Scaffolds for Human Dental Stem Cells to Regenerate Cementum

  • Jong Hoon Chung
  • Pill-Hoon Choung
  • Ki-Taek Lim
  • Han-Wool Choung
Part of the Stem Cells and Cancer Stem Cells book series (STEM, volume 5)


The basic strategy of tooth bioengineering involves the utilization of artificial extracellular matrix as scaffolds, in combination with specific cells under the stimulation of growth factors. Dental stem cells can successfully regenerated dental hard tissue according to the properties of scaffolds. The authors made synthetic hydroxyapatite (HA) from human tooth and named it as “toothapatite (TA)” because it was almost composed of HA and whitelockite. Through our research, biocompatibility, biodegradability and osteoconductivity of TA have been proven acceptable in vitro and in vivo. Therefore, TA can be anticipated as one of the adequate scaffold sources for culturing dental stem cells/dental cells. As degradable ‘bioceramics’, TA and β-tricalciumphosphate (TCP) were used with dental stem cells, in particular periodontal ligament stem cells (PDLSCs) and dental follicle stem cells (DFSCs) to regenerate cementum. The PDLSCs showed cellular cementum and DFSCs showed cementum–like mineralized tissues.

Polymer can serve as a framework for maintaining the shape of the defect so as to facilitate the regeneration of tissue. Poly-DL-lactide (PDLLA), as a degradable ‘polymer’, was added to bioceramics, creating TA/TCP/PDLLA composite scaffolds. The novel degradable bioceramic-polymer scaffolds, which have taken the merits of both bioceramics and polymer were fabricated, and the effects of these scaffolds seeded with human dental stem cells were investigated in vitro and in vivo. Like TA/TCP scaffolds, the bioceramic-polymer groups showed regenerated cementum-like minera­lized tissue in vivo. Thus, TA/TCP/PDLLA scaffolds can be used as novel potential scaffolds to regenerate cementum in tooth bioengineering.


