Adherens Junctions and Pathogen Entry
Epithelia are highly organised structures protecting underlying tissues against microbial pathogens. Epithelial morphogenesis and maintenance is mediated by cell-cell adhesion molecules organised in junctional complexes, such as the adherens junctions. The tight organisation of these complexes and their interactions with cellular factors render the epithelia impermeable to potential invaders. Nevertheless, pathogens have developed strategies to target, interact and manipulate junctional complexes, in order to disrupt or cross the epithelial barriers and cause infection. Bacteria, viruses and parasites access the junctional molecular components either directly, often taking advantage of physiological alterations in epithelial polarity, or indirectly, by delivering into cells molecular factors that destabilise junctional integrity. Importantly, microbial interactions with junctional components are instrumental not only to elucidate mechanisms of invasion, but also to unravel fundamental physiological properties of the epithelial barriers, at the cellular and tissular level.
KeywordsTight Junction Adherens Junction Junctional Complex Shigella Flexneri Epithelial Sheet
Georgios Nikitas was a recipient of the Marie Curie Actions Early Stage Researcher INTRAPATH programme which he received while preparing his PhD thesis in the Laboratory of Microbes and Host Barriers, under the direction of Marc Lecuit in the Pasteur Institute.
- Attali C, Durmort C, Vernet T, Di Guilmi AM (2008) The interaction of Streptococcus prneumoniae with plasmin mediates transmigration across endothelial and epithelial monolayers by intercellular junction cleavage Infect Immun 76:5350–5356Google Scholar
- Oliveira MJ, Costa AM, Costa AC, Ferreira RM, Sampaio P, Machado JC, Seruca R, Mareel M, Figueiredo C (2009) CagA associates with c-Met, E-cadherin, and p120-catenin in a multiproteic complex that suppresses Helicobacter pylori-induced cell-invasive phenotype. J Infect Dis 200:745–755PubMedCrossRefGoogle Scholar
- Pentecost M, Kumaran J, Ghosh P, Amieva MR (2010) Listeria monocytogenes internalin B activates junctional endocytosis to accelerate intestinal invasion. PLoS Pathog 6(5): e1000900. doi:10.1371/journal.ppat.1000900Google Scholar
- Pentecost M, Otto G, Theriot JA, Amieva MR (2006) Listeria monocytogenes invades the epithelial junctions at sites of cell extrusion. PLoS Pathog 2(1): e3. doi:10.1371/journal.ppat.0020003Google Scholar
- Warner MS, Geraghty RJ, Martinez WM, Montgomery RI, Whitbeck JC, Xu R, Eisenberg RJ, Cohen GH, Spear PG (1998) A cell surface protein with herpesvirus entry activity (HveB) confers susceptibility to infection by mutants of herpes simplex virus type 1, herpes simplex virus type 2, and pseudorabies virus. Virology 246:179–189.PubMedCrossRefGoogle Scholar