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Technologies for Medical Sciences pp 243–266Cite as

Supercritical Solvent Impregnation of Natural Bioactive Compounds in N-Carboxybutylchitosan and Agarose Membranes for the Development of Topical Wound Healing Applications

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Part of the book series: Lecture Notes in Computational Vision and Biomechanics ((LNCVB,volume 1))

Abstract

Supercritical Solvent Impregnation (SSI) was used to load topical membrane-type wound dressing biomaterials with natural based bioactive compounds namelly quercetin as an antiinflammatory and thymol as anaesthetic and skin permeation enhancer. The biodegradable and biocompatible membranes where prepared as film- and foam-like structures of N-carboxybutylchitosan and agarose to study the influence of morphological structure on the fluid handling capacities of the materials. Results show that SSI is a feasible and advantageous process that permits to ‘tune’ the relative loaded amounts of the bioactive substances by changing the operational conditions. The process also promotes the size reduction of quercetin particles with a significant improvement in its solubility in aqueous solutions and consequently in its bioavailability. The prepared materials present a sustained delivery for quercetin and adequate fluid handling capacities that are in the typical and desired ranges for commercial wound dressings.

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Authors and Affiliations

  1. CIEPQPF, Chemical Engineering Department, FCTUC, University of Coimbra, Rua Sílvio Lima, Pólo II – Pinhal de Marrocos, 3030-790, Coimbra, Portugal

    A. M. A. Dias, M. E. M. Braga, I. J. Seabra & H. C. de Sousa

  2. ESAC, Politechnic Institute of Coimbra, Bencanta, 3040-316, Coimbra, Portugal

    I. J. Seabra

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  1. A. M. A. Dias
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  2. M. E. M. Braga
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  3. I. J. Seabra
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  4. H. C. de Sousa
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Correspondence to A. M. A. Dias .

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  1. Porto, 4200-465, Portugal

    Renato M. Natal Jorge

  2. Fac. Engenharia, Lab. Engenharia de Processos,, Universidade do Porto, Rua Dr. Roberto Frias, Porto, 4200-465, Portugal

    João Manuel R. S. Tavares

  3. UFMG, Laboratorio de Bioengenharia, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, Belo Horizonte, 31270-901, Minas Gerais, Brazil

    Marcos Pinotti Barbosa

  4. University of Dundee, College of Art, Science and Engineering, Medical Engineering Research Institute, Dundee, DD1 4 HN, United Kingdom

    A.P. Slade

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Dias, A.M.A., Braga, M.E.M., Seabra, I.J., de Sousa, H.C. (2012). Supercritical Solvent Impregnation of Natural Bioactive Compounds in N-Carboxybutylchitosan and Agarose Membranes for the Development of Topical Wound Healing Applications. In: Natal Jorge, R., Tavares, J., Pinotti Barbosa, M., Slade, A. (eds) Technologies for Medical Sciences. Lecture Notes in Computational Vision and Biomechanics, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4068-6_12

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