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Cardiac Stem Cells Derived from Epithelial-Mesenchymal Transition of the Epicardial Cells: Role in Heart Regeneration (Method)

  • Daria Nurzynska
  • Franca Di Meglio
  • Stefania Montagnani
  • Clotilde Castaldo
Chapter
Part of the Stem Cells and Cancer Stem Cells book series (STEM, volume 5)

Abstract

Epithelial-mesenchymal transition (EMT) involves genotypic and phenotypic changes leading to the conversion of polarized epithelial cells into motile mesenchymal cells. This process occurs typically in mesothelial cells of developing cardiac tissue, but recent discoveries indicate the preservation or reactivation of embryonic potential of epicardial cells in the adult heart. Moreover, cells with cardiac stem cell phenotype and properties have been identified among epicardially derived cell (EPDC) population, indicating EMT and EPDCs as yet another source of cells for adult cardiac tissue regeneration in cardiovascular diseases. Understanding how and when EPDCs arise from epicardial cells by EMT in the adult human heart could have a strong impact in clarifying the mechanisms of cardiac tissue self-renewal and regeneration. The chapter includes a protocol for adult human epicardial cell culture and EMT induction leading to a formation of cardiac progenitor cells in vitro.

Keywords

Hepatocyte Growth Factor Mesothelial Cell Cardiac Stem Cell Cardiac Progenitor Cell Epicardial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Daria Nurzynska
    • 1
  • Franca Di Meglio
    • 1
  • Stefania Montagnani
    • 1
  • Clotilde Castaldo
    • 1
  1. 1.Department of Biomorphological and Functional SciencesUniversity of Naples “Federico II”NaplesItaly

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