Construction of a molecular map for melon (Cucumis melo L.) based on SRAP. Frontiers of Agriculture in China, 2, 451–455.

    Article  Google Scholar 

  • Wenzl, P., Li, H. B., Carling, J., Zhou, M. X., Raman, H., Paul, E., Hearnden, P., Maier, C., Xia, L., Caig, V., Ovesná, J., Cakir, M., Poulsen, D., Wang, J. P., Raman, R., Smith, K. P., Muehlbauer, G. J., Chalmers, K. J., Kleinhofs, A., Huttner, E., & Kilian, A. (2006). A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits. BMC Genomics, 7, 206.

    Article  PubMed  Google Scholar 

  • Yang, P., Liu, X. J., Liu, X. C., Wang, X. W., He, S. P., Li, G., Yang, W. Y., & Feng, Z. Y. (2008). Genetic diversity analysis of the developed qingke (hulless barley) varieties from the plateau regions of Sichuan province in China revealed by SRAP markers. Hereditas (Beijing), 30, 115–122.

    Article  CAS  Google Scholar 

  • Yang, P., Liu, X. J., Liu, X. C., Yang, W. Y., & Feng, Z. Y. (2010). Diversity analysis of the developed qingke (hulless barley) cultivars representing different growing regions of the Qinghai-Tibet Plateau in China using sequence-related amplified polymorphism (SRAP) markers. African Journal of Biotechnology, 9, 8530–8538.

    CAS  Google Scholar 

  • Zhang, F., Chen, S. M., Chen, F. D., Fang, W. M., Chen, Y., & Li, F. T. (2011). SRAP-based mapping and QTL detection for inflorescence-related traits in chrysanthemum (Dendranthema morifolium). Molecular Breeding, 27, 11–23.

    Article  CAS  Google Scholar 

  • Zheng, J., Zhang, Z. S., Chen, L., Wan, Q., Hu, M. C., Wang, W., Zhang, K., Liu, D. J., Chen, X., & Wei, X. Q. (2008). Intron-targeted intron-exon splice conjunction (IT-ISJ) marker and its application in construction of upland cotton linkage map. Agricultural Sciences in China, 7, 1172–1180.

    Article  Google Scholar 

  • Zohary, D., & Hopf, M. (1988). Domestication of plants in the old world (3rd ed.). Oxford: Clarendon.

    Google Scholar 

Download references

Acknowledgments

We thank J. M., Yang X. Y., and Yuan J. E. for some technical experiments and Dr. Yang P. (IPK, Germany) for his assistance in data analysis. The present work was funded by grants from China Agriculture Research System (CARS-05) and also Major Project from Sichuan Education Department in China.

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Authors and Affiliations

  1. Barley Research Centre, Department of Plant Genetics and Breeding, College of Agronomy, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, 611130, Chengdu, Sichuan, China

    Leilei L. Guo, Xianjun J. Liu, Xinchun C. Liu, Zhimin M. Yang, Deyuan Y. Kong, Yujing J. He & Zongyun Y. Feng

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  1. Leilei L. Guo
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  2. Xianjun J. Liu
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  3. Xinchun C. Liu
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  4. Zhimin M. Yang
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  7. Zongyun Y. Feng
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Correspondence to Zongyun Y. Feng .

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Editors and Affiliations

  1. Agronomy Department, Zhejiang University, Hangzhou, 310029, China, People's Republic

    Guoping Zhang

  2. Dept. Agriculture Western Australia, Government of Western Australia, Baron Hay-Court 3, South Perth, 6151, West Australia, Australia

    Chengdao Li

  3. , Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 South Street, Zhongguancun, Beijing, 100081, China, People's Republic

    Xu Liu

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© 2013 Zhejiang University Press and Springer Science+Business Media Dordrecht

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Guo, L.L. et al. (2013). The Construction of Molecular Genetic Map of Barley Using SRAP Markers. In: Zhang, G., Li, C., Liu, X. (eds) Advance in Barley Sciences. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4682-4_37

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