Green Tea and Its Major Polyphenol EGCG Increase the Activity of Oral Peroxidases

  • Baruch Narotzki
  • Yishai Levy
  • Dror Aizenbud
  • Abraham Z. ReznickEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 756)


Oral peroxidases (OPO) consist mainly of salivary peroxidase and myeloperoxidase and are involved in oral defense mechanisms. Salivary peroxidase is synthesized and secreted by salivary glands, whereas myeloperoxidase is found in polymorphonuclear leukocytes, which migrate into the oral cavity at gingival crevices. Green tea is the world’s second most popular drink after water. Polyphenols are the most biologically active group of tea components. The purpose of our study was to elucidate the interaction between green tea & EGCG (Epigallocatechin 3-gallate), its main polyphenol and OPO. In previous studies we have shown that elderly trained people who drink green tea for 3 months, have a higher level of OPO activity compared to non-drinkers. Thus, we decided to extend our project in order to understand the above observations by studying the interaction of green tea and OPO both in vitro and in vivo. Addition of green tea and black tea infusions (50 μl/ml) and EGCG (50 μM) to saliva, resulted in a sharp rise of OPO activity +280% (p = 0.009), 54% (p = 0.04) and 42% (p = 0.009), respectively. The elevation of OPO activity due to addition of green tea and EGCG was in a dose dependent manner: r = 0.91 (p = 0.001) and r = 0.637 (p = 0.019), respectively. Also, following green tea infusion mouth rinsing, a rise of OPO activity was observed: +268% (p = 0.159). These results may be of great clinical importance, as tea consumer’s oral epithelium may have better protection against the deleterious effects of hydroxyl radicals, produced by not removed hydrogen peroxides in the presence of metal ions. Higher OPO activity upon green tea drinking may provide an extra protection against oxidative stress in the oral cavity.


Oral peroxidases Salivary peroxidase Myeloperoxidase Green tea Epigallocatechin 3-gallate Polymorphonuclear leukocytes Saliva 



Supported by the Krol foundation of Barnegat NJ, USA, Rappaport Institute for Research and Myers-JDC-Brookdale Institute of Gerontology and Human Development and Eshel-the Association for the Planning and Development of Services for the Aged in Israel.

Conflicts of interest: The authors declare no conflicts of interest in relation to this article.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Baruch Narotzki
    • 1
  • Yishai Levy
    • 2
    • 3
  • Dror Aizenbud
    • 2
    • 4
  • Abraham Z. Reznick
    • 1
    Email author
  1. 1.Department of Anatomy and Cell BiologyThe Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of TechnologyHaifaIsrael
  2. 2.The Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of TechnologyHaifaIsrael
  3. 3.Department of Medicine DRambam Health Care CampusHaifaIsrael
  4. 4.Orthodontic and Craniofacial Center, Graduate School of DentistryRambam Health Care CampusHaifaIsrael

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