Separation of Amino Acids, Peptides, and Proteins by Ion Exchange Chromatography

  • Tanja Cirkovic Velickovic
  • Jana Ognjenovic
  • Luka Mihajlovic


Separation of amino acids, peptides, and proteins (bioanalytes) via ion exchange (IE) has widespread usage because it is usually very simple to design and it has high capacity and easily achievable control of the separation process. Amino acids, as principal constituents of proteins and having a plethora of biological functions of their own, are always in focus when developing novel methods. Separation and quantification of amino acids is essential in food science, medicine, agricultural science, etc. Peptides exist in nature and have diverse functions. Digestion of proteins by enzymes also gives complex mixtures of peptides and IE finds its application in peptide separation. There are lots of reasons for the popularity of IE in protein isolation and purification. It is used in research, analysis, and large-scale purification of proteins. Ion exchange is ideal for the initial capture of proteins because of its high capacity, relatively low cost, and its ability to survive rigorous cleaning regimes. This chapter covers basic principles and modern applications of IE in separation of amino acids, peptides, and proteins.


Monolithic Column Sepharose Fast Flow Peptide Separation Halophilic Protein Mugwort Pollen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors acknowledge the support of the Ministry of Science and Technological Development of the Republic of Serbia (Grant No. 172024).


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© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Tanja Cirkovic Velickovic
    • 1
  • Jana Ognjenovic
    • 1
  • Luka Mihajlovic
    • 1
  1. 1.University of BelgradeBelgradeSerbia

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