Ischemia created in the animals were long been used as a model to study ischemia mediated effects in vivo. Pathological angiogenesis is the key hallmark of various ischemic diseases where blood vessel formation was compromised due to low blood flow. New blood vessels form in order to compensate the low blood perfusion in the ischemic area. This neovascularization and remodeling of the existent vessels protect from the consequences of ischemia associated diseases like myocardial infarction and stroke. A better understanding of the mechanisms of functional vessel formation is a pre-requisite to improve the treatment of ischemic pathologies. Therefore, the research area warrants an easily accessible model in which vessel formation can be both manipulated and studied. However, a limited number of efforts have been put forward yet to develop an ischemia models where ischemia mediated remodeling of vessels can be studied in real time. In present study, we used 4 day grown chick embryo to ligate right vitelline artery and create partial ischemia in the vascular bed of the embryo. The model has been developed based on the principle that blocking blood flow in the vascular bed will stop the nutrient and oxygen supply to the adjacent vessels and thus creating an ischemia like condition. Additionally, ischemia related changes in angiogenesis can be followed and tracked in real time in the vascular bed of the chick embryo. The present ex vivo model can be utilized in studying ischemia related angiogenesis in specific and hypoxia and/or low oxygen mediated angiogenesis in general.
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This work was supported by grants from KB Chandrasekhar-Research Foundation and from Indian Council Of Medical Research (No. 51/2/2009-Ana-BMS to SC).
Clark EB, Hu N (1982) Developmental hemodynamic changes in the chick embryo from stage 18 to 27. Circ Res 51:810–815PubMedCrossRefGoogle Scholar
Nakazawa M, Miyagawa S, Nishibatake M et al (1988) Hemodynamic characteristics in neural crest cell-excised chick embryo. Heart Vessels 4:136–140PubMedCrossRefGoogle Scholar
Hogers B, DeRuiter MC, Gittenberger-de Groot AC et al (1997) Unilateral vitelline vein ligation alters intracardiac blood flow patterns and morphogenesis in the chick embryo. Circ Res 80:473–481PubMedCrossRefGoogle Scholar
Hogers B, DeRuiter MC, Gittenberger-de Groot AC et al (1999) Extraembryonic venous obstructions lead to cardiovascular malformations and can be embryolethal. Cardiovasc Res 41:87–99PubMedCrossRefGoogle Scholar
Kolluru GK, Sinha S, Majumder S et al (2010) Shear stress promotes nitric oxide production in endothelial cells by sub-cellular delocalization of eNOS: a basis for shear stress mediated angiogenesis. Nitric Oxide 22:304–315PubMedCrossRefGoogle Scholar
Stewart DE, Kirby ML, Sulik KK (1986) Hemodynamic changes in chick embryos precede heart defects after cardiac neural crest ablation. Circ Res 59:545–550PubMedCrossRefGoogle Scholar
Majumder S, Ilayaraja M, Seerapu HR et al (2010) Chick embryo partial ischemia model: a new approach to study ischemia ex vivo. PLoS One 5:e10524PubMedCrossRefGoogle Scholar
Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92CrossRefGoogle Scholar
Vos SS, Ursem NTC, Hop WM et al (2003) Acutely altered hemodynamics following venous obstruction in the early chick embryo. J Exp Biol 206:1051–1057CrossRefGoogle Scholar
Jiang M, Wang B, Wang C et al (2008) Angiogenesis by transplantation of HIF-1 alpha modified EPCs into ischemic limbs. J Cell Biochem 103:321–334PubMedCrossRefGoogle Scholar
Lee SH (2000) Early expression of angiogenesis factors in acute myocardial ischemia and infarction. N Engl J Med 342:626–633PubMedCrossRefGoogle Scholar
Bergeron M, Yu AY, Solway KE et al (1999) Induction of hypoxia-inducible factor-1 (HIF-1) and its target genes following focal ischaemia in rat brain. Eur J Neurosci 11:4159–4170PubMedCrossRefGoogle Scholar
Wang WZ, Fang XH, Stephenson LL et al (2008) Ischemia/reperfusion-induced necrosis and apoptosis in the cells isolated from rat skeletal muscle. J Orthop Res 26:351–356PubMedCrossRefGoogle Scholar
Niemistö A, Dunmire V, Yli-Harja O et al (2005) Robust quantification of in vitro angiogenesis through image analysis. IEEE Trans Med Imaging 24:549–553PubMedCrossRefGoogle Scholar
Tamilarasan KP, Kolluru GK, Rajaram M et al (2006) Thalidomide attenuates nitric oxide mediated angiogenesis by blocking migration of endothelial cells. BMC Cell Biol 7:17PubMedCrossRefGoogle Scholar