Glycogen Metabolism Regulation in Neurospora crassa
Microorganisms accumulate glycogen as carbon and energy reserves to face adverse environmental conditions during growth and development. The processes of glycogen synthesis and degradation share similarities among different microorganisms. However, the regulation of the metabolism as a whole shows differences, likely due to the environmental conditions to which they individually respond. This chapter aims to present some molecular mechanisms that regulate glycogen metabolism in the fungus Neurospora crassa. The availability of its genome sequence (Galagan et al., Nature 422:859–868, 2003) and a collection of mutant strains, each carrying a deletion in a specific ORF, allowed investigation into the role of specific proteins as regulators of glycogen metabolism to begin. Here we present some biochemical and molecular mechanisms that have already been described for this fungus, and additionally, we focused on more recent findings including the molecular basis underlying the metabolism regulation, mainly at transcriptional level.
KeywordsHeat Shock Neurospora Crassa Glycogen Phosphorylase Glycogen Metabolism Glycogen Accumulation
The work in the author’s laboratory was supported by grants and fellowships from FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), and CAPES (Coorde nação de Aperfeiçoamento de Pessoal de Nível Superior).
- Gonçalves RD, Cupertino FB, Freitas FZ, Luchessi AD, Bertolini MC (2011) A genome-wide screen for Neurospora crassa transcription factors regulating glycogen metabolism. Mol Cell Proteomics 10(11). doi: 10.1074/mcp.M111.007963
- Johnston M, Carlson M (1992) Regulation of carbon and phosphate utilization. In: Jones EW, Pringle JR, Broach JR (eds) The molecular and cellular biology of the yeast Saccharomyces. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 193–281Google Scholar
- Nowak AJ, Alfieri C, Stirnimann CU, Rybin V, Baudin F, Ly-Hartig N, Lindner D, Müller CW (2011) Chromatin-modifying complex component Nurf55/p55 associates with histones H3 and H4 and polycomb repressive complex 2 subunit Su(z)12 through partially overlapping binding sites. J Biol Chem 286:23388–23396PubMedCrossRefGoogle Scholar
- Roach PJ, Skurat AV, Harris RA (2001) Regulation of glycogen metabolism. In: Jefferson LS, Cherrington AD (eds) Handbook of physiology. The endocrine pancreas and regulation of metabolism, vol II. Oxford University Press, New York, pp 609–647Google Scholar