Bridging Omics Technologies with Synthetic Biology in Yeast Industrial Biotechnology

Chapter

Abstract

Metabolic engineering, defined as the practice of manipulating cells’ genetic and regulatory processes for improving cellular performance, has been recently integrating systems and synthetic biology with other technologies, e.g. molecular biology, physiology, biochemistry, analysis science, bioinformatics and biochemical engineering. The aim is to surmount cells’ performance-related limitations, e.g. metabolic limitation of cellular processes, network rigidity and global regulation, in a comprehensive, rational and high-throughput manner. Using this multi-level/integrated approach, the (re)design and (re)construction of microbial systems for the development of novel products with significant impact on current global problems, e.g. depletion of energy resources and global warming, is becoming a reality.

In the last two decades, technological platforms for systems biology, e.g. genomics, transcriptomics, proteomics, metabolomics and fluxomics, and synthetic biology approaches, e.g. synthetic biological parts, devices and systems, have been implemented and efficiently used as tools for metabolic engineering of high-value bioproducts. One successful story on how metabolic engineering has been used to enhance the synthesis of microbial-based molecules is the production of biofuels. In this review, synthetic and systems biology technologies for yeast metabolic engineering will be described in detail. In addition, two case studies related with biofuel production (ethanol and 1-butanol) in yeast S. cerevisiae will be presented.

Keywords

Systems biology Genomics Metabolomics Fluxomics Proteomics Transcriptomics Illumina sequencing Microarrays RNAseq Data mining Genome-scale metabolic models Synthetic biology Targeted gene expression Multi-gene assembly Synthetic pathway Promoter libraries Riboregulators Riboswitches Ribozymes Protein scaffolds Promoter shuffling Promoter binding proteins Antisense RNA Metabolic engineering Yeast Saccharomyces cerevisiae Industrial biotechnology High-value bioproducts Renewable resources Biofuels Ethanol Butanol 

Notes

Acknowledgements

The authors acknowledge the financial support received from the EU Framework VII project SYSINBIO (www.sysbio.se/sysinbio), the European Research Council, Knut and Alice Wallenberg Foundation and the Chalmers foundation.

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© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  1. 1.Department of Chemical and Biological EngineeringChalmers University of TechnologyGothenburgSweden

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