Advertisement

Cysteine Synthesis in the Chloroplast Is Not Required for Resistance of Arabidopsis thaliana to H2S Fumigation

  • Hannah Birke
  • Markus Wirtz
  • Luit J. De Kok
  • Rüdiger Hell
Conference paper
Part of the Proceedings of the International Plant Sulfur Workshop book series (PIPSW, volume 1)

Abstract

Reduced sulfur is necessary for synthesis of various essential compounds in the plant cell including cysteine and glutathione. For this reason sulfide is continuously produced in plastids as intermediate of the assimilatory sulfate reduction pathway. Varying demand for sulfide during development and in response to external stress challenges a plant to rapidly re-organize the entire sulfur metabolism, since high endogenous sulfide levels are potentially toxic for the cellular metabolism. Upon prolonged exposure of Arabidopsis to 1 μl l–1 H2S, the endogenous sulfide level in the shoot was kept low, whereas there was a mass increase in thiol content. The cysteine and glutathione content increased approximately 30- and 3.4-fold, respectively. Notably, the dramatic changes on thiol levels were not accompanied by changes in extractable activities of the cysteine synthesizing enzymes, serine acetyltransferase and O-acetylserine(thiol)lyase (OAS-TL). It was evident that, despite the production of sulfide in plastids, its consumption by OAS-TL in the same compartment was not crucial for adaptation of Arabidopsis to high H2S levels in the environment.

Keywords

Sulfide Content Cysteine Content Sulfide Level Cysteine Synthesis High Sulfide Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

H.B. is affiliated with The Hartmut Hoffmann-Berling International Graduate School of Molecular and Cellular Biology, University of Heidelberg, Germany, and the graduate program ENDS; the authors gratefully acknowledge support by DFG grant He 1848/14-1 and the Schmeil Stiftung Heidelberg.

References

  1. De Kok LJ, Durenkamp M, Yang L, Stulen I (2007) Atmospheric sulfur. In: Hawkesford MJ, De Kok LJ (eds) Sulfur in plants – an ecological perspective. Springer, Dordrecht, The Netherlands pp 91–106Google Scholar
  2. Haas FH, Heeg C, Queiroz R, Bauer A, Wirtz MA, Hell R (2008) Mitochondrial serine acetyltransferase functions as a pacemaker of cysteine synthesis in plant cells. Plant Physiol 148:1055–1067PubMedCrossRefGoogle Scholar
  3. Heeg C, Kruse C, Jost R, Gutensohn M, Ruppert T, Wirtz M, Hell R (2008) Analysis of the Arabidopsis O-acetylserine(thiol)lyase gene family demonstrates compartment-specific differences in the regulation of cysteine synthesis. Plant Cell 20:168–185PubMedCrossRefGoogle Scholar
  4. Koralewska A, Stuiver CEE, Posthumus FS, Kopriva S, Hawkesford MJ, De Kok LJ (2008) Regulation of sulfate uptake, expression of the sulfate transporters Sultr1;1 and Sultr1;2, and APS reductase in Chinese cabbage (Brassica pekinensis) as affected by atmospheric H2S nutrition and sulfate deprivation. Funct Plant Biol 35:318–327CrossRefGoogle Scholar
  5. Nakamura M, Kasugai I, Abe M, Youssefian S (2009) Increased thiol biosynthesis of transgenic poplar expressing a wheat O-acetylserine(thiol) lyase enhances resistance to hydrogen sulfide and sulfur dioxide toxicity. Plant Cell Rep 28:313–323PubMedCrossRefGoogle Scholar
  6. Völkel S, Grieshaber MK (1994) Oxygen dependent sulfide detoxification in the lugworm Arenicola marina. Mar Biol 118:137–147CrossRefGoogle Scholar
  7. Watanabe M, Mochida K, Kato T, Tabata S, Yoshimoto N, Noji M, Saito K (2008) Comparative genomics and reverse genetics analysis reveal indispensable functions of the serine acetyltransferase gene family in Arabidopsis. Plant Cell 20:2484–2496PubMedCrossRefGoogle Scholar
  8. Wirtz M, Hell R (2006) Functional analysis of the cysteine synthase protein complex from plants: structural, biochemical and regulatory properties. J Plant Physiol 163:273–286PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Hannah Birke
    • 1
  • Markus Wirtz
    • 1
  • Luit J. De Kok
    • 2
  • Rüdiger Hell
    • 1
  1. 1.Department for Plant Molecular Biology, Centre for Organismal Studies (COS)University of HeidelbergHeidelbergGermany
  2. 2.Laboratory of Plant PhysiologyUniversity of GroningenGroningenNetherlands

Personalised recommendations