Preliminary Characteristics of a Tobacco Gene Down-Regulated by Sulfur Deprivation and Encoding a Cys-Rich Protein
Tobacco cDNA clones representing genes differentially regulated by the short-term sulfur deficit were identified with the suppression subtractive hybridization (SSH) method. Many of them encoded proteins with established function, however for some of them not only their role in sulfur deficit response remained elusive but assigning them to any cellular processes was impossible. The down-regulated D6 clone was representing one of such genes. The first steps of analysis included cloning of the full-length mRNA sequence and the sequence of its promoter region. Subsequent work, including monitoring of D6 expression in various growth conditions, its intracellular localization and database screening for homologues lead to the hypothesis that D6 may take part in differentiation and developing processes. However, its precise role and rationale for down-regulation during sulfur deficit remain unclear.
KeywordsSuppression Subtractive Hybridization Genetic Tumor Stimulate Wound Healing Suppression Subtractive Hybridization Method Unnamed Protein Product
The work was supported by the Ministry of Science and Higher Education (N N302 119435). ML acknowledges support by German Academic Exchange Service (DAAD).
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