Chondrogenesis from Human Mesenchymal Stem Cells: Role of Culture Conditions
Tissue engineering has experienced significant advances in recent years. The application of cell-based tissue engineering approaches to articular cartilage repair has been a primary focus because articular cartilage is avascular and without a significant source of reparative cells. With recent advances in MSC biology, cell-based repair or regeneration of articular cartilage is becoming a practical possibility. However, isolation, and chondrogenic differentiation of the MSCs require further optimization.
Three phases are critical for MSC-based cartilage tissue engineering: isolation of the primary cells, mitotic expansion, and chondrogenic differentiation. Multiple variables play significant roles in each of these phases. Media composition, growth factor supplementation, and scaffold effects are just a few examples of the culture-condition variables critical to the success of MSC-based cartilage tissue engineering. These variables and their effects on MSC isolation, expansion, and differentiation will be discussed in this chapter.
KeywordsTissue Engineering Articular Cartilage Chondrogenic Differentiation Tissue Culture Plastic Scaffold Material
The authors wish to acknowledge funding from the National Institutes of Health AR050208 and AR053622. We thank Ms. Kitsie Penick, Ms. Lori Duesler, and Ms. Chih-Ling Chou for technical assistance. We also wish to acknowledge the contributions to the field of MSC chondrogenesis by the hundreds of researchers who could not be cited due to formatting constraints on this chapter.
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