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Preliminary Characteristics of a Tobacco Gene Down-Regulated by Sulfur Deprivation and Encoding a Cys-Rich Protein

  • Małgorzata Lewandowska
  • Katarzyna Zientara-Rytter
  • Agnieszka Sirko
Conference paper
Part of the Proceedings of the International Plant Sulfur Workshop book series (PIPSW, volume 1)

Abstract

Tobacco cDNA clones representing genes differentially regulated by the short-term sulfur deficit were identified with the suppression subtractive hybridization (SSH) method. Many of them encoded proteins with established function, however for some of them not only their role in sulfur deficit response remained elusive but assigning them to any cellular processes was impossible. The down-regulated D6 clone was representing one of such genes. The first steps of analysis included cloning of the full-length mRNA sequence and the sequence of its promoter region. Subsequent work, including monitoring of D6 expression in various growth conditions, its intracellular localization and database screening for homologues lead to the hypothesis that D6 may take part in differentiation and developing processes. However, its precise role and rationale for down-regulation during sulfur deficit remain unclear.

Keywords

Suppression Subtractive Hybridization Genetic Tumor Stimulate Wound Healing Suppression Subtractive Hybridization Method Unnamed Protein Product 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The work was supported by the Ministry of Science and Higher Education (N N302 119435). ML acknowledges support by German Academic Exchange Service (DAAD).

References

  1. Bristow J, Carey W, Egging D, Schalkwijk J (2005) Tenascin-X, collagen, elastin, and the Ehlers–Danlos syndrome. Am J Med Genet C Semin Med Genet 139C:24–30PubMedCrossRefGoogle Scholar
  2. Demidova-Rice TN, Geevarghese A, Herman IM (2011) Bioactive peptides derived from vascular endothelial cell extracellular matrices promote microvascular morphogenesis and wound healing in vitro. Wound Repair Regen 19:59–70PubMedCrossRefGoogle Scholar
  3. Egging D, van Vlijmen-Willems I, van Tongeren T, van Tongeren T, Schalkwijk J, Peeters A (2007) Wound healing in tenascin-X deficient mice suggests that tenascin-X is involved in matrix maturation rather than matrix deposition. Connect Tissue Res 48:93–98PubMedCrossRefGoogle Scholar
  4. Fujita T, Kouchi H, Ichikawa T, Syono K (1994) Cloning of cDNAs for genes that are specifically or preferentially expressed during the development of tobacco genetic tumors. Plant J 5:645–654PubMedCrossRefGoogle Scholar
  5. Lewandowska M, Sirko A (2009) Identification of additional genes regulated by sulfur shortage in tobacco. In: Sirko A, De Kok LJ, Haneklaus S, Hawkesford MJ, Rennenberg H, Saito K, Schnug E, Stulen I (eds) Sulfur metabolism in plants. Regulatory aspects, significance of sulfur in the food chain, agriculture and the environment. Backhuys Publishers, Leiden, Margraf Publishers GmbH, Weikersheim, pp 73–77Google Scholar
  6. Lewandowska M, Wawrzyńska A, Kamińska J, Liszewska F, Sirko A (2005) Identification of novel proteins of Nicotiana tabacum regulated by short term sulfur starvation. In: Saito K, De Kok LJ, Stulen I, Hawkesford MJ, Schnug E, Sirko A, Rennenberg H (eds) Sulfur transport and assimilation in plants in the postgenomic era. Backhuys Publishers, Leiden, pp 153–156Google Scholar
  7. Lewandowska M, Wawrzyńska A, Moniuszko G, Łukomska J, Zientara K, Piecho M, Hodurek P, Zhukov I, Liszewska F, Nikiforova V, Sirko A (2010) A contribution to identification of novel regulators of plant response to sulfur deficiency: characteristics of a tobacco gene UP9C, its protein product and the effects of UP9C silencing. Mol Plant 3:347–360PubMedCrossRefGoogle Scholar
  8. Maruyama-Nakashita A, Nakamura Y, Tohge T, Saito K, Takahashi H (2006) Arabidopsis SLIM1 is a central transcriptional regulator of plant sulfur response and metabolism. Plant Cell 18:3235–3251PubMedCrossRefGoogle Scholar
  9. Meiniel O, Meiniel R, Lalloue F, Didier R, Jauberteau M-O, Meiniel A, Petit D (2008) The lengthening of a giant protein: when, how, and why? J Mol Evol 66:1–10PubMedCrossRefGoogle Scholar
  10. Nacken WK, Huijser P, Beltran JP, Saedler H, Sommer H (1991) Molecular characterization of two stamen-specific genes, tap1 and fil1, that are expressed in the wild type, but not in the deficiens mutant of Antirrhinum majus. Mol Gen Genet 229:129–136PubMedCrossRefGoogle Scholar
  11. Wawrzyńska A, Lewandowska M, Hawkesford MJ, Sirko A (2005) Using a suppression subtractive library-based approach to identify tobacco genes regulated in response to short-term sulphur deficit. J Exp Bot 56:1575–1590PubMedCrossRefGoogle Scholar
  12. Wawrzyńska A, Lewandowska M, Sirko A (2010) Nicotiana tabacum EIL2 directly regulates expression of at least one tobacco gene induced by sulphur starvation. J Exp Bot 61:889–900PubMedCrossRefGoogle Scholar
  13. Zientara-Rytter K, Łukomska J, Moniuszko G, Gwozdecki R, Surowiecki P, Lewandowska M, Liszewska F, Wawrzyńska A, Sirko A (2011) Identification and functional analysis of Joka2, a tobacco member of the family of selective autophagy cargo receptors. Autophagy 7:1145–1158PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Małgorzata Lewandowska
    • 1
    • 2
  • Katarzyna Zientara-Rytter
    • 1
  • Agnieszka Sirko
    • 1
  1. 1.Institute for Biochemistry and BiophysicsPolish Academy of SciencesWarsawPoland
  2. 2.Heidelberg Institute for Plant SciencesUniversity of HeidelbergHeidelbergGermany

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