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Gene Site-Specific Insertion in Plants

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Site-directed insertion of transgenes

Part of the book series: Topics in Current Genetics ((TCG,volume 23))

Abstract

Gene site-specific insertion is an important tool to precisely integrate DNA molecules at a defined genomic location. Gene Targeting has many important implications in plant biotechnology such as creation of novel plant traits or the study of gene function. It requires the insertion of an extrachromosomal DNA into the plant cell that will be further integrated at a defined genomic locus through the use of the plant endogenous recombination machinery. Extensive efforts have been made to understand the mechanisms governing gene targeting and for establishing efficient system to achieve precise and efficient targeting. In particular the recent development of engineered nucleases represents an important step forward towards allowing the insertion of a transgene at any given locus in the genome.

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Acknowledgements

We would like to thank the members of the recombination group for fruitful discussions and critical reading of the manuscript and apologise to all colleagues whose valuable work was only cited through review. Work in our laboratory is supported by a European Union research grant (FP7 KBBE-2008-227190, RECBREED), the Centre National de la Recherche Scientifique, the Université Blaise Pascal, the Université d’ Auvergne and the Institut National de la Santé et de la Recherche Médicale.

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  1. Génétique, Reproduction et Développement, UMR CNRS 6293 – Clermont Université – INSERM U1103, Centre National de Recherche Scientifique/Université Blaise Pascal, 24 avenue des Landais, 80026, 63171, Aubière CEDEX, France

    Olivier Da Ines & Charles I. White

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  1. Olivier Da Ines
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Da Ines, O., White, C.I. (2013). Gene Site-Specific Insertion in Plants. In: Renault, S., Duchateau, P. (eds) Site-directed insertion of transgenes. Topics in Current Genetics, vol 23. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4531-5_11

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