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Systems Metabolic Engineering pp 271–327Cite as

Bridging Omics Technologies with Synthetic Biology in Yeast Industrial Biotechnology

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Abstract

Metabolic engineering, defined as the practice of manipulating cells’ genetic and regulatory processes for improving cellular performance, has been recently integrating systems and synthetic biology with other technologies, e.g. molecular biology, physiology, biochemistry, analysis science, bioinformatics and biochemical engineering. The aim is to surmount cells’ performance-related limitations, e.g. metabolic limitation of cellular processes, network rigidity and global regulation, in a comprehensive, rational and high-throughput manner. Using this multi-level/integrated approach, the (re)design and (re)construction of microbial systems for the development of novel products with significant impact on current global problems, e.g. depletion of energy resources and global warming, is becoming a reality.

In the last two decades, technological platforms for systems biology, e.g. genomics, transcriptomics, proteomics, metabolomics and fluxomics, and synthetic biology approaches, e.g. synthetic biological parts, devices and systems, have been implemented and efficiently used as tools for metabolic engineering of high-value bioproducts. One successful story on how metabolic engineering has been used to enhance the synthesis of microbial-based molecules is the production of biofuels. In this review, synthetic and systems biology technologies for yeast metabolic engineering will be described in detail. In addition, two case studies related with biofuel production (ethanol and 1-butanol) in yeast S. cerevisiae will be presented.

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Acknowledgements

The authors acknowledge the financial support received from the EU Framework VII project SYSINBIO (www.sysbio.se/sysinbio), the European Research Council, Knut and Alice Wallenberg Foundation and the Chalmers foundation.

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  1. Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 412 96, Gothenburg, Sweden

    António Roldão, Il-Kwon Kim & Jens Nielsen

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  3. Jens Nielsen
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Correspondence to Jens Nielsen .

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  1. Inst. Bioverfahrenstechnik, TU Braunschweig, Gaußstraße 17, Braunschweig, 38106, Germany

    Christoph Wittmann

  2. Science and Technology-KAIST, Dept. Biosystems a. Bioinformatics, Korea Advanced Institute, Guseong-dong 373-1, Daejeon, 305-701, Korea, Republic of (South Korea)

    Sang Yup Lee

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Roldão, A., Kim, IK., Nielsen, J. (2012). Bridging Omics Technologies with Synthetic Biology in Yeast Industrial Biotechnology. In: Wittmann, C., Lee, S. (eds) Systems Metabolic Engineering. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4534-6_9

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