Calcium Phosphate Composite Scaffold Amorphous Calcium Phosphate Dental Hard Tissue Dental Follicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Barrere F, Layrolle P, van Blitterswijk CA, de Groot K (1999) Biomimetic calcium phosphate coatings on Ti6AI4V: a crystal growth study of octacalcium phosphate and inhibition by Mg2+ and HCO3-. Bone 25:107S–111SPubMedCrossRefGoogle Scholar
  2. Ben-Nissan B (2003) Natural bioceramics: from coral to bone and beyond. Curr Opin Solid State Mater Sci 7:283–288CrossRefGoogle Scholar
  3. Bos RR, Rezema FR, Boering G, Nijenhuis AJ, Pennings AJ, Verwey AB, Nieuwenhuis P, Jansen HW (1991) Degradation of and tissue reaction to biodegradable poly(L-lactide) for use as internal fixation of fractures. Biomaterials 12:32–36PubMedCrossRefGoogle Scholar
  4. Buma P, Schreurs W, Verdonschot N (2004) Skeletal tissue engineering-from in vitro studies to large animal models. Biomaterials 25:1487–1495PubMedCrossRefGoogle Scholar
  5. Cui L, Liu B, Liu G, Zhang W, Cen L, Sun J, Yin S, Liu W, Cao Y (2007) Repair of cranial bone defects with adipose derived stem cells and coral scaffold in a canine model. Biomaterials 28:5477–5486PubMedCrossRefGoogle Scholar
  6. Duailibi SE, Duailibi MT, Vacanti JP, Yelick PC (2006) Prospects for tooth regeneration. Periodontol 2000 43:177–187CrossRefGoogle Scholar
  7. Erisken C, Kalyon DM, Wang H (2008) Functionally graded electrospun polycaprolactone and beta-tricalcium phosphate nanocomposites for tissue engineering appli­cations. Biomaterials 29:4065–4073PubMedCrossRefGoogle Scholar
  8. Freed LE, Marquis JC, Nohria A, Emmanual J, Mikos AG, Langer R (1993) Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers. J Biomed Mater Res 27:11–23PubMedCrossRefGoogle Scholar
  9. Gough JE, Notingher I, Hench LL (2004) Osteoblast attachment and mineralized nodule formation on rough and smooth 45S5 bioactive glass monoliths. J Biomed Mater Res A 68:640–650PubMedCrossRefGoogle Scholar
  10. Harada H, Kettunen P, Jung HS, Mustonen T, Wang YA, Thesleff I (1999) Localization of putative stem cells in dental epithelium and their association with Notch and FGF signaling. J Cell Biol 147:105–120PubMedCrossRefGoogle Scholar
  11. Hench LL (1996) Ceramics, glasses and glass-ceramics. In: Biomaterials science: an introduction to materials in medicine, 1st edn. Academic, San Diego, pp 81–83Google Scholar
  12. Hoh KY (1984) A study on the physical properties and cytotoxicity of tooth ash and dental porcelain. J Korean Acad Prosthodont 22:51–63Google Scholar
  13. Ji YM, Jeon SH, Park JY, Chung JH, Choung YH, Choung PH (2010) Dental stem cell therapy with calcium hydro­xide in dental pulp capping. Tissue Eng Part A 16:1823–1833PubMedCrossRefGoogle Scholar
  14. Jo YY, Lee HJ, Kook SY, Choung HW, Park JY, Chung JH, Chung YH, Kim ES, Yang HC, Choung PH (2007) Iso­lation and characterization of postnatal stem cells from human dental tissues. Tissue Eng Part A 13:767–775Google Scholar
  15. Kang YH, Jeon SH, Park JY, Chung JH, Choung YH, Choung HW, Kim ES, Choung PH (2011) Platelet-rich fibrin is a bioscaffold and reservoir of growth factors for tissue regeneration. Tissue Eng Part A 17:349–359PubMedCrossRefGoogle Scholar
  16. Karageorgiou V, Kaplan D (2005) Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 26(27):5474–5491PubMedCrossRefGoogle Scholar
  17. Kesenci K, Fambri L, Migliaresi C, Piskin E (2000) Pre­pa­ration and properties of poly(L-lactide)/hydroxyapatite composites. J Biomater Sci Polym Ed 11:617–632PubMedCrossRefGoogle Scholar
  18. Li X, Xie J, Yuan X, Xia Y (2004) Coating electrospun poly(epsilon-caprolactone) fibers with gelatin and calcium phosphate and their use as biomimetic scaffolds for bone tissue engineering. Langmuir 24:14145–14250CrossRefGoogle Scholar
  19. Lim KT, Chung HW, Im AL, Kim JH, Cho CS, Choung YH, Jeon SH, Choung PH, Chung JH (2010) Novel composite scaffolds for tooth regeneration using human dental pulp stem cells. J Tissue Eng Regen Med 6:1410–1419Google Scholar
  20. Lim KT, Suh JD, Kim JH, Choung PH, Chung JH (2011) Calcium phosphate bioceramics fabricated from extracted human teeth for tooth tissue engineering. J Biomed Mater Res B Appl Biomater 99(2):399–411PubMedGoogle Scholar
  21. Mikos AG, Bao Y, Cima LG, Ingber DE, Vacanti JP, Langer R (1993) Preparation of poly(glycolic acid) bonded fiber structures for cell attachment and transplantation. J Biomed Mater Res 27:183–189PubMedCrossRefGoogle Scholar
  22. Nakashima M, Akamine A (2005) The application of tissue engineering to regeneration of pulp and dentin in endodontics. Pulp Dentin Regen 31:711–718Google Scholar
  23. Nakashima M, Reddi AH (2003) The application of bone morphogenetic proteins to dental tissue engineering. Nat Biotechnol 21:1025–1032PubMedCrossRefGoogle Scholar
  24. Park JY, Jeon SH, Choung PH (2011) Efficacy of periodontal stem cell transplantation in the treatment of advanced periodontitis. Cell Transplant 20:271–285PubMedCrossRefGoogle Scholar
  25. Santavirta S, Konttinen YT, Saito T, Gronblad M, Partio E, Kempponen P, Rokkanen P (1990) Immune response to polyglycolic acid implants. J Bone Joint Surg 72:567–600Google Scholar
  26. Shi S, Bartold PM, Miura M, Seo BM, Robey PG, Gronthos S (2005) The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Orthod Craniofac Res 8:191–199PubMedCrossRefGoogle Scholar
  27. Sun H, Wu C, Dai K, Chang J, Tang T (2006) Proliferation and osteoblastic differentiation of human bone marrow-derived stromal cells on akermanite-bioactive ceramics. Biomaterials 27:5651–5657PubMedCrossRefGoogle Scholar
  28. Ural E, Kesenci K, Fambri L, Migliaresi C, Piskin E (2000) Poly(D, L-cactide/ε-caprolactone)/hydroxyapatite composites. Biomaterials 21:2147–2154PubMedCrossRefGoogle Scholar
  29. Verrier S, Blaker JJ, Maquet V, Hench LL, Boccaccini AR (2004) PDLLA/Bioglass composites for soft-tissue and hard-tissue engineering: an in vitro cell biology assessment. Biomaterials 25:3013–3021PubMedCrossRefGoogle Scholar
  30. Yamada Y (2004) Autogenous injectable bone for regeneration with mesenchymal stem cells and platelet-rich plasma: tissue engineered bone regeneration. Tissue Eng Part A 10:955–964Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Jong Hoon Chung
    • 1
  • Pill-Hoon Choung
    • 1
  • Ki-Taek Lim
    • 1
  • Han-Wool Choung
    • 1
  1. 1.Department of Oral and Maxillofacial Surgery, Dental research Institute, School of DentistrySeol National UniversitySeolSouth Korea

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