Simultaneous Determination of Sulfur Metabolites in Plant Tissue by LC-ESI-MS/MS

  • Mei-Hwei Tseng
  • Luit J. De Kok
  • Yao-Moan Huang
Conference paper
Part of the Proceedings of the International Plant Sulfur Workshop book series (PIPSW, volume 1)


An analytical method was developed for the accurate quantification of the contents of sulfur metabolites of plant tissues based on liquid chromatography – electrospray ionization – tandem mass spectrometry (LC-ESI-MS/MS). 34S-Labeled sulfur metabolites were biosynthesized in Arabidopsis thaliana from 34S-SO 4 2− and used as internal standards for the analysis and quantification of these sulfur-containing metabolites in A. thaliana and O. banksiaefolia fern spores.


Flower Stem Fern Spore GSSG Content Photon Fluence Rate Sulfur Metabolite 
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The authors acknowledge Dr. Ding-Tzai Li for fruitful discussions and Mass Solution Technology Co., Ltd for excellent technical support.


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Mei-Hwei Tseng
    • 1
  • Luit J. De Kok
    • 2
  • Yao-Moan Huang
    • 3
  1. 1.Department of Applied Physics and ChemistryTaipei Municipal University of EducationTaipeiTaiwan
  2. 2.Laboratory of Plant PhysiologyUniversity of GroningenGroningenThe Netherlands
  3. 3.Division of SilvicultureTaiwan Forestry Research InstituteTaipeiTaiwan

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