Abstract
The objective of this work was to assess the genomic constitution and intergeneric recombination in three successive unselected generations, (F2–F4), derived from an intergeneric hybrid between Festuca pratensis Huds. (2n = 4x = 28) and Lolium perenne L. (2n = 4x = 28). Examination based on genomic in situ hybridization analyses of randomly chosen plants in each generation indicated progressive changes in genome balance towards that of Lolium. The dominance of Lolium chromatin over Festuca likely resulted from extensive recombination between chromosomes of the parental genomes, together with substitutions of whole Festuca chromosomes by whole Lolium chromosomes. The total number of Lolium chromosomes increased from generation to generation. The number of recombinant chromosomes, and recombination breakpoints per genotype, also increased in successive generations, but their number was higher for Festuca than for Lolium. The patterns of genome constitutions and recombination were similar to those we observed in selected generations (F2–F4 breeding populations) developed from the same F1 hybrid plants.
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Acknowledgements
This work was supported by the Polish Ministry of Science and Higher Education (Grant no. N N310 090736).
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Zwierzykowski, Z., Książczyk, T., Taciak, M., Zwierzykowska, E., Jones, N., Kosmala, A. (2013). Genome Constitution in Selected and Unselected Plants of F2–F4 Generations Derived from an Allotetraploid Festuca pratensis × Lolium perenne Hybrid. In: Barth, S., Milbourne, D. (eds) Breeding strategies for sustainable forage and turf grass improvement. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4555-1_9
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DOI: https://doi.org/10.1007/978-94-007-4555-1_9
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