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Side-View Endomicroscopy for High-Resolution In Vivo Imaging of the Gastrointestinal Tract

  • Pilhan Kim
  • Euiheon Chung
  • Rakesh K. Jain
  • Seok H. Yun
  • Dai Fukumura
Chapter

Abstract

Small animal, particularly mouse, models are a useful tool for basic and translational studies of human diseases. However, it has been challenging to optically dissect gastrointestinal tract disease models including colorectal cancer and inflammatory bowel disease. To this end, we have developed a novel side-view endomicroscope that allows for non-invasive, real-time, cellular-level visualization in gastrointestinal tracts of live, anesthetized mice. Here we describe a procedure to visualize both the microvasculature and fluorescently labeled cells in the gastrointestinal mucosa. By obtaining wide-area images at multiple time points in a genetically engineered mouse model of spontaneous colon carcinoma, we can monitor the process of angiogenesis associated with tumor development in real time at the orthotopic site. This powerful new imaging method can provide novel insights into many aspects of gastrointestinal diseases.

Keywords

Optical Coherence Tomography Intravital Microscopy Engineer Mouse Model Grin Lens Orthotopic Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This article is based on the previous article: Kim P, Chung E, Yamashita H et al (2010) In vivo wide-area cellular imaging by side-view endomicroscopy. Nat Methods 7:303–305.The work described here was supported by grants from Wellman Center for Photomedicine, Human Frontier Science Program, Tosteson Fellowship, National Research Foundation of Korea, National Institutes of Health, National Science Foundation, and United States Army. E. Chung is supported by the Institute of Medical System Engineering of the GIST, and the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) (No. 2011–0019619, 2011–0019632).

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Pilhan Kim
    • 1
    • 3
  • Euiheon Chung
    • 2
    • 4
  • Rakesh K. Jain
    • 2
  • Seok H. Yun
    • 1
    • 3
  • Dai Fukumura
    • 2
  1. 1.Wellman Center for Photomedicine, Department of DermatologyHarvard Medical School and Massachusetts General HospitalBostonUSA
  2. 2.Edwin L. Steele Laboratory for Tumor Biology, Department of Radiation OncologyHarvard Medical School and Massachusetts General HospitalBostonUSA
  3. 3.Graduate School of Nanoscience and TechnologyKorea Advanced Institute of Science and TechnologyDaejeonRepublic of Korea
  4. 4.Department of Medical System Engineering and School of MechatronicsGwangju Institute of Science and TechnologyGwangjuRepublic of Korea

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