Abstract
A low temperature non-equilibrium air plasma spray is tested as a blood coagulator. Emission spectroscopy of the plasma effluent indicates that it carries abundant reactive atomic oxygen (RAO), which can activate erythrocyte – platelet interactions to enhance blood coagulation for plug formation. Tests of the device for wound bleeding control were performed on pigs. Four types of wounds, straight cut and cross cut in the ham area, a hole in an ear saphenous vein, and a cut to an ear artery, were examined. The results showed that this plasma spray could effectively stop the bleeding and reduced the bleeding time considerably. Post-Operative observation of straight cut and cross cut wound healing was carried out. It was found that the plasma treatment had a positive impact on wound healing, in particular, of the cross cut wound; its healing time was shortened by a half.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Jevon P, Cooper L (2008) First aid. Part 5. First-aid treatment for severe bleeding. Nurs Times 104:26–7
Perkins JG, Cap AP, Weiss BM, Reid TJ, Bolan CD (2008) Massive transfusion and nonsurgical hemostatic agents. Crit Care Med 36(Suppl 7):S325–39
Vargo JJ (2004) Clinical applications of the argon plasma coagulator. Gastrointest Endosc 59:81–88
Raiser J, Zenker M (2006) Argon plasma coagulation for open surgical and endoscopic applications: state of the art. J Phys D Appl Phys 39:3520–3523
Malick KJ (2006) Clinical applications of argon plasma coagulation in endoscopy. Gastroenterol Nurs 29:386–391
Ambrosio G, Oriente A, Napoli C, Palumbo G, Chiariello P, Marone G, Condorelli M, Chiariello M, Triggiani M (1994) Oxygen radicals inhibit human plasma acetylhydrolase, the enzyme that catabolizes platelet-activating factor. J Clin Invest 93:2408–16
Golino P, Ragni M, Cirillo P, Avvedimento VE, Feliciello A, Esposito N, Scognamiglio A, Trimarco B, Iaccarino G, Condorelli M, Chiariello M, Ambrosio G (1996) Effects of tissue factor induced by oxygen free radicals on coronary flow during reperfusion. Nature Med 2:35–40
Kalghatgi SU, Fridman G, Cooper M, Nagaraj G, Peddinghaus M, Balasubramanian M, Vasilets VN, Gutsol AF, Fridman A, Friedman G (2007) Mechanism of blood coagulation by nonthermal atmospheric pressure dielectric barrier discharge plasma. IEEE Trans Plasma Sci 35:1559–1566
Fridman G, Peddinghaus M, Ayan H, Fridman A, Balasubramanian M, Gutsol A, Brooks AD, Friedman G (2006) Blood coagulation and living tissue sterilization by floating electrode dielectric barrier discharge in air. Plasma Chem Plasma Process 26:425–442
Chen CY, Fan HW, Kuo SP, Chang JH, Pedersen T, Mills T, Huang CC (2009) Blood clotting by low temperature air plasma. IEEE Trans Plasma Sci 37:993–999
Kuo SP, Tarasenko O, Chang JH, Popovic S, Chen CY, Fan HW, Scott A, Lahiani M, Alusta P, Drake JD, Nikolic M (2009) Contribution of a portable air plasma torch to rapid blood coagulation as a method of preventing bleeding. New J Phys 11:115016, 17 pp
Kuo SP (2010) Portable plasma sterilizer. US Patent US7777151
Kuo SP, Pedersen T, Mills T (2008) Lateral distribution of atomic oxygen flux produced by an array of three fan-shaped plasma torches. IEEE Trans Plasma Sci 36:1056–1057
Kuo SP, Chen CY, Lin CS, Chiang SH (2010) Wound bleeding control by low temperature air plasma. IEEE Trans Plasma Sci 38:1908–1914
Oda T, Yamashita Y, Takezawa K, Ono R (2006) Oxygen atom behavior in the nonthermal plasma. Thin Solid Films 506–507:669–673
Georg A, Engemann J, Brockhaus A (2002) Investigation of a pulsed oxygen microwave plasma by time-resolved two-photon allowed laser-induced fluorescence. J Phys D Appl Phys 35:875–881
Henderson WR, Fite WL, Brackmann RT (1969) Dissociative attachment of electrons to hot oxygen. Phys Rev 183:157–166
Finazzi-Agro’ A, Menichelli A, Persiani M, Biancini G, Del Principe D (1982) Hydrogen peroxide release from human blood platelets. Biochim Biophys Acta 718:21–25
Del Principe D, Menichelli A, De Matteis W, Di Giulio S, Giordani M, Savini I, Finazzi-Agro A (1991) Hydrogen peroxide is an intermediate in the platelet activation cascade triggered by collagen, but not by thrombin. Thromb Res 62:365–75
Del Principe D, Menichelli A, De Matteis W, Di Corpo ML, Di Giulio S, Finazzi-Agro A (1985) Hydrogen peroxide has a role in the aggregation of human platelets. FEBS Lett 185:142–6
Praticò D, Iuliano L, Alessandri C, Camastra C, Violi F (1993) Polymorphonuclear leukocyte-derived O2-reactive species activate primed platelets in human whole blood. Am J Physiol 264:H1582–H1587
Davis JC, Hunt TK (1988) Problem wounds: the role of oxygen. Elsevier, New York
La Van FB, Hunt TK (1990) Oxygen and wound healing. Clin Plast Surg 17(3):463–72
Acknowledgements
We are grateful to Alessandro Betti for fabricating the plasma spray device. This work was supported in part by a NYU-Poly seed Grant and in part by Adventix Technologies Inc.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media B.V.
About this paper
Cite this paper
Kuo, S.P., Chen, CY., Lin, CS., Chiang, SH. (2012). Non-equilibrium Air Plasma for Wound Bleeding Control. In: Machala, Z., Hensel, K., Akishev, Y. (eds) Plasma for Bio-Decontamination, Medicine and Food Security. NATO Science for Peace and Security Series A: Chemistry and Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2852-3_27
Download citation
DOI: https://doi.org/10.1007/978-94-007-2852-3_27
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-007-2851-6
Online ISBN: 978-94-007-2852-3
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)