Abstract
Visualize the invisible is the most recent challenge of modern biotechnology in which the advancement of molecular and cell imaging has been prioritized. Fluorescence microscopy has become an essential tool to study biological molecules, pathways and events in vitro and in vivo for diagnostics and therapeutics. The field not only involves the development of new generation of molecules that are nontoxic and noninvasive, but also concerns their long-term stability, sensitivity and resolution in visual assays. This chapter reviews the use of quantum dots (small, light-emitting semiconductor nanocrystals) in conjugation with the anti-mortalin cell-internalizing antibody for bioimaging. The conjugate was able to enter the cells and illuminate them. The use of such illuminated cells for long-term tracking and imaging in mouse and rabbit models of cartilage and bone differentiation in vitro and in vivo is described.
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Acknowledgments
The authors thank Z. Kaul, Y. Ohyabu, T. Yoshioka, S. Sakai, H. Mishima, N. Ochiai and K. Inoue for their collaboration, technical assistance and encouragement through this work.
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Uemura, T., Nishi, M., Kaul, S.C., Wadhwa, R. (2012). Cell Internalizing Anti-Mortalin Antibody for Generation of Illuminating MSCs for Long-Term In vitro and In vivo Tracking. In: Kaul, S., Wadhwa, R. (eds) Mortalin Biology: Life, Stress and Death. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-3027-4_19
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DOI: https://doi.org/10.1007/978-94-007-3027-4_19
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