Bio-Decontamination of Water and Surfaces by DC Discharges in Atmospheric Air
Two types of DC-driven atmospheric air discharges, including a streamer corona and a transient spark with short high current pulses of limited energy, were employed for bio-decontamination of water and various surfaces (agar plates, plastic foils, human teeth) contaminated by bacteria or spores (Salmonella typhimurium, Bacillus cereus). Both discharges generate cold non-equilibrium plasma. The discharges combined with the electro-spraying of the treated water through the needle electrode lead to fast and efficient bio-decontamination. Experiments comparing direct and indirect plasma effects, oxidation stress measurements in the cell membranes, and chemical changes induced in the treated water enable assessment of the plasma agents being responsible for microbial inactivation. Radicals and reactive oxygen species seem to be dominant biocidal agents, although deeper understanding of the plasma-induced water chemistry and of the temporal evolution of the bio-inactivation processes is needed.
KeywordsTooth Surface Human Tooth Negative Corona Streamer Corona Indirect Exposure
Effort sponsored by Slovak grant agency VEGA 1/0668/11 and 1/0711/09, Slovak Research and Development Agency APVV SK-CZ-0179-09. The human teeth were provided by patients of Dr. O. Šipoldová. We thank P. Lukeš (IPP Prague) for his motivating ideas, and I. Jedlovský and B. Pongrác (FMFI Bratislava) for assistance.
- 13.Dobrynin D, Fridman G, Mukhin YV, Wynosky-Dolfi MA, Rieger J, Rest RF, Gutsol AF, Fridman A (2010) Cold plasma inactivation of Bacillus cereus and Bacillus anthracis (Anthrax) Spores. IEEE Trans Plasma Sci 39:1878–1883Google Scholar
- 28.Yu QS, Huang C, Hsieh F, Huff HE, Duan YX (2007) Bacterial inactivation using a low-temperature atmospheric plasma brush sustained with argon gas. J Biomed Mater Res B Appl Biomater 80:211–219Google Scholar